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Home / Equine Research Bank / J Immunol / IgE-Binding Monocytes Have an Enhanced Ability to Produce IL...
Journal of immunology (Baltimore, Md. : 1950)2021; 206(10); 2312-2321; doi: 10.4049/jimmunol.2001354

IgE-Binding Monocytes Have an Enhanced Ability to Produce IL-8 (CXCL8) in Animals with Naturally Occurring Allergy.

Abstract: IL-8 is a potent chemokine that recruits neutrophils and basophils to promote inflammation in many species. IL-8 is produced by many cell types, including monocytes. In this study, we report a novel role for IgE-binding monocytes, a rare peripheral immune cell type, to promote allergic inflammation through IL-8 production in a horse model of natural IgE-mediated allergy. We developed a mAb with confirmed specificity for both recombinant and native equine IL-8 for flow cytometric analysis. Equine IL-8 was produced by CD14/MHC class II/CD16 monocytes, including a subpopulation of IgE-binding monocytes, following stimulation with LPS. In addition, IgE cross-linking induced IL-8 production by both peripheral blood basophils and IgE-binding monocytes. IL-8 production was compared between healthy horses and those with a naturally occurring IgE-mediated skin allergy, hypersensitivity. Allergic horses had significantly higher percentages of IL-8 IgE-binding monocytes after IgE cross-linking. In contrast, frequencies of IL-8 basophils after IgE cross-linking were similar in all horses, regardless of allergic disease, highlighting IgE-binding monocytes as a novel source of IL-8 during allergy. We concluded that IgE-binding monocytes from allergic individuals have an increased capacity for IL-8 production and likely contribute to the recruitment of innate immune cells during IgE-mediated allergy and promotion of inflammation during repeated allergen contact.
Copyright © 2021 by The American Association of Immunologists, Inc.
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Publication Date: 2021-05-05 PubMed ID: 33952617PubMed Central: PMC8185406DOI: 10.4049/jimmunol.2001354Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research reveals that IgE-binding monocytes, a unique type of immune cell found in the periphery, can contribute to allergic inflammation by creating IL-8, a chemical that attracts inflammatory cells. This was discovered through experiments using a horse model designed to simulate natural IgE-mediated allergies.

Explanation of the Research

The research paper primarily focused on the role of IgE-binding monocytes, a set of peripheral immune cells, in generating allergic inflammation. The experiments were conducted using a horse model of naturally occurring IgE-mediated allergies.

Analysis Techniques

  • The researchers developed a monoclonal antibody (mAb) that could identify both native and recombinant equine IL-8, a potent chemical signal involved in inflammation. They used flow cytometric analysis to quantify this.
  • The IL-8 was predominantly produced by CD14/MHC class II/CD16 monocytes, which included a subset of IgE-binding monocytes, when prompted with Lipopolysaccharides (LPS).
  • Further, after cross-linking IgE, IL-8 production was also initiated by peripheral blood basophils and IgE-binding monocytes.

Findings

  • The researchers drew comparisons between healthy horses and those suffering from naturally occurring IgE-mediated allergies, particularly hypersensitivity.
  • They discovered that allergic horses had a significantly higher percentage of IL-8-producing IgE-binding monocytes after IgE cross-linking.
  • Interestingly, the frequencies of IL-8-producing basophils after IgE cross-linking were comparable in all horses, irrespective of their health condition. This comparison helped identify IgE-binding monocytes as a new source of IL-8 during allergic reactions.

Conclusions

  • The study concluded that IgE-binding monocytes in individuals with allergies have a heightened capacity to produce IL-8.
  • These cells are likely responsible for the recruitment of innate immune cells during IgE-mediated allergies and the promotion of inflammation during repeated allergen exposure.

Cite This Article

APA
Larson EM, Babasyan S, Wagner B. (2021). IgE-Binding Monocytes Have an Enhanced Ability to Produce IL-8 (CXCL8) in Animals with Naturally Occurring Allergy. J Immunol, 206(10), 2312-2321. https://doi.org/10.4049/jimmunol.2001354

Publication

Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
NlmUniqueID: 2985117R
Country: United States
Language: English
Volume: 206
Issue: 10
Pages: 2312-2321

Researcher Affiliations

Larson, Elisabeth M
  • Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY.
Babasyan, Susanna
  • Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY.
Wagner, Bettina
  • Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY bw73@cornell.edu.

MeSH Terms

  • Allergens / immunology
  • Animals
  • Antibodies, Monoclonal / immunology
  • Basophils / immunology
  • CHO Cells
  • Ceratopogonidae / immunology
  • Cricetulus
  • Horse Diseases / blood
  • Horse Diseases / immunology
  • Horses
  • Hybridomas
  • Hypersensitivity / blood
  • Hypersensitivity / immunology
  • Hypersensitivity / veterinary
  • Immunization / methods
  • Immunoglobulin E / metabolism
  • Interleukin-8 / administration & dosage
  • Interleukin-8 / biosynthesis
  • Interleukin-8 / genetics
  • Interleukin-8 / immunology
  • Mice
  • Mice, Inbred C57BL
  • Monocytes / immunology
  • Monocytes / metabolism
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / immunology
  • Transfection

Grant Funding

  • 2015-67015-23072 / National Institute of Food and Agriculture
  • 2019-67015-29833 / National Institute of Food and Agriculture
  • 2005-01812 / National Institute of Food and Agriculture

Conflict of Interest Statement

The authors have no financial conflicts of interest.

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Citations

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