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In vitro and in vivo binding of phenylbutazone and related drugs to equine feeds and digesta.
Abstract: In vitro and in vivo studies of phenylbutazone binding to equine ingesta and digesta were undertaken. In vitro binding to chopped hay and powdered pony nuts in buffer solutions at 37 degrees C was found to be time-, concentration- and pH-dependent. Percentage binding generally increased with time, decreased with concentration and varied with buffer pH in an unpredictable manner. Other non-steroidal anti-inflammatory drugs (NSAIDs) also bound to hay, the degree of binding being less for meclofenamate and least for flunixin in comparison with phenylbutazone. Phenylbutazone became bound to digesta collected from eight regions of the gastrointestinal tract when they were spiked with a concentration of 1 mg.10 g-1 digesta, the amounts ranging from 80.0 per cent (duodenum) to 99.6 per cent (stomach). Binding also occurred to equine digesta following the oral administration of phenylbutazone (4.4 mg.kg-1) to three ponies. It was concluded that drug uptake by and release from equine ingesta and digesta were probably adsorptive and desorptive processes. The clinical significance of the findings for the use of NSAIDs in equine medicine was considered.
Publication Date: 1988-01-01 PubMed ID: 3375588 The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research investigates how the drug phenylbutazone, and other related non-steroidal anti-inflammatory drugs (NSAIDs), interact with horse feed and digestive materials, both in controlled laboratory conditions and in the living organism. This understanding can provide insights into the effectiveness and behavior of such drugs in equine healthcare.
Research Methodology
- The research incorporated both in vitro (outside of a living organism) and in vivo (within a living organism) studies.
- In the in vitro experiments, binding of phenylbutazone to chopped hay and powdered pony nuts in buffer solutions was tested.
- The influence of time, concentration, and pH on drug binding were analyzed.
- In comparison to phenylbutazone, other NSAIDs like meclofenamate and flunixin were also tested for their level of binding to hay.
- The in vivo part of the research involved collecting digesta (digested or partially digested food in the intestines) from eight different regions of the horse’s gastrointestinal tract and analyzing the level of phenylbutazone binding to them.
- Ponies were administered orally with phenylbutazone to further understand its absorption and distribution in the equine digestive system.
Key Findings
- It was found that phenylbutazone binding to horse feed materials in vitro was dependent on various factors: its binding increased with time, decreased with concentration, and varied with the pH of the buffer solution.
- When compared to phenylbutazone, other NSAIDs like meclofenamate and flunixin showed lesser degrees of binding to hay.
- In vivo, phenylbutazone was found to bind extensively to digesta from various parts of the digestive tract. The highest percentage of binding was seen in the stomach (99.6%) and the lowest in the duodenum (80.0%).
- Phenylbutazone administered orally to ponies was also found to bind with the digesta.
Conclusion and Clinical Significance
- The researchers concluded that the absorption and distribution of phenylbutazone in the equine digestive system likely involve adsorptive and desorptive processes.
- Understanding drug interaction with horse feed and digesta can help optimize drug effectiveness and potentially minimize side effects in equine medicine. The study sheds light on the variable binding and absorption principles of NSAIDs, which could influence decisions about drug selection, dosage, and administration in equine healthcare.
Cite This Article
APA
Lees P, Taylor JB, Higgins AJ, Sedgwick AD.
(1988).
In vitro and in vivo binding of phenylbutazone and related drugs to equine feeds and digesta.
Res Vet Sci, 44(1), 50-56.
Publication
Researcher Affiliations
- Department of Physiology, Royal Veterinary College, North Mymms, Hatfield, Hertfordshire.
MeSH Terms
- Animal Feed
- Animals
- Gastrointestinal Contents / analysis
- Horses / metabolism
- Intestinal Absorption
- Phenylbutazone / analysis
- Phenylbutazone / metabolism
Citations
This article has been cited 4 times.- Ekstrand C, Michanek P, Hernlund E, Gehring R, Spjut K, Salomonsson M. Differences in Plasma Exposure of Cannabidiol and Cannabidiolic Acid Following Oral Administration to Horses. J Vet Pharmacol Ther 2026 Jan;49(1):22-32.
- Mercer MA, Davis JL, McKenzie HC. The Clinical Pharmacology and Therapeutic Evaluation of Non-Steroidal Anti-Inflammatory Drugs in Adult Horses. Animals (Basel) 2023 May 10;13(10).
- Kreuder AJ, Coetzee JF, Wulf LW, Schleining JA, KuKanich B, Layman LL, Plummer PJ. Bioavailability and pharmacokinetics of oral meloxicam in llamas. BMC Vet Res 2012 Jun 21;8:85.
- Lees P. Pharmacology of drugs used to treat osteoarthritis in veterinary practice. Inflammopharmacology 2003;11(4):385-99.
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