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Home / Equine Research Bank / Front Vet Sci / Identification of Multiple Blastocystis Subtypes in Domestic...
Frontiers in veterinary science2021; 8; 732129; doi: 10.3389/fvets.2021.732129

Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing.

Abstract: is frequently reported in fecal samples from animals and humans worldwide, and a variety of subtypes (STs) have been observed in wild and domestic animals. In Colombia, few studies have focused on the transmission dynamics and epidemiological importance of in animals. In this study, we characterized the frequency and subtypes of in fecal samples of domestic animals including pigs, minipigs, cows, dogs, horses, goats, sheep, and llama from three departments of Colombia. Of the 118 fecal samples included in this study 81.4% ( = 96) were positive for using a PCR that amplifies a fragment of the small subunit ribosomal RNA ( rRNA) gene. PCR positive samples were sequenced by next generation amplicon sequencing (NGS) to determine subtypes. Eleven subtypes were detected, ten previously reported, ST5 (50.7%), ST10 (47.8%), ST25 (34.3%), ST26 (29.8%), ST21 (22.4%), ST23 (22.4%), ST1 (17.9%), ST14 (16.4%), ST24 (14.9%), ST3 (7.5%), and a novel subtype, named ST32 (3.0%). Mixed infection and/or intra -subtype variations were identified in most of the samples. Novel ST32 was observed in two samples from a goat and a cow. To support novel subtype designation, a MinION based sequencing strategy was used to generate the full-length of the rRNA gene. Comparison of full-length nucleotide sequences with those from current valid subtypes supported the designation of ST32. This is the first study in Colombia using NGS to molecularly characterize subtypes of in farm animals. A great diversity of subtypes was observed in domestic animals including subtypes previously identified in humans. Additionally, subtype overlap between the different hosts examined in this study were observed. These findings highlight the presence of subtypes with zoonotic potential in farm animals indicating that farm animals could play a role in transmission to humans.
Copyright © 2021 Higuera, Herrera, Jimenez, García-Corredor, Pulido-Medellín, Bulla-Castañeda, Pinilla, Moreno-Pérez, Maloney, Santín and Ramírez.
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Publication Date: 2021-08-24 PubMed ID: 34504891PubMed Central: PMC8421793DOI: 10.3389/fvets.2021.732129Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study centers around identifying different subtypes of Blastocystis, a group of single-celled organisms, in domestic animals based in Colombia using an advanced genetic sequencing technique.

Overview of the Study

  • In this research, the scientists examined fecal samples from a variety of domestic animals in Colombia—pigs, minipigs, cows, dogs, horses, goats, sheep, and llama.
  • The purpose was to determine the presence and types (subtypes) of Blastocystis, which are commonly found in animals and humans, and have various subtypes.
  • Out of 118 fecal samples, 81.4% (or 96 samples) tested positive for Blastocystis. This was done using a polymerase chain reaction (PCR) technique that amplifies a part of the small subunit ribosomal RNA (rRNA) gene of the Blastocystis.

Amplicon-Based Next Generation Sequencing (NGS)

  • The researchers used next generation amplicon sequencing (NGS), an advanced genetic sequencing technique, on the PCR positive samples to identify the subtypes of Blastocystis present.
  • NGS is a high-throughput methodology that allows many different genetic sequences to be read simultaneously, making it a faster and more efficient way of sequencing genomes.

Findings and Implications

  • The scientists were able to identify eleven subtypes of Blastocystis, ten of which were previously reported, along with a new subtype named ST32.
  • The most common subtypes found were ST5 (50.7%) and ST10 (47.8%), followed by ST25 (34.3%) and ST26 (29.8%).
  • The new subtype, ST32, was observed in a goat and a cow. The presence of this new subtype was confirmed by generating the full-length of the Blastocystis rRNA gene through a sequencing strategy.
  • The study reveals a large diversity of Blastocystis subtypes in domestic animals, including subtypes earlier found in humans. This finding emphasizes how domestic animals could contribute to transmitting Blastocystis to humans.

Cite This Article

APA
Higuera A, Herrera G, Jimenez P, García-Corredor D, Pulido-Medellín M, Bulla-Castañeda DM, Pinilla JC, Moreno-Pérez DA, Maloney JG, Santín M, Ramírez JD. (2021). Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing. Front Vet Sci, 8, 732129. https://doi.org/10.3389/fvets.2021.732129

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 8
Pages: 732129
PII: 732129

Researcher Affiliations

Higuera, Adriana
  • Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.
Herrera, Giovanny
  • Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.
Jimenez, Paula
  • Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.
García-Corredor, Diego
  • Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.
Pulido-Medellín, Martin
  • Grupo de Investigación en Medicina Veterinaria y Zootecnia, Facultad de Ciencias Agropecuarias, Universidad Pedagógica y Tecnológica de Colombia, Bogotá, Colombia.
Bulla-Castañeda, Diana M
  • Grupo de Investigación en Medicina Veterinaria y Zootecnia, Facultad de Ciencias Agropecuarias, Universidad Pedagógica y Tecnológica de Colombia, Bogotá, Colombia.
Pinilla, Juan Carlos
  • Grupo de Investigación en Ciencias Agropecuarias, Facultad de Ciencias Exactas, Naturales y Agropecuarias, Universidad de Santander, Bucaramanga, Colombia.
Moreno-Pérez, Darwin A
  • Universidad de Ciencias Aplicadas y Ambientales, Bogotá, Colombia.
Maloney, Jenny G
  • Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center (BARC), United States Department of Agriculture (USDA-ARS), Beltsville, MD, United States.
Santín, Mónica
  • Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center (BARC), United States Department of Agriculture (USDA-ARS), Beltsville, MD, United States.
Ramírez, Juan David
  • Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

This article includes 90 references
  1. Alfellani MA, Taner-Mulla D, Jacob AS, Imeede CA, Yoshikawa H, Stensvold CR, Clark CG. Genetic diversity of blastocystis in livestock and zoo animals.. Protist 2013 Jul;164(4):497-509.
    doi: 10.1016/j.protis.2013.05.003pubmed: 23770574google scholar: lookup
  2. Villalobos G, Orozco-Mosqueda GE, Lopez-Perez M, Lopez-Escamilla E, Córdoba-Aguilar A, Rangel-Gamboa L, Olivo-Diaz A, Romero-Valdovinos M, Maravilla P, Martinez-Hernandez F. Suitability of internal transcribed spacers (ITS) as markers for the population genetic structure of Blastocystis spp.. Parasit Vectors 2014 Oct 3;7:461.
    doi: 10.1186/s13071-014-0461-2pmc: PMC4203911pubmed: 25274498google scholar: lookup
  3. Stensvold CR, Christiansen DB, Olsen KE, Nielsen HV. Blastocystis sp. subtype 4 is common in Danish Blastocystis-positive patients presenting with acute diarrhea.. Am J Trop Med Hyg 2011 Jun;84(6):883-5.
    doi: 10.4269/ajtmh.2011.11-0005pmc: PMC3110361pubmed: 21633023google scholar: lookup
  4. El Safadi D, Gaayeb L, Meloni D, Cian A, Poirier P, Wawrzyniak I, Delbac F, Dabboussi F, Delhaes L, Seck M, Hamze M, Riveau G, Viscogliosi E. Children of Senegal River Basin show the highest prevalence of Blastocystis sp. ever observed worldwide.. BMC Infect Dis 2014 Mar 25;14:164.
    doi: 10.1186/1471-2334-14-164pmc: PMC3987649pubmed: 24666632google scholar: lookup
  5. Osman M, El Safadi D, Cian A, Benamrouz S, Nourrisson C, Poirier P, Pereira B, Razakandrainibe R, Pinon A, Lambert C, Wawrzyniak I, Dabboussi F, Delbac F, Favennec L, Hamze M, Viscogliosi E, Certad G. Prevalence and Risk Factors for Intestinal Protozoan Infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among Schoolchildren in Tripoli, Lebanon.. PLoS Negl Trop Dis 2016 Mar;10(3):e0004496.
    doi: 10.1371/journal.pntd.0004496pmc: PMC4790957pubmed: 26974335google scholar: lookup
  6. Stensvold CR, Nielsen HV, Mølbak K, Smith HV. Pursuing the clinical significance of Blastocystis--diagnostic limitations.. Trends Parasitol 2009 Jan;25(1):23-9.
    doi: 10.1016/j.pt.2008.09.010pubmed: 19013108google scholar: lookup
  7. Parkar U, Traub RJ, Vitali S, Elliot A, Levecke B, Robertson I, Geurden T, Steele J, Drake B, Thompson RC. Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers.. Vet Parasitol 2010 Apr 19;169(1-2):8-17.
    doi: 10.1016/j.vetpar.2009.12.032pubmed: 20089360google scholar: lookup
  8. Cian A, El Safadi D, Osman M, Moriniere R, Gantois N, Benamrouz-Vanneste S, Delgado-Viscogliosi P, Guyot K, Li LL, Monchy S, Noël C, Poirier P, Nourrisson C, Wawrzyniak I, Delbac F, Bosc S, Chabé M, Petit T, Certad G, Viscogliosi E. Molecular Epidemiology of Blastocystis sp. in Various Animal Groups from Two French Zoos and Evaluation of Potential Zoonotic Risk.. PLoS One 2017;12(1):e0169659.
    doi: 10.1371/journal.pone.0169659pmc: PMC5217969pubmed: 28060901google scholar: lookup
  9. Ning CQ, Hu ZH, Chen JH, Ai L, Tian LG. Epidemiology of Blastocystis infection from 1990 to 2019 in China.. Infect Dis Poverty 2020 Dec 30;9(1):168.
    doi: 10.1186/s40249-020-00779-zpmc: PMC7772921pubmed: 33380335google scholar: lookup
  10. Rauff-Adedotun AA, Mohd Zain SN, Farah Haziqah MT. Current status of Blastocystis sp. in animals from Southeast Asia: a review.. Parasitol Res 2020 Nov;119(11):3559-3570.
    doi: 10.1007/s00436-020-06828-8pmc: PMC7502158pubmed: 32951145google scholar: lookup
  11. Rajah Salim H, Suresh Kumar G, Vellayan S, Mak JW, Khairul Anuar A, Init I, Vennila GD, Saminathan R, Ramakrishnan K. Blastocystis in animal handlers.. Parasitol Res 1999 Dec;85(12):1032-3.
    doi: 10.1007/s004360050677pubmed: 10599928google scholar: lookup
  12. Hublin JSY, Maloney JG, Santin M. Blastocystis in domesticated and wild mammals and birds.. Res Vet Sci 2021 Mar;135:260-282.
    doi: 10.1016/j.rvsc.2020.09.031pubmed: 33046256google scholar: lookup
  13. Stensvold CR, Clark CG. Pre-empting Pandora's Box: Blastocystis Subtypes Revisited.. Trends Parasitol 2020 Mar;36(3):229-232.
    doi: 10.1016/j.pt.2019.12.009pubmed: 32001133google scholar: lookup
  14. Maloney JG, Santin M. Mind the Gap: New Full-Length Sequences of Blastocystis Subtypes Generated via Oxford Nanopore Minion Sequencing Allow for Comparisons between Full-Length and Partial Sequences of the Small Subunit of the Ribosomal RNA Gene.. Microorganisms 2021 May 5;9(5).
    doi: 10.3390/microorganisms9050997pmc: PMC8147991pubmed: 34063045google scholar: lookup
  15. Jiménez PA, Jaimes JE, Ramírez JD. A summary of Blastocystis subtypes in North and South America.. Parasit Vectors 2019 Jul 29;12(1):376.
    doi: 10.1186/s13071-019-3641-2pmc: PMC6664531pubmed: 31358042google scholar: lookup
  16. Maloney JG, Jang Y, Molokin A, George NS, Santin M. Wide Genetic Diversity of Blastocystis in White-Tailed Deer (Odocoileus virginianus) from Maryland, USA.. Microorganisms 2021 Jun 21;9(6).
    doi: 10.3390/microorganisms9061343pmc: PMC8233720pubmed: 34205799google scholar: lookup
  17. Ramírez JD, Sánchez A, Hernández C, Flórez C, Bernal MC, Giraldo JC, Reyes P, López MC, García L, Cooper PJ, Vicuña Y, Mongi F, Casero RD. Geographic distribution of human Blastocystis subtypes in South America.. Infect Genet Evol 2016 Jul;41:32-35.
    doi: 10.1016/j.meegid.2016.03.017pubmed: 27034056google scholar: lookup
  18. Stensvold CR, Alfellani MA, Nørskov-Lauritsen S, Prip K, Victory EL, Maddox C, Nielsen HV, Clark CG. Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype.. Int J Parasitol 2009 Mar;39(4):473-9.
    doi: 10.1016/j.ijpara.2008.07.006pubmed: 18755193google scholar: lookup
  19. Andersen LO, Stensvold CR. Blastocystis in Health and Disease: Are We Moving from a Clinical to a Public Health Perspective?. J Clin Microbiol 2016 Mar;54(3):524-8.
    doi: 10.1128/JCM.02520-15pmc: PMC4767957pubmed: 26677249google scholar: lookup
  20. Santín M, Gómez-Muñoz MT, Solano-Aguilar G, Fayer R. Development of a new PCR protocol to detect and subtype Blastocystis spp. from humans and animals.. Parasitol Res 2011 Jul;109(1):205-12.
    doi: 10.1007/s00436-010-2244-9pubmed: 21210149google scholar: lookup
  21. Suwanti LT, Susana Y, Hastutiek P, Suprihati E, Lastuti NDR. Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia.. Vet World 2020 Feb;13(2):231-237.
    doi: 10.14202/vetworld.2020.231-237pmc: PMC7096301pubmed: 32255963google scholar: lookup
  22. Song JK, Yin YL, Yuan YJ, Tang H, Ren GJ, Zhang HJ, Li ZX, Zhang YM, Zhao GH. First genotyping of Blastocystis sp. in dairy, meat, and cashmere goats in northwestern China.. Acta Trop 2017 Dec;176:277-282.
    doi: 10.1016/j.actatropica.2017.08.028pubmed: 28864325google scholar: lookup
  23. Fayer R, Elsasser T, Gould R, Solano G, Urban J Jr, Santin M. Blastocystis tropism in the pig intestine.. Parasitol Res 2014 Apr;113(4):1465-72.
    doi: 10.1007/s00436-014-3787-ypubmed: 24535732google scholar: lookup
  24. Wang W, Owen H, Traub RJ, Cuttell L, Inpankaew T, Bielefeldt-Ohmann H. Molecular epidemiology of Blastocystis in pigs and their in-contact humans in Southeast Queensland, Australia, and Cambodia.. Vet Parasitol 2014 Jul 14;203(3-4):264-9.
    doi: 10.1016/j.vetpar.2014.04.006pubmed: 24785292google scholar: lookup
  25. Moura RGF, Oliveira-Silva MB, Pedrosa AL, Nascentes GAN, Cabrine-Santos M. Occurrence of Blastocystis spp. in domestic animals in Triângulo Mineiro area of Brazil.. Rev Soc Bras Med Trop 2018 Mar-Apr;51(2):240-243.
    doi: 10.1590/0037-8682-0484-2016pubmed: 29768563google scholar: lookup
  26. Ramírez JD, Sánchez LV, Bautista DC, Corredor AF, Flórez AC, Stensvold CR. Blastocystis subtypes detected in humans and animals from Colombia.. Infect Genet Evol 2014 Mar;22:223-8.
    doi: 10.1016/j.meegid.2013.07.020pubmed: 23886615google scholar: lookup
  27. Greige S, El Safadi D, Bécu N, Gantois N, Pereira B, Chabé M, Benamrouz-Vanneste S, Certad G, El Hage R, Chemaly M, Hamze M, Viscogliosi E. Prevalence and subtype distribution of Blastocystis sp. isolates from poultry in Lebanon and evidence of zoonotic potential.. Parasit Vectors 2018 Jul 4;11(1):389.
    doi: 10.1186/s13071-018-2975-5pmc: PMC6030734pubmed: 29973261google scholar: lookup
  28. Maloney JG, da Cunha MJR, Molokin A, Cury MC, Santin M. Next-generation sequencing reveals wide genetic diversity of Blastocystis subtypes in chickens including potentially zoonotic subtypes.. Parasitol Res 2021 Jun;120(6):2219-2231.
    doi: 10.1007/s00436-021-07170-3pubmed: 33904983google scholar: lookup
  29. Maloney JG, Lombard JE, Urie NJ, Shivley CB, Santin M. Zoonotic and genetically diverse subtypes of Blastocystis in US pre-weaned dairy heifer calves.. Parasitol Res 2019 Feb;118(2):575-582.
    doi: 10.1007/s00436-018-6149-3pubmed: 30483890google scholar: lookup
  30. Ruaux CG, Stang BV. Prevalence of blastocystis in shelter-resident and client-owned companion animals in the US Pacific Northwest.. PLoS One 2014;9(9):e107496.
    doi: 10.1371/journal.pone.0107496pmc: PMC4166454pubmed: 25226285google scholar: lookup
  31. Martinez-Hernandez F, Martinez-Ibarra JA, Lopez-Escamilla E, Villanueva-Garcia C, Muñoz-Garcia CI, Rendon-Franco E, Maravilla P, Villalobos G. Molecular genotyping of Blastocystis spp. in wild mammals from Mexico.. Parasitol Res 2020 Jan;119(1):97-104.
    doi: 10.1007/s00436-019-06530-4pubmed: 31735993google scholar: lookup
  32. David ÉB, Guimarães S, de Oliveira AP, Goulart de Oliveira-Sequeira TC, Nogueira Bittencourt G, Moraes Nardi AR, Martins Ribolla PE, Bueno Franco RM, Branco N, Tosini F, Bella A, Pozio E, Cacciò SM. Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil.. Parasit Vectors 2015 Feb 15;8:103.
    doi: 10.1186/s13071-015-0714-8pmc: PMC4335703pubmed: 25889093google scholar: lookup
  33. Maloney JG, Molokin A, da Cunha MJR, Cury MC, Santin M. Blastocystis subtype distribution in domestic and captive wild bird species from Brazil using next generation amplicon sequencing.. Parasite Epidemiol Control 2020 May;9:e00138.
    doi: 10.1016/j.parepi.2020.e00138pmc: PMC6995250pubmed: 32021915google scholar: lookup
  34. Helenbrook WD, Whipps CM. Molecular Characterization of Blastocystis in Captive and Free-Ranging New World Primates, Platyrrhini.. Acta Parasitol 2021 Dec;66(4):1267-1273.
    doi: 10.1007/s11686-021-00397-1pubmed: 33914238google scholar: lookup
  35. Helenbrook WD, Shields WM, Whipps CM. Characterization of Blastocystis species infection in humans and mantled howler monkeys, Alouatta palliata aequatorialis, living in close proximity to one another.. Parasitol Res 2015 Jul;114(7):2517-25.
    doi: 10.1007/s00436-015-4451-xpubmed: 25859926google scholar: lookup
  36. Villamizar X, Higuera A, Herrera G, Vasquez-A LR, Buitron L, Muñoz LM, Gonzalez-C FE, Lopez MC, Giraldo JC, Ramírez JD. Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: a cross-sectional study.. BMC Infect Dis 2019 Feb 26;19(1):190.
    doi: 10.1186/s12879-019-3810-0pmc: PMC6390308pubmed: 30808303google scholar: lookup
  37. Scanlan PD, Stensvold CR, Cotter PD. Development and Application of a Blastocystis Subtype-Specific PCR Assay Reveals that Mixed-Subtype Infections Are Common in a Healthy Human Population.. Appl Environ Microbiol 2015 Jun 15;81(12):4071-6.
    doi: 10.1128/AEM.00520-15pmc: PMC4524157pubmed: 25841010google scholar: lookup
  38. Clark CG, van der Giezen M, Alfellani MA, Stensvold CR. Recent developments in Blastocystis research.. Adv Parasitol 2013;82:1-32.
    doi: 10.1016/B978-0-12-407706-5.00001-0pubmed: 23548084google scholar: lookup
  39. Meloni D, Poirier P, Mantini C, Noël C, Gantois N, Wawrzyniak I, Delbac F, Chabé M, Delhaes L, Dei-Cas E, Fiori PL, El Alaoui H, Viscogliosi E. Mixed human intra- and inter-subtype infections with the parasite Blastocystis sp.. Parasitol Int 2012 Dec;61(4):719-22.
    doi: 10.1016/j.parint.2012.05.012pubmed: 22659011google scholar: lookup
  40. Rojas-Velázquez L, Maloney JG, Molokin A, Morán P, Serrano-Vázquez A, González E, Pérez-Juárez H, Ximénez C, Santin M. Use of next-generation amplicon sequencing to study Blastocystis genetic diversity in a rural human population from Mexico.. Parasit Vectors 2019 Nov 27;12(1):566.
    doi: 10.1186/s13071-019-3814-zpmc: PMC6882168pubmed: 31775832google scholar: lookup
  41. Calero-Bernal R, Santín M, Maloney JG, Martín-Pérez M, Habela MA, Fernández-García JL, Figueiredo A, Nájera F, Palacios MJ, Mateo M, Balseiro A, Barral M, Lima-Barberoi JF, Köster PC, Carmena D. Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain.. J Eukaryot Microbiol 2020 Mar;67(2):273-278.
    doi: 10.1111/jeu.12772pubmed: 31691450google scholar: lookup
  42. Vega L, Herrera G, Muñoz M, Patarroyo MA, Maloney JG, Santín M, Ramírez JD. Gut microbiota profiles in diarrheic patients with co-occurrence of Clostridioides difficile and Blastocystis.. PLoS One 2021;16(3):e0248185.
    doi: 10.1371/journal.pone.0248185pmc: PMC7963057pubmed: 33725006google scholar: lookup
  43. Higuera A, Villamizar X, Herrera G, Giraldo JC, Vasquez-A LR, Urbano P, Villalobos O, Tovar C, Ramírez JD. Molecular detection and genotyping of intestinal protozoa from different biogeographical regions of Colombia.. PeerJ 2020;8:e8554.
    doi: 10.7717/peerj.8554pmc: PMC7067185pubmed: 32195042google scholar: lookup
  44. Stensvold CR, Ahmed UN, Andersen LO, Nielsen HV. Development and evaluation of a genus-specific, probe-based, internal-process-controlled real-time PCR assay for sensitive and specific detection of Blastocystis spp.. J Clin Microbiol 2012 Jun;50(6):1847-51.
    doi: 10.1128/JCM.00007-12pmc: PMC3372105pubmed: 22422846google scholar: lookup
  45. Maloney JG, Molokin A, Santin M. Next generation amplicon sequencing improves detection of Blastocystis mixed subtype infections.. Infect Genet Evol 2019 Sep;73:119-125.
    doi: 10.1016/j.meegid.2019.04.013pubmed: 31026606google scholar: lookup
  46. Rognes T, Flouri T, Nichols B, Quince C, Mahé F. VSEARCH: a versatile open source tool for metagenomics.. PeerJ 2016;4:e2584.
    doi: 10.7717/peerj.2584pmc: PMC5075697pubmed: 27781170google scholar: lookup
  47. Maloney JG, Molokin A, Santin M. Use of Oxford Nanopore MinION to generate full-length sequences of the Blastocystis small subunit (SSU) rRNA gene.. Parasit Vectors 2020 Nov 25;13(1):595.
    doi: 10.1186/s13071-020-04484-6pmc: PMC7687777pubmed: 33239096google scholar: lookup
  48. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.. J Mol Evol 1980 Dec;16(2):111-20.
    doi: 10.1007/BF01731581pubmed: 7463489google scholar: lookup
  49. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.. Mol Biol Evol 2018 Jun 1;35(6):1547-1549.
    doi: 10.1093/molbev/msy096pmc: PMC5967553pubmed: 29722887google scholar: lookup
  50. Jones MS, Whipps CM, Ganac RD, Hudson NR, Boorom K. Association of Blastocystis subtype 3 and 1 with patients from an Oregon community presenting with chronic gastrointestinal illness.. Parasitol Res 2009 Jan;104(2):341-5.
    doi: 10.1007/s00436-008-1198-7pubmed: 18923844google scholar: lookup
  51. Whipps CM, Boorom K, Bermudez LE, Kent ML. Molecular characterization of Blastocystis species in Oregon identifies multiple subtypes.. Parasitol Res 2010 Mar;106(4):827-32.
    doi: 10.1007/s00436-010-1739-8pubmed: 20127113google scholar: lookup
  52. Scanlan PD, Knight R, Song SJ, Ackermann G, Cotter PD. Prevalence and genetic diversity of Blastocystis in family units living in the United States.. Infect Genet Evol 2016 Nov;45:95-97.
    doi: 10.1016/j.meegid.2016.08.018pubmed: 27545648google scholar: lookup
  53. Nash AK, Auchtung TA, Wong MC, Smith DP, Gesell JR, Ross MC, Stewart CJ, Metcalf GA, Muzny DM, Gibbs RA, Ajami NJ, Petrosino JF. The gut mycobiome of the Human Microbiome Project healthy cohort.. Microbiome 2017 Nov 25;5(1):153.
    doi: 10.1186/s40168-017-0373-4pmc: PMC5702186pubmed: 29178920google scholar: lookup
  54. Casero RD, Mongi F, Sánchez A, Ramírez JD. Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation.. Acta Trop 2015 Aug;148:156-61.
    doi: 10.1016/j.actatropica.2015.05.004pubmed: 25976414google scholar: lookup
  55. Ramírez JD, Flórez C, Olivera M, Bernal MC, Giraldo JC. Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia.. PLoS One 2017;12(2):e0172586.
    doi: 10.1371/journal.pone.0172586pmc: PMC5319748pubmed: 28222192google scholar: lookup
  56. Rojas-Velázquez L, Morán P, Serrano-Vázquez A, Fernández LD, Pérez-Juárez H, Poot-Hernández AC, Portillo T, González E, Hernández E, Partida-Rodríguez O, Nieves-Ramírez ME, Magaña U, Torres J, Eguiarte LE, Piñero D, Ximénez C. Genetic Diversity and Distribution of Blastocystis Subtype 3 in Human Populations, with Special Reference to a Rural Population in Central Mexico.. Biomed Res Int 2018;2018:3916263.
    doi: 10.1155/2018/3916263pmc: PMC5878905pubmed: 29744356google scholar: lookup
  57. Melo GB, Malta FM, Maruta CW, Criado PR, Castilho VLP, Gonçalves EMDN, Espirito-Santo MCCD, Paula FM, Gryschek RCB. Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil.. Parasite Epidemiol Control 2019 Nov;7:e00124.
    doi: 10.1016/j.parepi.2019.e00124pmc: PMC6911935pubmed: 31872093google scholar: lookup
  58. Ascuña-Durand K, Salazar-Sánchez RS, Castillo-Neyra R, Ballón-Echegaray J. Relative Frequency of Blastocystis Subtypes 1, 2, and 3 in Urban and Periurban Human Populations of Arequipa, Peru.. Trop Med Infect Dis 2020 Nov 27;5(4).
    doi: 10.3390/tropicalmed5040178pmc: PMC7709661pubmed: 33261137google scholar: lookup
  59. Fayer R, Santin M, Macarisin D. Detection of concurrent infection of dairy cattle with Blastocystis, Cryptosporidium, Giardia, and Enterocytozoon by molecular and microscopic methods.. Parasitol Res 2012 Sep;111(3):1349-55.
    doi: 10.1007/s00436-012-2971-1pubmed: 22710524google scholar: lookup
  60. Angelici MC, Nardis C, Scarpelli R, Ade P. Blastocystis hominis transmission by non-potable water: a case report in Italy.. New Microbiol 2018 Apr;41(2):173-177.
    pubmed: 29498738
  61. Quílez J, Sánchez-Acedo C, Clavel A, Causapé AC. Occurrence of blastocystis sp. in cattle in Aragón, northeastern Spain.. Parasitol Res 1995;81(8):703-5.
    doi: 10.1007/BF00931851pubmed: 8570589google scholar: lookup
  62. Rivera WL, Tan MA. Molecular characterization of Blastocystis isolates in the Philippines by riboprinting.. Parasitol Res 2005 Jun;96(4):253-7.
    doi: 10.1007/s00436-005-1344-4pubmed: 15886995google scholar: lookup
  63. Valença Barbosa C, de Jesus Batista R, Pereira Igreja R, d'Avila Levy CM, Werneck de Macedo H, Carneiro Santos HL. Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil.. Parasit Vectors 2017 Oct 25;10(1):518.
    doi: 10.1186/s13071-017-2458-0pmc: PMC5657060pubmed: 29070053google scholar: lookup
  64. Wang W, Bielefeldt-Ohmann H, Traub RJ, Cuttell L, Owen H. Location and pathogenic potential of Blastocystis in the porcine intestine.. PLoS One 2014;9(8):e103962.
    doi: 10.1371/journal.pone.0103962pmc: PMC4122384pubmed: 25093578google scholar: lookup
  65. Wang J, Gong B, Yang F, Zhang W, Zheng Y, Liu A. Subtype distribution and genetic characterizations of Blastocystis in pigs, cattle, sheep and goats in northeastern China's Heilongjiang Province.. Infect Genet Evol 2018 Jan;57:171-176.
    doi: 10.1016/j.meegid.2017.11.026pubmed: 29196130google scholar: lookup
  66. AbuOdeh R, Ezzedine S, Madkour M, Stensvold CR, Samie A, Nasrallah G, AlAbsi E, ElBakri A. Molecular Subtyping of Blastocystis from Diverse Animals in the United Arab Emirates.. Protist 2019 Nov;170(5):125679.
    doi: 10.1016/j.protis.2019.125679pubmed: 31580985google scholar: lookup
  67. Li WC, Wang K, Gu Y. Occurrence of Blastocystis sp. and Pentatrichomonas hominis in sheep and goats in China.. Parasit Vectors 2018 Feb 17;11(1):93.
    doi: 10.1186/s13071-018-2671-5pmc: PMC5816562pubmed: 29454366google scholar: lookup
  68. Tan TC, Tan PC, Sharma R, Sugnaseelan S, Suresh KG. Genetic diversity of caprine Blastocystis from Peninsular Malaysia.. Parasitol Res 2013 Jan;112(1):85-9.
    doi: 10.1007/s00436-012-3107-3pubmed: 22961236google scholar: lookup
  69. Udonsom R, Prasertbun R, Mahittikorn A, Mori H, Changbunjong T, Komalamisra C, Pintong AR, Sukthana Y, Popruk S. Blastocystis infection and subtype distribution in humans, cattle, goats, and pigs in central and western Thailand.. Infect Genet Evol 2018 Nov;65:107-111.
    doi: 10.1016/j.meegid.2018.07.007pubmed: 30003970google scholar: lookup
  70. Stenzel DJ, Cassidy MF, Boreham PF. Morphology of Blastocystis sp. isolated from circus animals.. Int J Parasitol 1993 Aug;23(5):685-7.
    doi: 10.1016/0020-7519(93)90179-3pubmed: 8225774google scholar: lookup
  71. Thathaisong U, Worapong J, Mungthin M, Tan-Ariya P, Viputtigul K, Sudatis A, Noonai A, Leelayoova S. Blastocystis isolates from a pig and a horse are closely related to Blastocystis hominis.. J Clin Microbiol 2003 Mar;41(3):967-75.
    doi: 10.1128/JCM.41.3.967-975.2003pmc: PMC150316pubmed: 12624017google scholar: lookup
  72. Wang W, Cuttell L, Bielefeldt-Ohmann H, Inpankaew T, Owen H, Traub RJ. Diversity of Blastocystis subtypes in dogs in different geographical settings.. Parasit Vectors 2013 Jul 24;6:215.
    doi: 10.1186/1756-3305-6-215pmc: PMC3734043pubmed: 23883734google scholar: lookup
  73. Mohammadpour I, Bozorg-Ghalati F, Gazzonis AL, Manfredi MT, Motazedian MH, Mohammadpour N. First molecular subtyping and phylogeny of Blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern Iran.. Parasit Vectors 2020 Jul 22;13(1):365.
    doi: 10.1186/s13071-020-04225-9pmc: PMC7374852pubmed: 32698882google scholar: lookup
  74. Nagel R, Cuttell L, Stensvold CR, Mills PC, Bielefeldt-Ohmann H, Traub RJ. Blastocystis subtypes in symptomatic and asymptomatic family members and pets and response to therapy.. Intern Med J 2012 Nov;42(11):1187-95.
    doi: 10.1111/j.1445-5994.2011.02626.xpubmed: 22032439google scholar: lookup
  75. Li TS, Zou Y, Ma YT, Ma YY, Chen H, Liang XX, Cong W, Sun XL, Zhu XQ. Molecular characterization of Eimeria spp. and Blastocystis in rabbits in Shandong Province, China.. Parasitol Res 2020 May;119(5):1547-1551.
    doi: 10.1007/s00436-020-06652-0pubmed: 32198626google scholar: lookup
  76. Yoshikawa H, Abe N, Wu Z. PCR-based identification of zoonotic isolates of Blastocystis from mammals and birds.. Microbiology (Reading) 2004 May;150(Pt 5):1147-1151.
    doi: 10.1099/mic.0.26899-0pubmed: 15133074google scholar: lookup
  77. Parkar U, Traub RJ, Kumar S, Mungthin M, Vitali S, Leelayoova S, Morris K, Thompson RC. Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential.. Parasitology 2007 Mar;134(Pt 3):359-67.
    doi: 10.1017/S0031182006001582pubmed: 17052374google scholar: lookup
  78. Dogruman-Al F, Kustimur S, Yoshikawa H, Tuncer C, Simsek Z, Tanyuksel M, Araz E, Boorom K. Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara, Turkey.. Mem Inst Oswaldo Cruz 2009 Aug;104(5):724-7.
    doi: 10.1590/S0074-02762009000500011pubmed: 19820833google scholar: lookup
  79. Moosavi A, Haghighi A, Mojarad EN, Zayeri F, Alebouyeh M, Khazan H, Kazemi B, Zali MR. Genetic variability of Blastocystis sp. isolated from symptomatic and asymptomatic individuals in Iran.. Parasitol Res 2012 Dec;111(6):2311-5.
    doi: 10.1007/s00436-012-3085-5pubmed: 22948205google scholar: lookup
  80. Stensvold CR, Alfellani M, Clark CG. Levels of genetic diversity vary dramatically between Blastocystis subtypes.. Infect Genet Evol 2012 Mar;12(2):263-73.
    doi: 10.1016/j.meegid.2011.11.002pubmed: 22116021google scholar: lookup
  81. Alfellani MA, Stensvold CR, Vidal-Lapiedra A, Onuoha ES, Fagbenro-Beyioku AF, Clark CG. Variable geographic distribution of Blastocystis subtypes and its potential implications.. Acta Trop 2013 Apr;126(1):11-8.
    doi: 10.1016/j.actatropica.2012.12.011pubmed: 23290980google scholar: lookup
  82. Russini V, Di Filippo MM, Fanelli R, Polidori M, Berrilli F, Di Cave D, Novelletto A, Calderini P. Characterization of prevalence and genetic subtypes of Blastocystis sp. in wild and domestic Suidae of central Italy aided by amplicon NGS.. Vet Parasitol Reg Stud Reports 2020 Dec;22:100472.
    doi: 10.1016/j.vprsr.2020.100472pubmed: 33308752google scholar: lookup
  83. Morris A, Robinson G, Swain MT, Chalmers RM. Direct Sequencing of Cryptosporidium in Stool Samples for Public Health.. Front Public Health 2019;7:360.
    doi: 10.3389/fpubh.2019.00360pmc: PMC6917613pubmed: 31921734google scholar: lookup
  84. Samie A, Tanih NF, Seisa I, Seheri M, Mphahlele J, ElBakri A, Mbati P. Prevalence and genetic characterization of Giardia lamblia in relation to diarrhea in Limpopo and Gauteng provinces, South Africa.. Parasite Epidemiol Control 2020 May;9:e00140.
    doi: 10.1016/j.parepi.2020.e00140pmc: PMC7016452pubmed: 32083192google scholar: lookup
  85. Maiguashca Sánchez J, Sueto SOB, Schwabl P, Grijalva MJ, Llewellyn MS, Costales JA. Remarkable genetic diversity of Trypanosoma cruzi and Trypanosoma rangeli in two localities of southern Ecuador identified via deep sequencing of mini-exon gene amplicons.. Parasit Vectors 2020 May 14;13(1):252.
    doi: 10.1186/s13071-020-04079-1pmc: PMC7227245pubmed: 32410645google scholar: lookup
  86. Arias-Giraldo LM, Muñoz M, Hernández C, Herrera G, Velásquez-Ortiz N, Cantillo-Barraza O, Urbano P, Cuervo A, Ramírez JD. Identification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius and Triatoma using amplicon-based next-generation sequencing.. Parasit Vectors 2020 Aug 31;13(1):434.
    doi: 10.1186/s13071-020-04310-zpmc: PMC7457505pubmed: 32867816google scholar: lookup
  87. Early AM, Daniels RF, Farrell TM, Grimsby J, Volkman SK, Wirth DF, MacInnis BL, Neafsey DE. Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing.. Malar J 2019 Jul 1;18(1):219.
    doi: 10.1186/s12936-019-2856-1pmc: PMC6604269pubmed: 31262308google scholar: lookup
  88. Maloney JG, Molokin A, Santin M. Assessment of next generation amplicon sequencing of the beta-giardin gene for the detection of Giardia duodenalis assemblages and mixed infections.. Food Waterborne Parasitol 2020 Dec;21:e00098.
    doi: 10.1016/j.fawpar.2020.e00098pmc: PMC7691155pubmed: 33294649google scholar: lookup
  89. Greige S, El Safadi D, Khaled S, Gantois N, Baydoun M, Chemaly M, Benamrouz-Vanneste S, Chabé M, Osman M, Certad G, Hamze M, Viscogliosi E. First report on the prevalence and subtype distribution of Blastocystis sp. in dairy cattle in Lebanon and assessment of zoonotic transmission.. Acta Trop 2019 Jun;194:23-29.
    doi: 10.1016/j.actatropica.2019.02.013pubmed: 30878470google scholar: lookup
  90. Noël C, Dufernez F, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Ho LC, Singh M, Wintjens R, Sogin ML, Capron M, Pierce R, Zenner L, Viscogliosi E. Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis.. J Clin Microbiol 2005 Jan;43(1):348-55.
    doi: 10.1128/JCM.43.1.348-355.2005pmc: PMC540115pubmed: 15634993google scholar: lookup

Citations

This article has been cited 26 times.
  1. Santin M, Molokin A, Maloney JG. A longitudinal study of Blastocystis in dairy calves from birth through 24 months demonstrates dynamic shifts in infection rates and subtype prevalence and diversity by age.. Parasit Vectors 2023 Jun 2;16(1):177.
    doi: 10.1186/s13071-023-05795-0pubmed: 37264466google scholar: lookup
  2. McCain A, Gruneck L, Popluechai S, Tsaousis AD, Gentekaki E. Circulation and colonisation of Blastocystis subtypes in schoolchildren of various ethnicities in rural northern Thailand.. Epidemiol Infect 2023 Apr 27;151:e77.
    doi: 10.1017/S0950268823000596pubmed: 37185159google scholar: lookup
  3. Hernández PC, Maloney JG, Molokin A, George NS, Morales L, Chaparro-Olaya J, Santin M. Exploring Blastocystis genetic diversity in rural schoolchildren from Colombia using next-generation amplicon sequencing reveals significant associations between contact with animals and infection risk.. Parasitol Res 2023 Jul;122(7):1451-1462.
    doi: 10.1007/s00436-023-07841-3pubmed: 37156906google scholar: lookup
  4. Rondón S, Cavallero S, Link A, González C, D'Amelio S. Prevalence and Molecular Characterisation of Blastocystis sp. Infecting Free-Ranging Primates in Colombia.. Pathogens 2023 Apr 6;12(4).
    doi: 10.3390/pathogens12040569pubmed: 37111455google scholar: lookup
  5. Yu M, Yao Y, Xiao H, Xie M, Xiong Y, Yang S, Ni Q, Zhang M, Xu H. Extensive prevalence and significant genetic differentiation of Blastocystis in high- and low-altitude populations of wild rhesus macaques in China.. Parasit Vectors 2023 Mar 17;16(1):107.
    doi: 10.1186/s13071-023-05691-7pubmed: 36932438google scholar: lookup
  6. Meclat VYB, Ancheta KSB, Adao DEV, Rivera WL. Phylogenetic relationship of nonmammalian and avian Blastocystis isolates and conventional subtypes.. J Parasit Dis 2023 Mar;47(1):192-197.
    doi: 10.1007/s12639-022-01554-7pubmed: 36910318google scholar: lookup
  7. Zhang M, Zhang J, Sun J, Zhu S, Xu H, Li X, Zhang L, Li J. First identification of Blastocystis sp. subtypes in Rex rabbits in China.. Parasitol Res 2023 May;122(5):1079-1086.
    doi: 10.1007/s00436-023-07807-5pubmed: 36897380google scholar: lookup
  8. Higuera A, Salas-Leiva DE, Curtis B, Patiño LH, Zhao D, Jerlström-Hultqvist J, Dlutek M, Muñoz M, Roger AJ, Ramírez JD. Draft genomes of Blastocystis subtypes from human samples of Colombia.. Parasit Vectors 2023 Feb 2;16(1):52.
    doi: 10.1186/s13071-022-05619-7pubmed: 36732768google scholar: lookup
  9. Maloney JG, Molokin A, Seguí R, Maravilla P, Martínez-Hernández F, Villalobos G, Tsaousis AD, Gentekaki E, Muñoz-Antolí C, Klisiowicz DR, Oishi CY, Toledo R, Esteban JG, Köster PC, de Lucio A, Dashti A, Bailo B, Calero-Bernal R, González-Barrio D, Carmena D, Santín M. Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38.. Microorganisms 2022 Dec 23;11(1).
    doi: 10.3390/microorganisms11010046pubmed: 36677338google scholar: lookup
  10. Jiménez P, Muñoz M, Ramírez JD. An update on the distribution of Blastocystis subtypes in the Americas.. Heliyon 2022 Dec;8(12):e12592.
    doi: 10.1016/j.heliyon.2022.e12592pubmed: 36619449google scholar: lookup
  11. Naguib D, Gantois N, Desramaut J, Arafat N, Even G, Certad G, Chabé M, Viscogliosi E. Prevalence, Subtype Distribution and Zoonotic Significance of Blastocystis sp. Isolates from Poultry, Cattle and Pets in Northern Egypt.. Microorganisms 2022 Nov 14;10(11).
    doi: 10.3390/microorganisms10112259pubmed: 36422329google scholar: lookup
  12. Martín-Escolano R, Ng GC, Tan KSW, Stensvold CR, Gentekaki E, Tsaousis AD. Resistance of Blastocystis to chlorine and hydrogen peroxide.. Parasitol Res 2023 Jan;122(1):167-176.
    doi: 10.1007/s00436-022-07713-2pubmed: 36378332google scholar: lookup
  13. Zhang P, Zhang Q, Han S, Yuan G, Bai J, He H. Occurrence and Genetic Diversity of the Zoonotic Enteric Protozoans and Enterocytozoon bieneusi in Père David's Deer (Elaphurus davidianus) from Beijing, China.. Pathogens 2022 Oct 23;11(11).
    doi: 10.3390/pathogens11111223pubmed: 36364973google scholar: lookup
  14. Ahmed SA, El-Mahallawy HS, Mohamed SF, Angelici MC, Hasapis K, Saber T, Karanis P. Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt.. Sci Rep 2022 Nov 9;12(1):19084.
    doi: 10.1038/s41598-022-23360-0pubmed: 36351984google scholar: lookup
  15. Sardinha-Silva A, Alves-Ferreira EVC, Grigg ME. Intestinal immune responses to commensal and pathogenic protozoa.. Front Immunol 2022;13:963723.
    doi: 10.3389/fimmu.2022.963723pubmed: 36211380google scholar: lookup
  16. Baek S, Maloney JG, Molokin A, George NS, Cortés Vecino JA, Santin M. Diversity of Blastocystis Subtypes in Horses in Colombia and Identification of Two New Subtypes.. Microorganisms 2022 Aug 24;10(9).
    doi: 10.3390/microorganisms10091693pubmed: 36144295google scholar: lookup
  17. Shams M, Asghari A, Baniasad M, Shamsi L, Sadrebazzaz A. Blastocystis sp. in Small Ruminants: A Universal Systematic Review and Meta-analysis.. Acta Parasitol 2022 Sep;67(3):1073-1085.
    doi: 10.1007/s11686-022-00589-3pubmed: 35834160google scholar: lookup
  18. Chen S, Meng W, Shi X, Chai Y, Zhou Z, Liu H, Zhong Z, Fu H, Cao S, Ma X, Shen L, Deng L, Peng G. Occurrence, genetic diversity and zoonotic potential of Blastocystis sp. in forest musk deer (Moschus berezovskii) in Southwest China.. Parasite 2022;29:34.
    doi: 10.1051/parasite/2022037pubmed: 35833784google scholar: lookup
  19. Shams M, Shamsi L, Yousefi A, Sadrebazzaz A, Asghari A, Mohammadi-Ghalehbin B, Shahabi S, Hatam G. Current global status, subtype distribution and zoonotic significance of Blastocystis in dogs and cats: a systematic review and meta-analysis.. Parasit Vectors 2022 Jun 22;15(1):225.
    doi: 10.1186/s13071-022-05351-2pubmed: 35733146google scholar: lookup
  20. Rudzińska M, Kowalewska B, Kurpas M, Szostakowska B. Rare Occurrence of Blastocystis in Pet Animals and Their Owners in the Pomeranian Voivodeship in Poland in the Light of Literature Data.. J Clin Med 2022 May 25;11(11).
    doi: 10.3390/jcm11112975pubmed: 35683363google scholar: lookup
  21. Guilavogui T, Gantois N, Even G, Desramaut J, Dautel E, Denoyelle C, Cissé FI, Touré SC, Kourouma BL, Sawant M, Chabé M, Certad G, Viscogliosi E. Detection, Molecular Identification and Transmission of the Intestinal Protozoa Blastocystis sp. in Guinea from a Large-Scale Epidemiological Study Conducted in the Conakry Area.. Microorganisms 2022 Feb 15;10(2).
    doi: 10.3390/microorganisms10020446pubmed: 35208899google scholar: lookup
  22. Köster PC, Martínez-Nevado E, González A, Abelló-Poveda MT, Fernández-Bellon H, de la Riva-Fraga M, Marquet B, Guéry JP, Knauf-Witzens T, Weigold A, Dashti A, Bailo B, Imaña E, Muadica AS, González-Barrio D, Ponce-Gordo F, Calero-Bernal R, Carmena D. Intestinal Protists in Captive Non-human Primates and Their Handlers in Six European Zoological Gardens. Molecular Evidence of Zoonotic Transmission.. Front Vet Sci 2021;8:819887.
    doi: 10.3389/fvets.2021.819887pubmed: 35059456google scholar: lookup
  23. Liu X, Ni F, Wang R, Li J, Ge Y, Yang X, Qi M, Zhang L. Occurrence and subtyping of Blastocystis in coypus (Myocastor coypus) in China.. Parasit Vectors 2022 Jan 6;15(1):14.
    doi: 10.1186/s13071-021-05126-1pubmed: 34991712google scholar: lookup
  24. Köster PC, Lapuente J, Pizarro A, Prieto-Pérez L, Pérez-Tanoira R, Dashti A, Bailo B, Muadica AS, González-Barrio D, Calero-Bernal R, Ponce-Gordo F, Carmena D. Presence and genetic diversity of enteric protists in captive and semi-captive non-human primates in côte d'Ivoire, Sierra Leone, and Peru.. Int J Parasitol Parasites Wildl 2022 Apr;17:26-34.
    doi: 10.1016/j.ijppaw.2021.12.004pubmed: 34976722google scholar: lookup
  25. Jinatham V, Maxamhud S, Popluechai S, Tsaousis AD, Gentekaki E. Blastocystis One Health Approach in a Rural Community of Northern Thailand: Prevalence, Subtypes and Novel Transmission Routes.. Front Microbiol 2021;12:746340.
    doi: 10.3389/fmicb.2021.746340pubmed: 34956115google scholar: lookup
  26. Yang X, Li Y, Wang Y, Wang J, Lai P, Li Y, Song J, Qi M, Zhao G. Molecular Characterization of Blastocystis sp. in Camelus bactrianus in Northwestern China.. Animals (Basel) 2021 Oct 20;11(11).
    doi: 10.3390/ani11113016pubmed: 34827749google scholar: lookup
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