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Journal of immunology (Baltimore, Md. : 1950)2021; 206(10); 2312-2321; doi: 10.4049/jimmunol.2001354

IgE-Binding Monocytes Have an Enhanced Ability to Produce IL-8 (CXCL8) in Animals with Naturally Occurring Allergy.

Abstract: IL-8 is a potent chemokine that recruits neutrophils and basophils to promote inflammation in many species. IL-8 is produced by many cell types, including monocytes. In this study, we report a novel role for IgE-binding monocytes, a rare peripheral immune cell type, to promote allergic inflammation through IL-8 production in a horse model of natural IgE-mediated allergy. We developed a mAb with confirmed specificity for both recombinant and native equine IL-8 for flow cytometric analysis. Equine IL-8 was produced by CD14/MHC class II/CD16 monocytes, including a subpopulation of IgE-binding monocytes, following stimulation with LPS. In addition, IgE cross-linking induced IL-8 production by both peripheral blood basophils and IgE-binding monocytes. IL-8 production was compared between healthy horses and those with a naturally occurring IgE-mediated skin allergy, hypersensitivity. Allergic horses had significantly higher percentages of IL-8 IgE-binding monocytes after IgE cross-linking. In contrast, frequencies of IL-8 basophils after IgE cross-linking were similar in all horses, regardless of allergic disease, highlighting IgE-binding monocytes as a novel source of IL-8 during allergy. We concluded that IgE-binding monocytes from allergic individuals have an increased capacity for IL-8 production and likely contribute to the recruitment of innate immune cells during IgE-mediated allergy and promotion of inflammation during repeated allergen contact.
Copyright © 2021 by The American Association of Immunologists, Inc.
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Publication Date: 2021-05-05 PubMed ID: 33952617PubMed Central: PMC8185406DOI: 10.4049/jimmunol.2001354Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research reveals that IgE-binding monocytes, a unique type of immune cell found in the periphery, can contribute to allergic inflammation by creating IL-8, a chemical that attracts inflammatory cells. This was discovered through experiments using a horse model designed to simulate natural IgE-mediated allergies.

Explanation of the Research

The research paper primarily focused on the role of IgE-binding monocytes, a set of peripheral immune cells, in generating allergic inflammation. The experiments were conducted using a horse model of naturally occurring IgE-mediated allergies.

Analysis Techniques

  • The researchers developed a monoclonal antibody (mAb) that could identify both native and recombinant equine IL-8, a potent chemical signal involved in inflammation. They used flow cytometric analysis to quantify this.
  • The IL-8 was predominantly produced by CD14/MHC class II/CD16 monocytes, which included a subset of IgE-binding monocytes, when prompted with Lipopolysaccharides (LPS).
  • Further, after cross-linking IgE, IL-8 production was also initiated by peripheral blood basophils and IgE-binding monocytes.

Findings

  • The researchers drew comparisons between healthy horses and those suffering from naturally occurring IgE-mediated allergies, particularly hypersensitivity.
  • They discovered that allergic horses had a significantly higher percentage of IL-8-producing IgE-binding monocytes after IgE cross-linking.
  • Interestingly, the frequencies of IL-8-producing basophils after IgE cross-linking were comparable in all horses, irrespective of their health condition. This comparison helped identify IgE-binding monocytes as a new source of IL-8 during allergic reactions.

Conclusions

  • The study concluded that IgE-binding monocytes in individuals with allergies have a heightened capacity to produce IL-8.
  • These cells are likely responsible for the recruitment of innate immune cells during IgE-mediated allergies and the promotion of inflammation during repeated allergen exposure.

Cite This Article

APA
Larson EM, Babasyan S, Wagner B. (2021). IgE-Binding Monocytes Have an Enhanced Ability to Produce IL-8 (CXCL8) in Animals with Naturally Occurring Allergy. J Immunol, 206(10), 2312-2321. https://doi.org/10.4049/jimmunol.2001354

Publication

Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
NlmUniqueID: 2985117R
Country: United States
Language: English
Volume: 206
Issue: 10
Pages: 2312-2321

Researcher Affiliations

Larson, Elisabeth M
  • Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY.
Babasyan, Susanna
  • Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY.
Wagner, Bettina
  • Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY bw73@cornell.edu.

MeSH Terms

  • Allergens / immunology
  • Animals
  • Antibodies, Monoclonal / immunology
  • Basophils / immunology
  • CHO Cells
  • Ceratopogonidae / immunology
  • Cricetulus
  • Horse Diseases / blood
  • Horse Diseases / immunology
  • Horses
  • Hybridomas
  • Hypersensitivity / blood
  • Hypersensitivity / immunology
  • Hypersensitivity / veterinary
  • Immunization / methods
  • Immunoglobulin E / metabolism
  • Interleukin-8 / administration & dosage
  • Interleukin-8 / biosynthesis
  • Interleukin-8 / genetics
  • Interleukin-8 / immunology
  • Mice
  • Mice, Inbred C57BL
  • Monocytes / immunology
  • Monocytes / metabolism
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / immunology
  • Transfection

Grant Funding

  • 2015-67015-23072 / National Institute of Food and Agriculture
  • 2019-67015-29833 / National Institute of Food and Agriculture
  • 2005-01812 / National Institute of Food and Agriculture

Conflict of Interest Statement

The authors have no financial conflicts of interest.

References

This article includes 82 references
  1. Larsen CG, Anderson AO, Appella E, Oppenheim JJ, Matsushima K. The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes.. Science 1989 Mar 17;243(4897):1464-6.
    pubmed: 2648569doi: 10.1126/science.2648569google scholar: lookup
  2. Van Damme J, Van Beeumen J, Conings R, Decock B, Billiau A. Purification of granulocyte chemotactic peptide/interleukin-8 reveals N-terminal sequence heterogeneity similar to that of beta-thromboglobulin.. Eur J Biochem 1989 May 1;181(2):337-44.
    pubmed: 2523801doi: 10.1111/j.1432-1033.1989.tb14729.xgoogle scholar: lookup
  3. Baggiolini M, Clark-Lewis I. Interleukin-8, a chemotactic and inflammatory cytokine.. FEBS Lett 1992 Jul 27;307(1):97-101.
    pubmed: 1639201doi: 10.1016/0014-5793(92)80909-zgoogle scholar: lookup
  4. Krieger M, Brunner T, Bischoff SC, von Tscharner V, Walz A, Moser B, Baggiolini M, Dahinden CA. Activation of human basophils through the IL-8 receptor.. J Immunol 1992 Oct 15;149(8):2662-7.
    pubmed: 1383321
  5. Youssef LA, Schuyler M, Gilmartin L, Pickett G, Bard JD, Tarleton CA, Archibeque T, Qualls C, Wilson BS, Oliver JM. Histamine release from the basophils of control and asthmatic subjects and a comparison of gene expression between "releaser" and "nonreleaser" basophils.. J Immunol 2007 Apr 1;178(7):4584-94.
    pubmed: 17372017doi: 10.4049/jimmunol.178.7.4584google scholar: lookup
  6. Youssef LA, Schuyler M, Wilson BS, Oliver JM. Roles for the High Affinity IgE Receptor, FcεRI, of Human Basophils in the Pathogenesis and Therapy of Allergic Asthma: Disease Promotion, Protection or Both?. Open Allergy J 2010;3:91-101.
    pmc: PMC4090948pubmed: 25018787doi: 10.2174/1874838401003010091google scholar: lookup
  7. Gilmartin L, Tarleton CA, Schuyler M, Wilson BS, Oliver JM. A comparison of inflammatory mediators released by basophils of asthmatic and control subjects in response to high-affinity IgE receptor aggregation.. Int Arch Allergy Immunol 2008;145(3):182-92.
    pubmed: 17912006doi: 10.1159/000109287google scholar: lookup
  8. Baggiolini M, Walz A, Kunkel SL. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils.. J Clin Invest 1989 Oct;84(4):1045-9.
    pmc: PMC329758pubmed: 2677047doi: 10.1172/jci114265google scholar: lookup
  9. Leclere M, Lavoie-Lamoureux A, Lavoie JP. Heaves, an asthma-like disease of horses.. Respirology 2011 Oct;16(7):1027-46.
    pubmed: 21824219doi: 10.1111/j.1440-1843.2011.02033.xgoogle scholar: lookup
  10. Nilsson G, Mikovits JA, Metcalfe DD, Taub DD. Mast cell migratory response to interleukin-8 is mediated through interaction with chemokine receptor CXCR2/Interleukin-8RB.. Blood 1999 May 1;93(9):2791-7.
    pubmed: 10216072
  11. Ha H, Debnath B, Neamati N. Role of the CXCL8-CXCR1/2 Axis in Cancer and Inflammatory Diseases.. Theranostics 2017;7(6):1543-1588.
    pmc: PMC5436513pubmed: 28529637doi: 10.7150/thno.15625google scholar: lookup
  12. Lippert U, Artuc M, Grützkau A, Möller A, Kenderessy-Szabo A, Schadendorf D, Norgauer J, Hartmann K, Schweitzer-Stenner R, Zuberbier T, Henz BM, Krüger-Krasagakes S. Expression and functional activity of the IL-8 receptor type CXCR1 and CXCR2 on human mast cells.. J Immunol 1998 Sep 1;161(5):2600-8.
    pubmed: 9725262
  13. Möller A, Lippert U, Lessmann D, Kolde G, Hamann K, Welker P, Schadendorf D, Rosenbach T, Luger T, Czarnetzki BM. Human mast cells produce IL-8.. J Immunol 1993 Sep 15;151(6):3261-6.
    pubmed: 8376778
  14. Kulka M, Sheen CH, Tancowny BP, Grammer LC, Schleimer RP. Neuropeptides activate human mast cell degranulation and chemokine production.. Immunology 2008 Mar;123(3):398-410.
    pmc: PMC2433325pubmed: 17922833doi: 10.1111/j.1365-2567.2007.02705.xgoogle scholar: lookup
  15. Feuser K, Feilhauer K, Staib L, Bischoff SC, Lorentz A. Akt cross-links IL-4 priming, stem cell factor signaling, and IgE-dependent activation in mature human mast cells.. Mol Immunol 2011 Jan;48(4):546-52.
    pubmed: 21106245doi: 10.1016/j.molimm.2010.10.010google scholar: lookup
  16. Steiner M, Hawranek T, Schneider M, Ferreira F, Horejs-Hoeck J, Harrer A, Himly M. Elevated Toll-Like Receptor-Induced CXCL8 Secretion in Human Blood Basophils from Allergic Donors Is Independent of Toll-Like Receptor Expression Levels.. PLoS One 2016;11(2):e0149275.
    pmc: PMC4752351pubmed: 26870962doi: 10.1371/journal.pone.0149275google scholar: lookup
  17. Oliver JM, Tarleton CA, Gilmartin L, Archibeque T, Qualls CR, Diehl L, Wilson BS, Schuyler M. Reduced FcepsilonRI-mediated release of asthma-promoting cytokines and chemokines from human basophils during omalizumab therapy.. Int Arch Allergy Immunol 2010;151(4):275-84.
    pmc: PMC2853585pubmed: 19844128doi: 10.1159/000250436google scholar: lookup
  18. Pecaric-Petkovic T, Didichenko SA, Kaempfer S, Spiegl N, Dahinden CA. Human basophils and eosinophils are the direct target leukocytes of the novel IL-1 family member IL-33.. Blood 2009 Feb 12;113(7):1526-34.
    pmc: PMC2644080pubmed: 18955562doi: 10.1182/blood-2008-05-157818google scholar: lookup
  19. Novak N, Bieber T, Katoh N. Engagement of Fc epsilon RI on human monocytes induces the production of IL-10 and prevents their differentiation in dendritic cells.. J Immunol 2001 Jul 15;167(2):797-804.
    pubmed: 11441085doi: 10.4049/jimmunol.167.2.797google scholar: lookup
  20. Pyle DM, Yang VS, Gruchalla RS, Farrar JD, Gill MA. IgE cross-linking critically impairs human monocyte function by blocking phagocytosis.. J Allergy Clin Immunol 2013 Feb;131(2):491-500.e1-5.
    pmc: PMC3564054pubmed: 23374271doi: 10.1016/j.jaci.2012.11.037google scholar: lookup
  21. Lee J, Cacalano G, Camerato T, Toy K, Moore MW, Wood WI. Chemokine binding and activities mediated by the mouse IL-8 receptor.. J Immunol 1995 Aug 15;155(4):2158-64.
    pubmed: 7636264
  22. Hol J, Wilhelmsen L, Haraldsen G. The murine IL-8 homologues KC, MIP-2, and LIX are found in endothelial cytoplasmic granules but not in Weibel-Palade bodies.. J Leukoc Biol 2010 Mar;87(3):501-8.
    pubmed: 20007247doi: 10.1189/jlb.0809532google scholar: lookup
  23. Boisvert WA, Santiago R, Curtiss LK, Terkeltaub RA. A leukocyte homologue of the IL-8 receptor CXCR-2 mediates the accumulation of macrophages in atherosclerotic lesions of LDL receptor-deficient mice.. J Clin Invest 1998 Jan 15;101(2):353-63.
    pmc: PMC508574pubmed: 9435307doi: 10.1172/jci1195google scholar: lookup
  24. Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW. Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8.. J Biol Chem 2007 Apr 20;282(16):11658-66.
    pubmed: 17197447doi: 10.1074/jbc.m607705200google scholar: lookup
  25. Horohov DW. The equine immune responses to infectious and allergic disease: a model for humans?. Mol Immunol 2015 Jul;66(1):89-96.
    pubmed: 25457878doi: 10.1016/j.molimm.2014.09.020google scholar: lookup
  26. Derksen FJ, Scott JS, Miller DC, Slocombe RF, Robinson NE. Bronchoalveolar lavage in ponies with recurrent airway obstruction (heaves).. Am Rev Respir Dis 1985 Nov;132(5):1066-70.
    pubmed: 4062037doi: 10.1164/arrd.1985.132.5.1066google scholar: lookup
  27. Ainsworth DM, Grünig G, Matychak MB, Young J, Wagner B, Erb HN, Antczak DF. Recurrent airway obstruction (RAO) in horses is characterized by IFN-gamma and IL-8 production in bronchoalveolar lavage cells.. Vet Immunol Immunopathol 2003 Nov 15;96(1-2):83-91.
    pubmed: 14522137doi: 10.1016/s0165-2427(03)00142-9google scholar: lookup
  28. Ainsworth DM, Wagner B, Franchini M, Grünig G, Erb HN, Tan JY. Time-dependent alterations in gene expression of interleukin-8 in the bronchial epithelium of horses with recurrent airway obstruction.. Am J Vet Res 2006 Apr;67(4):669-77.
    pubmed: 16579761doi: 10.2460/ajvr.67.4.669google scholar: lookup
  29. Ainsworth DM, Wagner B, Erb HN, Young JC, Retallick DE. Effects of in vitro exposure to hay dust on expression of interleukin-17, -23, -8, and -1beta and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells isolated from horses chronically affected with recurrent airway disease.. Am J Vet Res 2007 Dec;68(12):1361-9.
    pubmed: 18052742doi: 10.2460/ajvr.68.12.1361google scholar: lookup
  30. Giguère S, Viel L, Lee E, MacKay RJ, Hernandez J, Franchini M. Cytokine induction in pulmonary airways of horses with heaves and effect of therapy with inhaled fluticasone propionate.. Vet Immunol Immunopathol 2002 Mar;85(3-4):147-58.
    pubmed: 11943316doi: 10.1016/s0165-2427(01)00420-2google scholar: lookup
  31. Franchini M, Gill U, von Fellenberg R, Bracher VD. Interleukin-8 concentration and neutrophil chemotactic activity in bronchoalveolar lavage fluid of horses with chronic obstructive pulmonary disease following exposure to hay.. Am J Vet Res 2000 Nov;61(11):1369-74.
    pubmed: 11108181doi: 10.2460/ajvr.2000.61.1369google scholar: lookup
  32. Reyner CL, Wagner B, Young JC, Ainsworth DM. Effects of in vitro exposure to hay dust on expression of interleukin-23, -17, -8, and -1beta and chemokine (C-X-C motif) ligand 2 by pulmonary mononuclear cells from horses susceptible to recurrent airway obstruction.. Am J Vet Res 2009 Oct;70(10):1277-83.
    pubmed: 19795943doi: 10.2460/ajvr.70.10.1277google scholar: lookup
  33. Hughes KJ, Nicolson L, Da Costa N, Franklin SH, Allen KJ, Dunham SP. Evaluation of cytokine mRNA expression in bronchoalveolar lavage cells from horses with inflammatory airway disease.. Vet Immunol Immunopathol 2011 Mar 15;140(1-2):82-9.
    pubmed: 21194756doi: 10.1016/j.vetimm.2010.11.018google scholar: lookup
  34. Anderson GS, Belton P, Kleider N. Hypersensitivity of horses in British Columbia to extracts of native and exotic species of Culicoides (Diptera: Ceratopogonidae).. J Med Entomol 1993 Jul;30(4):657-63.
    pubmed: 8360890doi: 10.1093/jmedent/30.4.657google scholar: lookup
  35. Björnsdóttir S, Sigvaldadóttir J, Broström H, Langvad B, Sigurdsson A. Summer eczema in exported Icelandic horses: influence of environmental and genetic factors.. Acta Vet Scand 2006 May 26;48(1):3.
    pmc: PMC1513129pubmed: 16987399doi: 10.1186/1751-0147-48-3google scholar: lookup
  36. Braverman Y. Preferred landing sites of Culicoides species (Diptera: Ceratopogonidae) on a horse in Israel and its relevance to summer seasonal recurrent dermatitis (sweet itch).. Equine Vet J 1988 Nov;20(6):426-9.
    pubmed: 3215168doi: 10.1111/j.2042-3306.1988.tb01566.xgoogle scholar: lookup
  37. Greiner EC, Fadok VA, Rabin EB. Equine Culicoides hypersensitivity in Florida: biting midges aspirated from horses.. Med Vet Entomol 1990 Oct;4(4):375-81.
    pubmed: 2133005doi: 10.1111/j.1365-2915.1990.tb00454.xgoogle scholar: lookup
  38. Littlewood JD. Incidence of recurrent seasonal pruritus ('sweet itch') in British and German shire horses.. Vet Rec 1998 Jan 17;142(3):66-7.
    pubmed: 9481844doi: 10.1136/vr.142.3.66google scholar: lookup
  39. Steinman A, Peer G, Klement E. Epidemiological study of Culicoides hypersensitivity in horses in Israel.. Vet Rec 2003 Jun 14;152(24):748-51.
    pubmed: 12833935doi: 10.1136/vr.152.24.748google scholar: lookup
  40. Pilsworth RC, Knottenbelt DC. Skin diseases refresher equine insect hypersensitivity disease profile. Equine Vet. Educ. 2004;16:324–325.
  41. Wagner B. The Immune System of Horses and Other Equids. Encyclopedia of Immunobiology 2016; p. 549–555.
  42. Larsen HJ, Bakke SH, Mehl R. Intradermal challenge of Icelandic horses in Norway and Iceland with extracts of Culicoides spp.. Acta Vet Scand 1988;29(3-4):311-4.
    pmc: PMC8161629pubmed: 3256230doi: 10.1186/bf03548623google scholar: lookup
  43. Schaffartzik A, Marti E, Crameri R, Rhyner C. Cloning, production and characterization of antigen 5 like proteins from Simulium vittatum and Culicoides nubeculosus, the first cross-reactive allergen associated with equine insect bite hypersensitivity.. Vet Immunol Immunopathol 2010 Sep 15;137(1-2):76-83.
    pubmed: 20537727doi: 10.1016/j.vetimm.2010.04.012google scholar: lookup
  44. Schaffartzik A, Marti E, Torsteinsdottir S, Mellor PS, Crameri R, Rhyner C. Selective cloning, characterization, and production of the Culicoides nubeculosus salivary gland allergen repertoire associated with equine insect bite hypersensitivity.. Vet Immunol Immunopathol 2011 Feb 15;139(2-4):200-9.
    pubmed: 21071100doi: 10.1016/j.vetimm.2010.10.015google scholar: lookup
  45. Schaffartzik A, Hamza E, Janda J, Crameri R, Marti E, Rhyner C. Equine insect bite hypersensitivity: what do we know?. Vet Immunol Immunopathol 2012 Jun 30;147(3-4):113-26.
    pubmed: 22575371doi: 10.1016/j.vetimm.2012.03.017google scholar: lookup
  46. van der Meide NM, Roders N, Sloet van Oldruitenborgh-Oosterbaan MM, Schaap PJ, van Oers MM, Leibold W, Savelkoul HF, Tijhaar E. Cloning and expression of candidate allergens from Culicoides obsoletus for diagnosis of insect bite hypersensitivity in horses.. Vet Immunol Immunopathol 2013 Jun 15;153(3-4):227-39.
    pubmed: 23561552doi: 10.1016/j.vetimm.2013.03.005google scholar: lookup
  47. Wagner B, Miller WH Jr, Erb HN, Lunn DP, Antczak DF. Sensitization of skin mast cells with IgE antibodies to Culicoides allergens occurs frequently in clinically healthy horses.. Vet Immunol Immunopathol 2009 Nov 15;132(1):53-61.
    pubmed: 19836083doi: 10.1016/j.vetimm.2009.09.015google scholar: lookup
  48. Wagner B, Stokol T, Ainsworth DM. Induction of interleukin-4 production in neonatal IgE+ cells after crosslinking of maternal IgE.. Dev Comp Immunol 2010 Apr;34(4):436-44.
    pubmed: 19995577doi: 10.1016/j.dci.2009.12.002google scholar: lookup
  49. Wagner B, Miller WH, Morgan EE, Hillegas JM, Erb HN, Leibold W, Antczak DF. IgE and IgG antibodies in skin allergy of the horse.. Vet Res 2006 Nov-Dec;37(6):813-25.
    pubmed: 16973120doi: 10.1051/vetres:2006039google scholar: lookup
  50. Wagner B, Childs BA, Erb HN. A histamine release assay to identify sensitization to Culicoides allergens in horses with skin hypersensitivity.. Vet Immunol Immunopathol 2008 Dec 15;126(3-4):302-8.
    pubmed: 18926574doi: 10.1016/j.vetimm.2008.09.001google scholar: lookup
  51. Larson EM, Babasyan S, Wagner B. Phenotype and function of IgE-binding monocytes in equine Culicoides hypersensitivity.. PLoS One 2020;15(5):e0233537.
    pmc: PMC7244122pubmed: 32442209doi: 10.1371/journal.pone.0233537google scholar: lookup
  52. Deleuran M, Vestergaard C. Clinical heterogeneity and differential diagnosis of atopic dermatitis.. Br J Dermatol 2014 Jul;170 Suppl 1:2-6.
    pubmed: 24720512doi: 10.1111/bjd.12933google scholar: lookup
  53. Álvarez-Errico D, Oliver-Vila I, Ainsua-Enrich E, Gilfillan AM, Picado C, Sayós J, Martín M. CD84 negatively regulates IgE high-affinity receptor signaling in human mast cells.. J Immunol 2011 Dec 1;187(11):5577-86.
    pmc: PMC3233232pubmed: 22068234doi: 10.4049/jimmunol.1101626google scholar: lookup
  54. Wagner B, Hillegas JM, Babasyan S. Monoclonal antibodies to equine CD23 identify the low-affinity receptor for IgE on subpopulations of IgM+ and IgG1+ B-cells in horses.. Vet Immunol Immunopathol 2012 Apr 15;146(2):125-34.
    pubmed: 22405681doi: 10.1016/j.vetimm.2012.02.007google scholar: lookup
  55. Schnabel CL, Wemette M, Babasyan S, Freer H, Baldwin C, Wagner B. C-C motif chemokine ligand (CCL) production in equine peripheral blood mononuclear cells identified by newly generated monoclonal antibodies.. Vet Immunol Immunopathol 2018 Oct;204:28-39.
    pubmed: 30596378doi: 10.1016/j.vetimm.2018.09.003google scholar: lookup
  56. Wagner B, Hillegas JM, Antczak DF. A monoclonal antibody to equine interleukin-4.. Vet Immunol Immunopathol 2006 Apr 15;110(3-4):363-7.
    pubmed: 16480777doi: 10.1016/j.vetimm.2006.01.001google scholar: lookup
  57. Wagner B, Radbruch A, Rohwer J, Leibold W. Monoclonal anti-equine IgE antibodies with specificity for different epitopes on the immunoglobulin heavy chain of native IgE.. Vet Immunol Immunopathol 2003 Mar 20;92(1-2):45-60.
    pubmed: 12628763doi: 10.1016/s0165-2427(03)00007-2google scholar: lookup
  58. Schnabel CL, Babasyan S, Freer H, Wagner B. CXCL10 production in equine monocytes is stimulated by interferon-gamma.. Vet Immunol Immunopathol 2019 Jan;207:25-30.
    pubmed: 30593347doi: 10.1016/j.vetimm.2018.11.016google scholar: lookup
  59. Kearney JF, Radbruch A, Liesegang B, Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.. J Immunol 1979 Oct;123(4):1548-50.
    pubmed: 113458
  60. Miller JE, Mann S, Fettelschoss-Gabriel A, Wagner B. Comparison of three clinical scoring systems for Culicoides hypersensitivity in a herd of Icelandic horses.. Vet Dermatol 2019 Dec;30(6):536-e163.
    pubmed: 31441172doi: 10.1111/vde.12784google scholar: lookup
  61. Sheoran AS, Lunn DP, Holmes MA. Monoclonal antibodies to subclass-specific antigenic determinants on equine immunoglobulin gamma chains and their characterization.. Vet Immunol Immunopathol 1998 Mar 31;62(2):153-65.
    pubmed: 9638859doi: 10.1016/s0165-2427(97)00162-1google scholar: lookup
  62. Mukaida N, Matsushima K. Interleukin 8 and its Receptor. Encyclopedia of Immunology, 2nd Ed. 1998; p. 1466–1471.
  63. Dohmann K, Wagner B, Horohov DW, Leibold W. Expression and characterisation of equine interleukin 2 and interleukin 4.. Vet Immunol Immunopathol 2000 Dec 29;77(3-4):243-56.
    pubmed: 11137123doi: 10.1016/s0165-2427(00)00249-xgoogle scholar: lookup
  64. Russo RC, Garcia CC, Teixeira MM, Amaral FA. The CXCL8/IL-8 chemokine family and its receptors in inflammatory diseases.. Expert Rev Clin Immunol 2014 May;10(5):593-619.
    pubmed: 24678812doi: 10.1586/1744666x.2014.894886google scholar: lookup
  65. Joubert P, Silversides DW, Lavoie JP. Equine neutrophils express mRNA for tumour necrosis factor-alpha, interleukin (IL)-1beta, IL-6, IL-8, macrophage-inflammatory-protein-2 but not for IL-4, IL-5 and interferon-gamma.. Equine Vet J 2001 Nov;33(7):730-3.
    pubmed: 11770998doi: 10.2746/042516401776249246google scholar: lookup
  66. Stefanovic N, Flohr C, Irvine AD. The exposome in atopic dermatitis.. Allergy 2020 Jan;75(1):63-74.
    pmc: PMC7003958pubmed: 31194890doi: 10.1111/all.13946google scholar: lookup
  67. Liu T, Nerren J, Liu M, Martens R, Cohen N. Basal and stimulus-induced cytokine expression is selectively impaired in peripheral blood mononuclear cells of newborn foals.. Vaccine 2009 Jan 29;27(5):674-83.
    pubmed: 19056444doi: 10.1016/j.vaccine.2008.11.040google scholar: lookup
  68. Gould HJ, Sutton BJ. IgE in allergy and asthma today.. Nat Rev Immunol 2008 Mar;8(3):205-17.
    pubmed: 18301424doi: 10.1038/nri2273google scholar: lookup
  69. Gilfillan AM, Rivera J. The tyrosine kinase network regulating mast cell activation.. Immunol Rev 2009 Mar;228(1):149-69.
    pmc: PMC2669301pubmed: 19290926doi: 10.1111/j.1600-065x.2008.00742.xgoogle scholar: lookup
  70. Lin S, Cicala C, Scharenberg AM, Kinet JP. The Fc(epsilon)RIbeta subunit functions as an amplifier of Fc(epsilon)RIgamma-mediated cell activation signals.. Cell 1996 Jun 28;85(7):985-95.
    pubmed: 8674126doi: 10.1016/s0092-8674(00)81300-8google scholar: lookup
  71. Cruse G, Kaur D, Leyland M, Bradding P. A novel FcεRIβ-chain truncation regulates human mast cell proliferation and survival.. FASEB J 2010 Oct;24(10):4047-57.
    pmc: PMC2996906pubmed: 20554927doi: 10.1096/fj.10-158378google scholar: lookup
  72. Noronha LE, Harman RM, Wagner B, Antczak DF. Generation and characterization of monoclonal antibodies to equine CD16.. Vet Immunol Immunopathol 2012 Apr 15;146(2):135-42.
    pmc: PMC3684017pubmed: 22424928doi: 10.1016/j.vetimm.2012.02.006google scholar: lookup
  73. Kabithe E, Hillegas J, Stokol T, Moore J, Wagner B. Monoclonal antibodies to equine CD14.. Vet Immunol Immunopathol 2010 Nov 15;138(1-2):149-53.
    pubmed: 20674042doi: 10.1016/j.vetimm.2010.07.003google scholar: lookup
  74. Ziegler-Heitbrock L. Reprint of: Monocyte subsets in man and other species.. Cell Immunol 2014 Sep-Oct;291(1-2):11-5.
    pubmed: 25015741doi: 10.1016/j.cellimm.2014.06.008google scholar: lookup
  75. Ziegler-Heitbrock L. Blood Monocytes and Their Subsets: Established Features and Open Questions.. Front Immunol 2015;6:423.
    pmc: PMC4538304pubmed: 26347746doi: 10.3389/fimmu.2015.00423google scholar: lookup
  76. Patel AA, Zhang Y, Fullerton JN, Boelen L, Rongvaux A, Maini AA, Bigley V, Flavell RA, Gilroy DW, Asquith B, Macallan D, Yona S. The fate and lifespan of human monocyte subsets in steady state and systemic inflammation.. J Exp Med 2017 Jul 3;214(7):1913-1923.
    pmc: PMC5502436pubmed: 28606987doi: 10.1084/jem.20170355google scholar: lookup
  77. Guilliams M, Mildner A, Yona S. Developmental and Functional Heterogeneity of Monocytes.. Immunity 2018 Oct 16;49(4):595-613.
    pubmed: 30332628doi: 10.1016/j.immuni.2018.10.005google scholar: lookup
  78. Stansfield BK, Ingram DA. Clinical significance of monocyte heterogeneity.. Clin Transl Med 2015;4:5.
    pmc: PMC4384980pubmed: 25852821doi: 10.1186/s40169-014-0040-3google scholar: lookup
  79. Maurer D, Fiebiger E, Reininger B, Wolff-Winiski B, Jouvin MH, Kilgus O, Kinet JP, Stingl G. Expression of functional high affinity immunoglobulin E receptors (Fc epsilon RI) on monocytes of atopic individuals.. J Exp Med 1994 Feb 1;179(2):745-50.
    pmc: PMC2191351pubmed: 8294882doi: 10.1084/jem.179.2.745google scholar: lookup
  80. Shin JS, Greer AM. The role of FcεRI expressed in dendritic cells and monocytes.. Cell Mol Life Sci 2015 Jun;72(12):2349-60.
    pmc: PMC4479177pubmed: 25715742doi: 10.1007/s00018-015-1870-xgoogle scholar: lookup
  81. Cheng YX, Foster B, Holland SM, Klion AD, Nutman TB, Casale TB, Metcalfe DD, Prussin C. CD2 identifies a monocyte subpopulation with immunoglobulin E-dependent, high-level expression of Fc epsilon RI.. Clin Exp Allergy 2006 Nov;36(11):1436-45.
    pmc: PMC1661841pubmed: 17083354doi: 10.1111/j.1365-2222.2006.02578.xgoogle scholar: lookup
  82. Rüfenacht S, Marti E, von Tscharner C, Doherr MG, Forster U, Welle M, Roosje PJ. Immunoglobulin E-bearing cells and mast cells in skin biopsies of horses with urticaria.. Vet Dermatol 2005 Apr;16(2):94-101.
    pubmed: 15842539doi: 10.1111/j.1365-3164.2005.00440.xgoogle scholar: lookup

Citations

This article has been cited 7 times.
  1. Simonin EM, Wagner B. IgE-binding monocytes upregulate the coagulation cascade in allergic horses. Genes Immun 2023 Jun;24(3):130-138.
    doi: 10.1038/s41435-023-00207-wpubmed: 37193769google scholar: lookup
  2. Bao Y, Zhu X. Role of Chemokines and Inflammatory Cells in Respiratory Allergy. J Asthma Allergy 2022;15:1805-1822.
    doi: 10.2147/JAA.S395490pubmed: 36575714google scholar: lookup
  3. Cortes LM, Brodsky D, Chen C, Pridgen T, Odle J, Snider DB, Cruse G, Putikova A, Masuda MY, Doyle AD, Wright BL, Dawson HD, Blikslager A, Dellon ES, Laster SM, Käser T. Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis. Front Allergy 2022;3:1029184.
    doi: 10.3389/falgy.2022.1029184pubmed: 36452260google scholar: lookup
  4. Benedé S, Pérez-Rodríguez L, Molina E. Caco-2 Cell Response Induced by Peptides Released after Digestion of Heat-Treated Egg White Proteins. Foods 2022 Nov 9;11(22).
    doi: 10.3390/foods11223566pubmed: 36429158google scholar: lookup
  5. Gressler AE, Lübke S, Wagner B, Arnold C, Lohmann KL, Schnabel CL. Comprehensive Flow Cytometric Characterization of Bronchoalveolar Lavage Cells Indicates Comparable Phenotypes Between Asthmatic and Healthy Horses But Functional Lymphocyte Differences. Front Immunol 2022;13:896255.
    doi: 10.3389/fimmu.2022.896255pubmed: 35874777google scholar: lookup
  6. Raza A, Naseem MN, Kamran M, Nouwens A, Juli MSB, Constantinoiu C, Tabor A, Schulz B, James P. Serum proteomic profiling reveals immune and inflammatory mechanisms driving susceptibility to buffalo fly-induced skin lesions in cattle. Sci Rep 2025 Dec 3;16(1):769.
    doi: 10.1038/s41598-025-30396-5pubmed: 41339694google scholar: lookup
  7. Holmes CM, Babasyan S, Wagner B. Neonatal and maternal upregulation of antileukoproteinase in horses. Front Immunol 2024;15:1395030.
    doi: 10.3389/fimmu.2024.1395030pubmed: 38736885google scholar: lookup
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