FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Stem Cell Rev Rep / Induced pluripotent stem cell lines derived from equine fibr...
Stem cell reviews and reports2011; 7(3); 693-702; doi: 10.1007/s12015-011-9239-5

Induced pluripotent stem cell lines derived from equine fibroblasts.

Abstract: The domesticated horse represents substantial value for the related sports and recreational fields, and holds enormous potential as a model for a range of medical conditions commonly found in humans. Most notable of these are injuries to muscles, tendons, ligaments and joints. Induced pluripotent stem (iPS) cells have sparked tremendous hopes for future regenerative therapies of conditions that today are not possible to cure. Equine iPS (EiPS) cells, in addition to bringing promises to the veterinary field, open up the opportunity to utilize horses for the validation of stem cell based therapies before moving into the human clinical setting. In this study, we report the generation of iPS cells from equine fibroblasts using a piggyBac (PB) transposon-based method to deliver transgenes containing the reprogramming factors Oct4, Sox2, Klf4 and c-Myc, expressed in a temporally regulated fashion. The established iPS cell lines express hallmark pluripotency markers, display a stable karyotype even during long-term culture, and readily form complex teratomas containing all three embryonic germ layer derived tissues upon in vivo grafting into immunocompromised mice. Our EiPS cell lines hold the promise to enable the development of a whole new range of stem cell-based regenerative therapies in veterinary medicine, as well as aid the development of preclinical models for human applications. EiPS cell could also potentially be used to revive recently extinct or currently threatened equine species.
Get Full Text
Publication Date: 2011-02-25 PubMed ID: 21347602PubMed Central: PMC3137777DOI: 10.1007/s12015-011-9239-5Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper describes the creation of pluripotent stem cells from horse fibroblasts. These cells have the potential to aid in the development of new regenerative therapies in veterinary medicine and could also be used in preclinical models for human applications.

Background

  • The research focuses on the significant value of the domesticated horse in sports, recreational fields, and as a model for studying a range of medical conditions commonly found in humans, particularly injuries to muscles, tendons, ligaments, and joints.
  • Induced pluripotent stem (iPS) cells have sparked significant interest in regenerative therapies that could potentially cure conditions that are currently incurable.
  • By creating equine iPS (EiPS) cells, the researchers aim to not only benefit the veterinary field but also validate stem cell-based therapies before moving into human clinical trials.

Methodology

  • The study involved the generation of iPS cells from horse fibroblasts. This was done using a piggyBac (PB) transposon-based method to deliver transgenes containing the reprogramming factors Oct4, Sox2, Klf4, and c-Myc, which were expressed in a temporally regulated way.

Findings

  • They successfully established iPS cell lines which expressed hallmark pluripotency markers. These cells display a stable karyotype even during long-term culture and they readily form complex teratomas comprising all three embryonic germ layer-derived tissues upon in vivo grafting into immunocompromised mice.
  • The study concludes that these EiPS cell lines hold great promise in advancing the development of stem cell-based regenerative therapies in veterinary medicine.
  • Moreover, they could aid in the development of preclinical models for human applications. Additionally, it is suggested that EiPS cells could potentially be used to revive recently extinct or currently threatened horse species.

Cite This Article

APA
Nagy K, Sung HK, Zhang P, Laflamme S, Vincent P, Agha-Mohammadi S, Woltjen K, Monetti C, Michael IP, Smith LC, Nagy A. (2011). Induced pluripotent stem cell lines derived from equine fibroblasts. Stem Cell Rev Rep, 7(3), 693-702. https://doi.org/10.1007/s12015-011-9239-5

Publication

Stem cell reviews and reports
ISSN: 2629-3277
NlmUniqueID: 101752767
Country: United States
Language: English
Volume: 7
Issue: 3
Pages: 693-702

Researcher Affiliations

Nagy, Kristina
  • Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada.
Sung, Hoon-Ki
    Zhang, Puzheng
      Laflamme, Simon
        Vincent, Patrick
          Agha-Mohammadi, Siamak
            Woltjen, Knut
              Monetti, Claudio
                Michael, Iacovos Prodromos
                  Smith, Lawrence Charles
                    Nagy, Andras

                      MeSH Terms

                      • Animals
                      • Cell Culture Techniques
                      • Cell Line
                      • Cell- and Tissue-Based Therapy / methods
                      • Cells, Cultured
                      • Cellular Reprogramming
                      • Fibroblasts / cytology
                      • Fibroblasts / physiology
                      • Horses
                      • Humans
                      • Induced Pluripotent Stem Cells / cytology
                      • Induced Pluripotent Stem Cells / physiology
                      • Kruppel-Like Factor 4
                      • Mice
                      • Regeneration
                      • Transgenes

                      Conflict of Interest Statement

                      The authors declare no potential conflicts of interest.

                      References

                      This article includes 31 references
                      1. Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos.. Nature 1981 Jul 9;292(5819):154-6.
                        doi: 10.1038/292154a0pubmed: 7242681google scholar: lookup
                      2. Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.. Proc Natl Acad Sci U S A 1981 Dec;78(12):7634-8.
                        doi: 10.1073/pnas.78.12.7634pmc: PMC349323pubmed: 6950406google scholar: lookup
                      3. Ying QL, Wray J, Nichols J, Batlle-Morera L, Doble B, Woodgett J, Cohen P, Smith A. The ground state of embryonic stem cell self-renewal.. Nature 2008 May 22;453(7194):519-23.
                        doi: 10.1038/nature06968pmc: PMC5328678pubmed: 18497825google scholar: lookup
                      4. Buehr M, Meek S, Blair K, Yang J, Ure J, Silva J, McLay R, Hall J, Ying QL, Smith A. Capture of authentic embryonic stem cells from rat blastocysts.. Cell 2008 Dec 26;135(7):1287-98.
                        doi: 10.1016/j.cell.2008.12.007pubmed: 19109897google scholar: lookup
                      5. Demers SP, Smith LC. Derivation, culture, and in vivo developmental capacity of embryonic cell lines from rat blastocysts.. Methods Mol Biol 2010;597:179-88.
                        doi: 10.1007/978-1-60327-389-3_13pubmed: 20013234google scholar: lookup
                      6. Li X, Zhou SG, Imreh MP, Ahrlund-Richter L, Allen WR. Horse embryonic stem cell lines from the proliferation of inner cell mass cells.. Stem Cells Dev 2006 Aug;15(4):523-31.
                        doi: 10.1089/scd.2006.15.523pubmed: 16978056google scholar: lookup
                      7. Saito S, Ugai H, Sawai K, Yamamoto Y, Minamihashi A, Kurosaka K, Kobayashi Y, Murata T, Obata Y, Yokoyama K. Isolation of embryonic stem-like cells from equine blastocysts and their differentiation in vitro.. FEBS Lett 2002 Nov 20;531(3):389-96.
                        doi: 10.1016/S0014-5793(02)03550-0pubmed: 12435581google scholar: lookup
                      8. Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells.. Nature 2007 Jul 19;448(7151):313-7.
                        doi: 10.1038/nature05934pubmed: 17554338google scholar: lookup
                      9. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.. Cell 2006 Aug 25;126(4):663-76.
                        doi: 10.1016/j.cell.2006.07.024pubmed: 16904174google scholar: lookup
                      10. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.. Cell 2007 Nov 30;131(5):861-72.
                        doi: 10.1016/j.cell.2007.11.019pubmed: 18035408google scholar: lookup
                      11. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA. Induced pluripotent stem cell lines derived from human somatic cells.. Science 2007 Dec 21;318(5858):1917-20.
                        doi: 10.1126/science.1151526pubmed: 18029452google scholar: lookup
                      12. Liu H, Zhu F, Yong J, Zhang P, Hou P, Li H, Jiang W, Cai J, Liu M, Cui K, Qu X, Xiang T, Lu D, Chi X, Gao G, Ji W, Ding M, Deng H. Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts.. Cell Stem Cell 2008 Dec 4;3(6):587-90.
                        doi: 10.1016/j.stem.2008.10.014pubmed: 19041774google scholar: lookup
                      13. Liao J, Cui C, Chen S, Ren J, Chen J, Gao Y, Li H, Jia N, Cheng L, Xiao H, Xiao L. Generation of induced pluripotent stem cell lines from adult rat cells.. Cell Stem Cell 2009 Jan 9;4(1):11-5.
                        doi: 10.1016/j.stem.2008.11.013pubmed: 19097959google scholar: lookup
                      14. Shimada H, Nakada A, Hashimoto Y, Shigeno K, Shionoya Y, Nakamura T. Generation of canine induced pluripotent stem cells by retroviral transduction and chemical inhibitors.. Mol Reprod Dev 2010 Jan;77(1):2.
                        doi: 10.1002/mrd.21117pubmed: 19890968google scholar: lookup
                      15. Esteban MA, Xu J, Yang J, Peng M, Qin D, Li W, Jiang Z, Chen J, Deng K, Zhong M, Cai J, Lai L, Pei D. Generation of induced pluripotent stem cell lines from Tibetan miniature pig.. J Biol Chem 2009 Jun 26;284(26):17634-40.
                        doi: 10.1074/jbc.M109.008938pmc: PMC2719402pubmed: 19376775google scholar: lookup
                      16. Ezashi T, Telugu BP, Alexenko AP, Sachdev S, Sinha S, Roberts RM. Derivation of induced pluripotent stem cells from pig somatic cells.. Proc Natl Acad Sci U S A 2009 Jul 7;106(27):10993-8.
                        doi: 10.1073/pnas.0905284106pmc: PMC2698893pubmed: 19541600google scholar: lookup
                      17. Wu Y, Zhang Y, Mishra A, Tardif SD, Hornsby PJ. Generation of induced pluripotent stem cells from newborn marmoset skin fibroblasts.. Stem Cell Res 2010 May;4(3):180-8.
                        doi: 10.1016/j.scr.2010.02.003pmc: PMC2875323pubmed: 20363201google scholar: lookup
                      18. Honda A, Hirose M, Hatori M, Matoba S, Miyoshi H, Inoue K, Ogura A. Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine.. J Biol Chem 2010 Oct 8;285(41):31362-9.
                        doi: 10.1074/jbc.M110.150540pmc: PMC2951210pubmed: 20670936google scholar: lookup
                      19. Nathan S, Das De S, Thambyah A, Fen C, Goh J, Lee EH. Cell-based therapy in the repair of osteochondral defects: a novel use for adipose tissue.. Tissue Eng 2003 Aug;9(4):733-44.
                        doi: 10.1089/107632703768247412pubmed: 13678450google scholar: lookup
                      20. Taylor SE, Smith RK, Clegg PD. Mesenchymal stem cell therapy in equine musculoskeletal disease: scientific fact or clinical fiction?. Equine Vet J 2007 Mar;39(2):172-80.
                        doi: 10.2746/042516407X180868pubmed: 17378447google scholar: lookup
                      21. Frisbie DD, Smith RK. Clinical update on the use of mesenchymal stem cells in equine orthopaedics.. Equine Vet J 2010 Jan;42(1):86-9.
                        doi: 10.2746/042516409X477263pubmed: 20121921google scholar: lookup
                      22. Wilke MM, Nydam DV, Nixon AJ. Enhanced early chondrogenesis in articular defects following arthroscopic mesenchymal stem cell implantation in an equine model.. J Orthop Res 2007 Jul;25(7):913-25.
                        doi: 10.1002/jor.20382pubmed: 17405160google scholar: lookup
                      23. Cohen S, Leshansky L, Zussman E, Burman M, Srouji S, Livne E, Abramov N, Itskovitz-Eldor J. Repair of full-thickness tendon injury using connective tissue progenitors efficiently derived from human embryonic stem cells and fetal tissues.. Tissue Eng Part A 2010 Oct;16(10):3119-37.
                        doi: 10.1089/ten.tea.2009.0716pubmed: 20486794google scholar: lookup
                      24. Kim D, Kim CH, Moon JI, Chung YG, Chang MY, Han BS, Ko S, Yang E, Cha KY, Lanza R, Kim KS. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins.. Cell Stem Cell 2009 Jun 5;4(6):472-6.
                        doi: 10.1016/j.stem.2009.05.005pmc: PMC2705327pubmed: 19481515google scholar: lookup
                      25. Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S. Generation of mouse induced pluripotent stem cells without viral vectors.. Science 2008 Nov 7;322(5903):949-53.
                        doi: 10.1126/science.1164270pubmed: 18845712google scholar: lookup
                      26. Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K. Induced pluripotent stem cells generated without viral integration.. Science 2008 Nov 7;322(5903):945-9.
                        doi: 10.1126/science.1162494pmc: PMC3987909pubmed: 18818365google scholar: lookup
                      27. Zhou H, Wu S, Joo JY, Zhu S, Han DW, Lin T, Trauger S, Bien G, Yao S, Zhu Y, Siuzdak G, Schöler HR, Duan L, Ding S. Generation of induced pluripotent stem cells using recombinant proteins.. Cell Stem Cell 2009 May 8;4(5):381-4.
                        doi: 10.1016/j.stem.2009.04.005pmc: PMC10182564pubmed: 19398399google scholar: lookup
                      28. Wang W, Lin C, Lu D, Ning Z, Cox T, Melvin D, Wang X, Bradley A, Liu P. Chromosomal transposition of PiggyBac in mouse embryonic stem cells.. Proc Natl Acad Sci U S A 2008 Jul 8;105(27):9290-5.
                        doi: 10.1073/pnas.0801017105pmc: PMC2440425pubmed: 18579772google scholar: lookup
                      29. Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K. Virus-free induction of pluripotency and subsequent excision of reprogramming factors.. Nature 2009 Apr 9;458(7239):771-5.
                        doi: 10.1038/nature07864pmc: PMC2667910pubmed: 19252477google scholar: lookup
                      30. Lin T, Ambasudhan R, Yuan X, Li W, Hilcove S, Abujarour R, Lin X, Hahm HS, Hao E, Hayek A, Ding S. A chemical platform for improved induction of human iPSCs.. Nat Methods 2009 Nov;6(11):805-8.
                        doi: 10.1038/nmeth.1393pmc: PMC3724527pubmed: 19838168google scholar: lookup
                      31. Paris DB, Stout TA. Equine embryos and embryonic stem cells: defining reliable markers of pluripotency.. Theriogenology 2010 Sep 1;74(4):516-24.
                        doi: 10.1016/j.theriogenology.2009.11.020pubmed: 20071015google scholar: lookup

                      Citations

                      This article has been cited 98 times.
                      Subscribe to our newsletter
                      Join 99,383 horse owners like you who receive our email updates on horse health and nutrition.