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Journal of animal physiology and animal nutrition2016; 100(5); 828-835; doi: 10.1111/jpn.12448

Incorporation of sunflower oil or linseed oil in equine compound feedstuff: 1 Effects on haematology and on fatty acids profiles in the red blood cells membranes.

Abstract: Eight trained horses (6 mares - 2 geldings, 6 Selle Français, 2 Trotteur Français, 12 ± 5.8 years old, 538 ± 72.5 kg) were offered three diets to potentially affect haematology and the fatty acids (FA) profiles in red blood cells (RBC) membranes. The control diet was composed of 50% hay and 50% concentrate containing mainly rolled barley (48%) and whole spelt (48%). In the case of sunflower oil diet, sunflower oil (62.0% of α-linoleic acid, LA) was incorporated at a rate of 8% and substituted by an equal proportion of barley. In the linseed oil diet, first cold-pressed linseed oil (56.0% of α-linolenic acid, ALA) was utilised at a similar incorporation rate of 8%. The experimental design consisted of three 3 × 3 latin squares with one being incomplete. Each period lasted 8 weeks. On average, the total feed intake (straw excluded) was 6.2 kg/day and the oil intake 0.278 kg/day. The oils significantly increased the concentrations of RBC, haemoglobin and haematocrit. The oils had no significant impact on the haematology profiles except that platelets tended to decrease in both oil-based diets. The most abundant FA in the RBC membranes of the control diet samples were in the decreasing order LA, C18:1n9-7, C18:0, C16:0 and the arachidonic acid (ARA) respectively. The sunflower oil supplementation slightly increased the amount of LA (36.23 vs. 34.72 mg/dl, p = 0.55) and C22:4n-6 (0.21 vs. 0.09 mg/dl, p = 0.22), while the decrease was observed in case of other FA (C16:1n-7, 1.08 vs. 1.42 mg/dl, p = 0.03), C20:3n-6 (0.22 vs. 0.31 mg/dl, p = 0.02), and ARA (1.17 vs. 1.63 mg/dl, p = 0.08). Linseed oil induced similar effects in the n-6 series FA profiles. In the context of practical applications, our results show that linseed oil incorporation in the diet could improve the haematology and the n-3 FA profiles potentially leading to an increased performance.
Publication Date: 2016-04-14 PubMed ID: 27080721DOI: 10.1111/jpn.12448Google Scholar: Lookup
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  • Clinical Trial
  • Journal Article

Summary

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The research examines the effects of substituting traditional horse feed with sunflower and linseed oil on horse’s blood composition and the fatty acid profile of red blood cells. The study revealed that the incorporation of oils into the diet increased parameters like red blood cell count, hemoglobin concentration, and hematocrit value, while the fatty acids in the red blood cell membranes were affected differently by each oil.

Methods Used in the Study

  • An experiment involving eight trained horses was carried out to study the impact of dietary changes on their haematology and fatty acids profiles.
  • The horses were offered threedifferent diets – a traditional diet constituting hay and concentrate, a diet incorporating sunflower oil, and another incorporating linseed oil.
  • The oils were substituted for an equal proportion of barley in the feedstuffs. Sunflower oil and linseed oil were used since they are rich in α-linoleic acid and α-linolenic acid respectively. Each oil was incorporated at a rate of 8% in the respective diet.
  • The experimental design was three 3×3 Latin (cyclical) squares, with one being incomplete. Each period lasted 8 weeks.

Results of the Experiment

  • The findings indicate that the introduction of either oil into the diet significantly increased the concentration of red blood cells, haemoglobin, and haematocrit – major parameters in haematology profiling.
  • On the other hand, the incorporation of these oils did not have any significant effect on the haematology profiles except for platelet count, which showed a decreasing trend in both oil-based diets.
  • Other effects of the oil supplementation were observed in the fatty acids present in red blood cell membranes. The study determined the most abundant fatty acids present in the traditional diet samples and found that the presence of these was altered by the oils.
  • While sunflower oil increased the amounts of LA and C22:4n-6 fatty acids, linseed oil induced similar changes in the n-6 series fatty acid profiles. Linseed oil also showed an ability to improve the haematology and the n-3 fatty acid profile which might result in increased performance.

Conclusion

  • The results suggest that the incorporation of these oils could potentially benefit horses’ health and performance by improving haematological parameters and changing fatty acid profiles.
  • However, more research might be needed in this area, especially to understand the long-term impacts of such diet modifications on horse health and deportment.

Cite This Article

APA
Patoux S, Istasse L. (2016). Incorporation of sunflower oil or linseed oil in equine compound feedstuff: 1 Effects on haematology and on fatty acids profiles in the red blood cells membranes. J Anim Physiol Anim Nutr (Berl), 100(5), 828-835. https://doi.org/10.1111/jpn.12448

Publication

ISSN: 1439-0396
NlmUniqueID: 101126979
Country: Germany
Language: English
Volume: 100
Issue: 5
Pages: 828-835

Researcher Affiliations

Patoux, S
  • Nutrition Unit, Veterinary Medicine Faculty, University of Liège, Liège, Belgium. spatoux@ulg.ac.be.
Istasse, L
  • Nutrition Unit, Veterinary Medicine Faculty, University of Liège, Liège, Belgium.

MeSH Terms

  • Animal Feed / analysis
  • Animal Nutritional Physiological Phenomena
  • Animals
  • Cell Membrane / chemistry
  • Cell Membrane / metabolism
  • Cross-Over Studies
  • Diet / veterinary
  • Erythrocytes / metabolism
  • Fatty Acids / blood
  • Female
  • Horses / blood
  • Horses / physiology
  • Linseed Oil / administration & dosage
  • Linseed Oil / chemistry
  • Linseed Oil / pharmacology
  • Male
  • Plant Oils / administration & dosage
  • Plant Oils / chemistry
  • Plant Oils / pharmacology
  • Sunflower Oil

Citations

This article has been cited 1 times.
  1. Saastamoinen M, Särkijärvi S. Effect of Linseed (Linum usitatissimum) Groats-Based Mixed Feed Supplements on Diet Nutrient Digestibility and Blood Parameters of Horses. Animals (Basel) 2020 Feb 10;10(2).
    doi: 10.3390/ani10020272pubmed: 32050686google scholar: lookup