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Clinical oral implants research2009; 20(1); 17-23; doi: 10.1111/j.1600-0501.2008.01608.x

Human osteoclast formation and activity on an equine spongy bone substitute.

Abstract: The aim of the present study was to evaluate the in vitro formation and activity of human osteoclasts (OCLs) generated on a new type of xenograft for bone substitution, an equine spongy bone. Methods: Peripheral blood mononuclear cells from healthy volunteers were used to generate OCLs in vitro in the presence of macrophage colony stimulating factor (M-CSF) and receptor activator of NF-kappaB ligand (RANKL) on bovine bone slices (positive control) and equine spongy bone. Morphological and biochemical methods were used to assess OCLs formation and activity. Results: Cells generated after 21 days of culture on equine spongy bone showed similar morphology to those on the positive control and displayed typical OCL markers and features, indicating that this material supported OCL formation. Moreover, these cells were functionally active on equine spongy bone with statistically significant differences compared with the control in the release of tartrate-resistant acid phosphatase (TRAcP5b) at days 14 and 21 of culture. With regard to the resorption, on equine bone, OCLs formed smaller discontinuous island-like lacunae rather than the typical lobulated, tracking resorption lacunae observed on the control. Conclusions: This study enables clinicians to tailor the usage of equine spongy bone and presents a model, which can be applied to the preclinical assessment of bone substitute material's resorbability and resorption rates.
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Publication Date: 2009-01-08 PubMed ID: 19126103DOI: 10.1111/j.1600-0501.2008.01608.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aims to investigate the formation and activity of human osteoclasts (cells that breakdown bone) on a new type of graft for bone replacement: equine spongy bone. The study reveals the potential use of equine spongy bone in medical practice, as it supported osteoclast formation similar to traditional bone grafts and offers a new model for preclinical studies on bone substitutes’ absorption rates.

Research Methodology

  • The study relied on peripheral blood mononuclear cells from healthy volunteers to produce osteoclasts in a laboratory.
  • These cells were nurtured with the use of macrophage colony stimulating factor (M-CSF) and receptor activator of NF-kappaB ligand (RANKL).
  • The researchers then introduced the cells to bovine bone slices (as a positive control) and the equine spongy bone for observation.
  • Using morphological and biochemical methods, they measured the formation and activity of the osteoclasts.

Research Findings

  • A comparison of cells created on the equine spongy bone and those on the control bovine counterpart showed similar morphology and markers, pointing to the equine material’s capacity to support osteoclast development.
  • The study also discovered that the cells were functionally active on equine spongy bone, with significant differential in the release of tartrate-resistant acid phosphatase (a type of enzyme) at 14 and 21 days of culture compared to the control.
  • The osteoclasts, however, formed smaller and discontinuous island-like lacunae on the equine bone, a stark contrast to the typical lobulated, tracking resorption lacunae observed on the control.

Research Conclusion

  • The research suggests the potential of equine spongy bone in clinical use as a graft for bone replacement, as it demonstrated capability to support osteoclast formation similar to bovine bone slices.
  • Overall, the discovery offers clinicians a novel model with which to adapt the use of equine spongy bone and carries a significance for the preclinical evaluation of the resorbability and resorption rates of bone substitute materials.

Cite This Article

APA
Perrotti V, Nicholls BM, Piattelli A. (2009). Human osteoclast formation and activity on an equine spongy bone substitute. Clin Oral Implants Res, 20(1), 17-23. https://doi.org/10.1111/j.1600-0501.2008.01608.x

Publication

Clinical oral implants research
ISSN: 1600-0501
NlmUniqueID: 9105713
Country: Denmark
Language: English
Volume: 20
Issue: 1
Pages: 17-23

Researcher Affiliations

Perrotti, Vittoria
  • Dental School, University of Chieti-Pescara, Chieti, Italy. v.perrotti@unich.it
Nicholls, Brian M
    Piattelli, Adriano

      MeSH Terms

      • Acid Phosphatase / biosynthesis
      • Animals
      • Bone Resorption
      • Bone Substitutes
      • Bone Transplantation
      • Cattle
      • Cell Adhesion
      • Cell Differentiation
      • Cells, Cultured
      • Horses
      • Humans
      • Leukocytes, Mononuclear / physiology
      • Microscopy, Confocal
      • Osteoclasts / cytology
      • Osteoclasts / physiology
      • Transplantation, Heterologous

      Citations

      This article has been cited 15 times.
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