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In Vitro Generated Equine Hepatic-Like Progenitor Cells as a Novel Potent Cell Pool for Equine Metabolic Syndrome (EMS) Treatment.
Abstract: Equine metabolic syndrome (EMS) is recognized as one of the leading cause of health threatening in veterinary medicine worldwide. Recently, PTP1B inhibition has been proposed as an interesting strategy for liver insulin resistance reversion in both equines and humans, however as being a multifactorial disease, proper management of EMS horses further necessities additional interventional approaches aiming at repairing and restoring liver functions. In this study, we hypothesized that in vitro induction of Eq_ASCs hepatogenic differentiation will generate a specialized liver progenitor-like cell population exhibiting similar phenotypic characteristics and regenerative potential as native hepatic progenitor cells. Our obtained data demonstrated that Eq_ASCs-derived liver progenitor cells (Eq_HPCs) displayed typical flattened polygonal morphology with packed fragmented mitochondrial net, lowered mesenchymal CD105 and CD90 surface markers expression, and significant high expression levels of specific hepatic lineage genes including PECAM-1, ALB, AFP and HNF4A. therewith, generated Eq_HPCs exhibited potentiated stemness and pluripotency markers expression (NANOG, SOX-2 and OCT-4). Hence, in vitro generation of hepatic progenitor-like cells retaining high differentiation capacity represents a promising new approach for the establishment of cell-based targeted therapies for the restoration of proper liver functions in EMS affected horses.
© 2023. The Author(s).
Publication Date: 2023-01-20 PubMed ID: 36658383PubMed Central: PMC10185601DOI: 10.1007/s12015-023-10507-3Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigates the use of lab-grown liver cells from horses to treat equine metabolic syndrome (EMS), a common disease that affects the liver function of horses. This approach involves stimulating specific cells to transform into liver-like cells and target liver regeneration, which could provide new strategies for treatment.
Objective and Hypothesis
- The researchers were aiming to find novel ways to manage equine metabolic syndrome (EMS), a common condition in horses that poses a serious health risk.
- The hypothesized method involved stimulating equine adipose-derived stem cells (Eq_ASCs) in the lab to differentiate into liver progenitor-like cells, which would have similar features and regeneration capabilities as natural liver cells.
Methods and Experimental Approach
- The Eq_ASCs were induced to differentiate into liver progenitor cells (Eq_HPCs).
- The researchers closely observed these Eq_HPCs, examining their shape, the expression of mesenchymal markers (CD105 and CD90), and their mitochondrial structure to ensure they aligned with naturally occurring liver cells.
Findings and Results
- The data revealed that the artificially generated liver cells demonstrated typical traits of actual liver cells, such as specific flattened polygonal shape and packed fragmented mitochondrial structure.
- The cells presented low expression levels of mesenchymal markers, which are certain proteins usually expressed on the cell surface, aligning them with the typical appearance of liver cells.
- They also exhibited high expression levels of genes specific to liver lineage—PECAM-1, ALB, AFP and HNF4A.
- These cells showed potentiated expression of markers related to stemness and pluripotency (NANOG, SOX-2, and OCT-4), indicating a high potential for differentiation.
Conclusion and Implications
- The findings suggest that artificially generating hepatic progenitor-like cells with a high differentiation capacity is a promising approach for future EMS treatments.
- This presents potential opportunities for cell-based targeted therapies aimed at restoring liver function in horses affected by EMS.
Cite This Article
APA
Marycz K, Bourebaba N, Serwotka-Suszczak A, Mularczyk M, Galuppo L, Bourebaba L.
(2023).
In Vitro Generated Equine Hepatic-Like Progenitor Cells as a Novel Potent Cell Pool for Equine Metabolic Syndrome (EMS) Treatment.
Stem Cell Rev Rep, 19(4), 1124-1134.
https://doi.org/10.1007/s12015-023-10507-3 Publication
Researcher Affiliations
- International Institute of Translational Medicine, Jesionowa 11, Malin, 55-114, Wisznia Mau0142a, Poland. kmmarycz@ucdavis.edu.
- Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, Davis, CA, 95516, USA. kmmarycz@ucdavis.edu.
- Department of Experimental Biology, Faculty of Biology and Animal Science, Wrocu0142aw University of Environmental and Life Sciences, Norwida 27B, 50-375, Wrocu0142aw, Poland.
- Department of Experimental Biology, Faculty of Biology and Animal Science, Wrocu0142aw University of Environmental and Life Sciences, Norwida 27B, 50-375, Wrocu0142aw, Poland.
- International Institute of Translational Medicine, Jesionowa 11, Malin, 55-114, Wisznia Mau0142a, Poland.
- Department of Experimental Biology, Faculty of Biology and Animal Science, Wrocu0142aw University of Environmental and Life Sciences, Norwida 27B, 50-375, Wrocu0142aw, Poland.
- Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, Davis, CA, 95516, USA.
- Department of Experimental Biology, Faculty of Biology and Animal Science, Wrocu0142aw University of Environmental and Life Sciences, Norwida 27B, 50-375, Wrocu0142aw, Poland. lynda.bourebaba@upwr.edu.pl.
MeSH Terms
- Humans
- Horses
- Animals
- Metabolic Syndrome / therapy
- Metabolic Syndrome / metabolism
- Mesenchymal Stem Cells
- Stem Cells / metabolism
- Insulin Resistance
- Liver / metabolism
Conflict of Interest Statement
Not applicable.
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