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Home / Equine Research Bank / J Vet Sci / Hyaluronic acid has chondroprotective and joint-preserving e...
Journal of veterinary science2019; 20(6); e67; doi: 10.4142/jvs.2019.20.e67

Hyaluronic acid has chondroprotective and joint-preserving effects on LPS-induced synovitis in horses.

Abstract: The intra-articular use of hyaluronic acid (HA) for the treatment of synovitis and osteoarthritis is still controversial. As a consequence, corticosteroids remain the most frequently employed therapeutic agents, despite their potential systemic and local deleterious effects. This study examined the anti-inflammatory, antioxidant, and chondroprotective activities of low and high molecular weight hyaluronic acid (LMW-HA and HMW-HA) on lipopolysaccharide (LPS)-induced synovitis in horses compared to triamcinolone acetonide (TA). LPS was injected in the metacarpophalangeal joints, which were treated intra-articularly with either TA (as control) or LMW-HA or HMW-HA. Joint clinical evaluation and synovial fluid (SF) analysis were performed at 0, 8, 24, and 48 h. The white blood cell counts (WBC), prostaglandin E2 (PGE2), interleukin (IL)-1, IL-6, IL-10, tumor necrosis factor-α, chondroitin sulfate (CS) and HA concentrations, oxidative burst, and HA molecular weights were measured. TA reduced the lameness, swelling, and PGE2 release but increased the SF CS concentrations enormously at 24h and 48h, and decreased the SF HA modal molecular weight. These results indicate the breakdown of articular cartilage aggrecan and SF HA. In contrast, LMW-HA and HMW-HA were less effective in reducing the inflammation symptoms, but preserved the joints because only a modest increase in CS occurred at 24 h, decreasing at 48 h, and the SF HA was maintained. The HA-treatment also had anti-inflammatory actions, and LMW-HA was the most effective in reducing the release of cytokine. In summary, the HA treatment inhibited efficiently the digestion of cartilage proteoglycans and SF HA breakdown.
© 2019 The Korean Society of Veterinary Science.
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Publication Date: 2019-11-28 PubMed ID: 31775194PubMed Central: PMC6883190DOI: 10.4142/jvs.2019.20.e67Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the potential anti-inflammatory, antioxidant, and cartilage-protecting effects of hyaluronic acid (HA) in treating synovitis (joint inflammation), comparing its effectiveness to a commonly used treatment, triamcinolone acetonide (TA).

Introduction

  • The research involved an analysis of the impacts of low and high molecular weight hyaluronic acid (LMW-HA and HMW-HA) on synovitis in horses—a condition induced by lipopolysaccharide (LPS).
  • The study’s motivation lies in the observed controversy surrounding the use of HA in treating synovitis and osteoarthritis, despite corticosteroids like TA being frequently used but associated with potential harmful effects.

Methods

  • The researchers injected LPS in the metacarpophalangeal joints of horses, followed by an inter-articular treatment with either TA, LMW-HA, or HMW-HA.
  • Joint evaluations and synovial fluid (SF) analyses were then conducted at 0, 8, 24, and 48 hours after treatment.
  • They measured multiple factors within the SF including white blood cell counts, levels of various interleukins, tumor necrosis factor-α, prostaglandin E2 (PGE2), HA and chondroitin sulfate (CS) concentrations, and HA molecular weights.

Findings

  • For the control group treated with TA, the treatment effectively reduced lameness, swelling, and PGE2 release, but resulted in a significant increase in SF CS concentrations at the 24 and 48-hour marks. It also resulted in a decrease in SF HA modal molecular weight, indicating a breakdown of articular cartilage aggrecan and SF HA.
  • In contrast, the horses treated with LMW-HA and HMW-HA evidenced a more modest increase in CS at 24 hours, followed by a decrease at 48 hours. SF HA was maintained, indicating better preservation of the joints.
  • Additionally, LMW-HA was observed to be most effective in reducing the release of cytokine, thus exhibiting anti-inflammatory behaviors.
  • Overall, the results suggest that HA treatment can efficiently inhibit the digestion of cartilage proteoglycans and prevent the breakdown of SF HA.

Conclusion

  • The study concludes that compared to traditional treatments like TA, both types of hyaluronic acid (LMW-HA and HMW-HA) can have beneficial anti-inflammatory, antioxidant, and chondroprotective effects for treating joint inflammation.

Cite This Article

APA
Neuenschwander HM, Moreira JJ, Vendruscolo CP, Fülber J, Seidel SRT, Michelacci YM, Baccarin RYA. (2019). Hyaluronic acid has chondroprotective and joint-preserving effects on LPS-induced synovitis in horses. J Vet Sci, 20(6), e67. https://doi.org/10.4142/jvs.2019.20.e67

Publication

Journal of veterinary science
ISSN: 1976-555X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 20
Issue: 6
Pages: e67
PII: e67

Researcher Affiliations

Neuenschwander, Henrique M
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP 05508-270, Brazil.
Moreira, Juliana J
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP 05508-270, Brazil.
Vendruscolo, Cynthia P
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP 05508-270, Brazil.
Fülber, Joice
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP 05508-270, Brazil.
Seidel, Sarah R T
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP 05508-270, Brazil.
Michelacci, Yara M
  • Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04044-020, Brazil.
Baccarin, Raquel Y A
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP 05508-270, Brazil. baccarin@usp.br.

MeSH Terms

  • Animals
  • Horse Diseases / chemically induced
  • Horse Diseases / drug therapy
  • Horses
  • Hyaluronic Acid / pharmacology
  • Injections, Intra-Articular / veterinary
  • Lipopolysaccharides / administration & dosage
  • Male
  • Random Allocation
  • Synovial Fluid / drug effects
  • Synovitis / chemically induced
  • Synovitis / drug therapy
  • Synovitis / veterinary
  • Viscosupplements / pharmacology

Grant Funding

  • #14/13065-6 / Fundau00e7u00e3o de Amparo u00e0 Pesquisa do Estado de Su00e3o Paulo
  • #2010/16022-5 / Fundau00e7u00e3o de Amparo u00e0 Pesquisa do Estado de Su00e3o Paulo
  • #2013/07109-8 / Fundau00e7u00e3o de Amparo u00e0 Pesquisa do Estado de Su00e3o Paulo
  • #301326/2013-4 / Conselho Nacional de Desenvolvimento Cientu00edfico e Tecnolu00f3gico
  • #300830/2016-5 / Conselho Nacional de Desenvolvimento Cientu00edfico e Tecnolu00f3gico
  • 001 / Coordenau00e7u00e3o de Aperfeiu00e7oamento de Pessoal de Nu00edvel Superior

Conflict of Interest Statement

The authors declare no conflicts of interest.

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Citations

This article has been cited 11 times.
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