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Home / Equine Research Bank / Vet Res Commun / Horses as a source of bioactive fecal strains Enterococcus m...
Veterinary research communications2022; 46(3); 739-747; doi: 10.1007/s11259-022-09893-9

Horses as a source of bioactive fecal strains Enterococcus mundtii.

Abstract: Bacteriocin-producing bacteria with probiotic character are known as nutritional supplements mainly for livestock. Among those beneficial bacteria we also found enterococci. Because the species strains Enterococcus mundtii also can produce bacteriocins, this study was focused on fecal strains E. mundtii from horses and their bioactivity with a view to their possible future use in breeding. Rectal removal from 47 horses (40 mares and 7 stallions), the Norik breed from Muráň were sampled in eastern Slovakia during November 2019 year. Horses age ranged from five months up to 23 years. Using MALDI-TOF mass spectrometry and 16S rRNA sequences analysis, 14 strains were allotted to the species E. mundtii. Bacteriocin substances produced by the strains EMKD 38/1, EMKD 40/2, EMKD 34/2 and EMKD 41/3 showed inhibitory activity against the most susceptible (principal) indicator strain Enterococcus avium EA5 and against listeriae as well (inhibitory activity from 100 up to 1 600 AU/mL). Only strain EMKD 41/3 possess Ent P and Mundticin KS genes and showed the broadest inhibitory activity. Ent B gene possessing strain EMKD 24/1 inhibited a growth of only indicator strain EA5. Identified E. mundtii tolerate low pH 3 and oxgall/bile. They were hemolysis, gelatinase and DNase negative and mostly susceptible to clinical antibiotics which are properties requested for application potential of strain. Substance from the strain with the broadest antimicrobial spectrum showed its practical/application potential, e.g. for optimizing the host microbiota which is important regarding the maintenance of animal`s health status.
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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Publication Date: 2022-02-11 PubMed ID: 35147847PubMed Central: 2737909DOI: 10.1007/s11259-022-09893-9Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study that investigates the effectiveness of bacteriocin-producing bacteria, specifically Enterococcus mundtii strains, found in horse feces, in inhibiting pathogenic bacteria, with the aim of potentially using these strains to enhance livestock breeding.

About the Study

  • This research focused on finding strains of the bacterium Enterococcus mundtii in horse feces that produce bacteriocins, substances that can kill other bacteria. This is because these strains have the potential for use in livestock breeding as a probiotic supplement.
  • To carry out the study, feces were collected rectally from 47 horses, made up of 40 mares and 7 stallions from the Norik breed. These horses, ranging in age from 5 months to 23 years, were located in eastern Slovakia and the sampling was done in November 2019.

Findings

  • Utilising MALDI-TOF mass spectrometry and 16s rRNA sequences analysis, the researchers were able to identify 14 strains of E. mundtii out of the samples collected.
  • The bacteriocins produced by four particular strains (EMKD 38/1, EMKD 40/2, EMKD 34/2 and EMKD 41/3) demonstrated inhibitory activity against Enterococcus avium EA5, known to be the most susceptible strain, and listeria, with their inhibitory activity ranging from 100 to 1,600 AU/mL.
  • The EMKD 41/3 strain, which contain the Ent P and Mundticin KS genes, showed the broadest inhibitory activity. Conversely, the EMKD 24/1 strain which contain only the Ent B gene only inhibited the EA5 strain.

Implications

  • The E. mundtii found were tolerant to low pH levels (3) and oxgall/bile.They also did not induce hemolysis, gelatinase or DNase and were mostly susceptible to clinical antibiotics.
  • The aforementioned properties make these strains potentially suitable for application in a scenario where a bacterium strain with broad antimicrobial properties is needed.
  • Finally, the researchers suggest that the strain with the widest antimicrobial spectrum could have practical benefits, such as optimizing the host microbiota, which can contribute to maintaining the health status of the animal.

Cite This Article

APA
Focková V, Styková E, Simonová MP, Maďar M, Kačírová J, Lauková A. (2022). Horses as a source of bioactive fecal strains Enterococcus mundtii. Vet Res Commun, 46(3), 739-747. https://doi.org/10.1007/s11259-022-09893-9

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 46
Issue: 3
Pages: 739-747

Researcher Affiliations

Focková, V
  • Centre of Biosciences of the Slovak Academy of Sciences, v.v.i. Institute of Animal Physiology, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Styková, E
  • University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia.
Simonová, M Pogány
  • Centre of Biosciences of the Slovak Academy of Sciences, v.v.i. Institute of Animal Physiology, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Maďar, M
  • University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia.
Kačírová, J
  • University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia.
Lauková, A
  • Centre of Biosciences of the Slovak Academy of Sciences, v.v.i. Institute of Animal Physiology, Šoltésovej 4-6, 040 01, Košice, Slovakia. laukova@saske.sk.

MeSH Terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Bacteria / genetics
  • Bacteriocins / genetics
  • Enterococcus
  • Feces
  • Female
  • Horses
  • Male
  • RNA, Ribosomal, 16S / genetics

Grant Funding

  • SK-PL2019-2022 / SAS

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Citations

This article has been cited 1 times.
  1. Achache W, Mege JL, Fellag M, Drancourt M. The Enterococcus secretome inhibits the growth of Mycobacterium tuberculosis complex mycobacteria.. Access Microbiol 2023;5(6).
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