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Respiratory research2005; 6(1); 104; doi: 10.1186/1465-9921-6-104

Immune sensitization of equine bronchus: glutathione, IL-1beta expression and tissue responsiveness.

Abstract: Increasing clinical epidemiological and experimental evidence indicates that excess of production of reactive oxygen free radicals (ROS) induced by an oxidative stress is involved in the pathogenesis of a number of human airway disorders, as well as equine recurrent airway obstruction. Free-radicals modulate the activation of transcription factors, such as nuclear factor-(NF)-kappaB and activator protein (AP)-1, in several different cells. This activation leads to expression of many pro-inflammatory cytokines, including interleukin (IL)-1beta. We have hypothesized that equine airway sensitization might induce an oxidative stress and increase the ROS production, which in turn might enhance a production of IL-1beta and airway hyperresponsiveness. Methods: We have examined the effect of passive sensitization on IL-1beta mRNA expression and electrical field stimulation (EFS)-induced contraction in equine isolated bronchi, and the potential interference of reduced-glutathione (GSH), an antioxidant, with these responses. Bronchi passively sensitized with serum from animals suffering from heaves and having high total level of IgE, and control tissues, either pretreated or not with GSH (100 microM), were used to quantify IL-1beta mRNA. Other tissues were used to study the effect of EFS (3-10-25 Hz). Results: Mean IL-1beta mRNA expression was higher in passively sensitized than in control rings. GSH significantly (p < 0.05) reduced the IL-1beta mRNA expression only in passively sensitized bronchi. ELF induced a frequency-dependent contraction in both non-sensitized and passively sensitized tissues, with a significantly greater response always observed in sensitized tissues. GSH did not modify the EFS-induced contraction in non-sensitized bronchi, but significantly (p < 0.05) decreased it in passively sensitized tissues. Conclusions: Our data indicate that the passive sensitization of equine bronchi induces inflammation and hyperresponsiveness. These effects might be due to an oxidative stress because a pretreatment with GSH decreased the increased IL-1beta mRNA expression and responsiveness to EFS of passively sensitized bronchi.
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Publication Date: 2005-09-15 PubMed ID: 16164745PubMed Central: PMC1261534DOI: 10.1186/1465-9921-6-104Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study aims to examine the impact of the oxidative stress on the development of equine recurrent airway obstruction. The study hypothesizes that excess production of reactive oxygen species due to oxidative stress may promote inflammation in the bronchial tissues of the horses, leading to hyperresponsiveness of the airway.

Research Methods

  • The researchers conducted the study in equine bronchi to explore the effect of passive sensitization (exposure to an allergen) on the expression of Interleukin (IL)-1 beta, a pro-inflammatory cytokine, and electrical field stimulation-induced contraction (a measure of bronchial responsiveness).
  • The study also examined the potential role of reduced-glutathione (GSH), an antioxidant, in moderating these responses. Bronchial tissues passively sensitized with serum from animals suffering from heaves (an equine respiratory disease) were used to quantify IL-1 beta levels.
  • The samples were treated with different frequencies of electrical field stimulation to measure the contractions.

Results

  • The study found that the expression of IL-1 beta in passively sensitized bronchial tissues was higher than in non-sensitized tissues, confirming a role in inflammation and possibly in hyperresponsiveness of the airway.
  • The antioxidant GSH was found to significantly reduce IL-1 beta expression in passively sensitized bronchial tissues, pointing to the possible role of oxidative stress in disease pathogenesis.
  • The electrical field stimulation induced a frequency-dependent contraction in both sensitized and non-sensitized tissues. However, the response was more prominent in sensitized tissues, indicating hyperresponsiveness of the bronchial tissues.
  • GSH treatment significantly reduced this contraction response in passively sensitized tissues, underscoring its potential protective role against bronchial hyperresponsiveness.

Conclusions

  • Based on these results, the study concludes that passive sensitization results in inflammation and hyperresponsiveness in equine bronchi, possibly due to oxidative stress.
  • The observed protective effect of the antioxidant GSH in reducing hyperresponsiveness and pro-inflammatory cytokine expression further reinforces this conclusion.

Cite This Article

APA
Matera MG, Calzetta L, Peli A, Scagliarini A, Matera C, Cazzola M. (2005). Immune sensitization of equine bronchus: glutathione, IL-1beta expression and tissue responsiveness. Respir Res, 6(1), 104. https://doi.org/10.1186/1465-9921-6-104

Publication

Respiratory research
ISSN: 1465-993X
NlmUniqueID: 101090633
Country: England
Language: English
Volume: 6
Issue: 1
Pages: 104

Researcher Affiliations

Matera, M G
  • Department of Experimental Medicine, 2nd University of Naples, Naples, Italy. mariagabriella.matera@unina2.it
Calzetta, L
    Peli, A
      Scagliarini, A
        Matera, C
          Cazzola, M

            MeSH Terms

            • Animals
            • Bronchi / immunology
            • Bronchi / pathology
            • Gene Expression Regulation / immunology
            • Glutathione / immunology
            • Horse Diseases / immunology
            • Horse Diseases / pathology
            • Horses
            • Immunization / methods
            • Immunization / veterinary
            • In Vitro Techniques
            • Interleukin-1 / immunology
            • Male
            • Reactive Oxygen Species / immunology
            • Respiratory Hypersensitivity / immunology
            • Respiratory Hypersensitivity / pathology
            • Respiratory Hypersensitivity / veterinary

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            Citations

            This article has been cited 3 times.
            1. Calzetta L, Matera MG, Facciolo F, Cazzola M, Rogliani P. Beclomethasone dipropionate and formoterol fumarate synergistically interact in hyperresponsive medium bronchi and small airways. Respir Res 2018 Apr 12;19(1):65.
              doi: 10.1186/s12931-018-0770-7pubmed: 29650006google scholar: lookup
            2. Cazzola M, Calzetta L, Facciolo F, Rogliani P, Matera MG. Pharmacological investigation on the anti-oxidant and anti-inflammatory activity of N-acetylcysteine in an ex vivo model of COPD exacerbation. Respir Res 2017 Jan 24;18(1):26.
              doi: 10.1186/s12931-016-0500-ypubmed: 28118826google scholar: lookup
            3. Inoue K, Koike E, Yanagisawa R, Takano H. Extensive Analysis of Elastase-Induced Pulmonary Emphysema in Rats: ALP in the Lung, a New Biomarker for Disease Progression?. J Clin Biochem Nutr 2010 Mar;46(2):168-76.
              doi: 10.3164/jcbn.09-87pubmed: 20216950google scholar: lookup
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