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Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint?
Abstract: The effects of recombinant interleukin-1β (rIL-1β) have been described for the middle carpal joint (MCJ). However, we are unaware of any studies that have described the cytological response of the tibiotarsal joint (TTJ) to rIL-1β or compared the clinical and cytological responses of the MCJ to the TTJ following the administration of intra-articular rIL-1β. Such information is critical for researchers planning to use rIL-1β to create acute synovitis models in horses. To compare the clinical and cytological responses of the MCJ to the TTJ following administration of intra-articular rIL-1β. Twelve horses were used for the study. Eight horses received 75 ng of rIL-1β into the MCJ and four horses received 75 ng of rIL-1β into the TTJ. Clinical and cytological outcome parameters including lameness, joint circumference, joint effusion score, total nucleated cell count, cellular differentials, C-reactive protein, and prostaglandin-E2 concentrations were determined at baseline and multiple post-treatment time points over a 336 h period (2 weeks). Recombinant IL-1β administered into the TTJ resulted in a significantly greater respiratory rate at 24 h and heart rate at 12 h when compared to rIL-1β administered into the MCJ. In addition, the TTJ had a significantly greater increase in joint circumference at 24 post-injection hour (PIH) and subjective effusion grade at 24 PIH and 336 PIH. The MCJ had significantly higher total protein concentration at 6 PIH, and a significantly higher NCC at 24 and 72 PIH when compared to the TTJ. Conversely, the TTJ had significantly higher neutrophilic infiltration than the MCJ at 6 PIH and 168 PIH. This study establishes that the same intra-articular dose of rIL-1 β elicits significantly different clinical and cytological responses in the MCJ compared to the TTJ in the equine model of intra-articular synovitis. In addition, clinical and cytological evidence of synovitis may persist up to or >1 week following intra-articular administration of rIL-1 β.
Publication Date: 2018-08-31 PubMed ID: 30234134PubMed Central: PMC6127273DOI: 10.3389/fvets.2018.00208Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This study investigates the different response of two horse joints, the Middle Carpal Joint (MCJ) and the Tibiotarsal Joint (TTJ), to a dose of Interleukin-1 Beta (rIL-1β), a protein causing inflammation. The results show that the same dose can lead to notably different clinical and cytological effects in each joint.
Objective of the Research
- The main aim of this research was to study and compare the reactions of two particular horse joints – the Middle Carpal Joint (MCJ) and the Tibiotarsal Joint (TTJ) – when exposed to recombinant Interleukin-1β (rIL-1β). rIL-1β is a protein known to induce inflammation, and the researchers were seeking to understand if the two joints responded differently to the same dose.
Methodology
- Twelve horses participated in the study, with eight horses receiving 75 ng of rIL-1β into the MCJ and four injected with the same dose into the TTJ.
- Clinical and cytological parameters including lameness, joint circumference, joint effusion score, total cell count, different types of cells, C-reactive protein, and prostaglandin-E2 concentrations were all gauged at baseline and multiple post-treatment points over a two-week period.
Results
- The study found that the TTJ responded differently to the rIL-1β when compared to the MCJ. Characteristically, there was a greater respiratory rate at 24 hours and a higher heart rate at 12 hours when rIL-1β was administered into the TTJ.
- An increase in joint circumference and subjective effusion grade at 24 hours and 336 hours post-injection was observed in the TTJ compared to the MCJ.
- However, the MCJ showcased a higher total protein concentration at 6 hours post injection, and a higher NCC at 24 and 72 hours.
- The TTJ showed a significantly higher neutrophilic infiltration than the MCJ at 6 hours and 168 hours post injection.
Conclusion
- The results of this study demonstrate that the clinical and cytological responses can differ significantly between these two horse joints when the same dose of rIL-1β is administered. This means that inflammation can persist for a longer period in one joint than the other, which is highly significant for designing future treatments and studies.
Cite This Article
APA
Colbath AC, Dow SW, Hopkins LS, Phillips JN, McIlwraith CW, Goodrich LR.
(2018).
Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint?
Front Vet Sci, 5, 208.
https://doi.org/10.3389/fvets.2018.00208 Publication
Researcher Affiliations
- Department of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Department of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Department of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Department of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
References
This article includes 21 references
- Bird JL, Wells T, Platt D, Bayliss MT. IL-1 beta induces the degradation of equine articular cartilage by a mechanism that is not mediated by nitric oxide.. Biochem Biophys Res Commun 1997 Sep 8;238(1):81-5.
- DePuy T, Howard R, Keegan K, Wilson D, Kramer J, Cook JL, Childers MK. Effects of intra-articular botulinum toxin type A in an equine model of acute synovitis: a pilot study.. Am J Phys Med Rehabil 2007 Oct;86(10):777-83.
- Ross TN, Kisiday JD, Hess T, McIlwraith CW. Evaluation of the inflammatory response in experimentally induced synovitis in the horse: a comparison of recombinant equine interleukin 1 beta and lipopolysaccharide.. Osteoarthritis Cartilage 2012 Dec;20(12):1583-90.
- Tóth F, Schumacher J, Schramme MC, Hecht S. Effect of anesthetizing individual compartments of the stifle joint in horses with experimentally induced stifle joint lameness.. Am J Vet Res 2014 Jan;75(1):19-25.
- Svala E, Jin C, Rüetschi U, Ekman S, Lindahl A, Karlsson NG, Skiöldebrand E. Characterisation of lubricin in synovial fluid from horses with osteoarthritis.. Equine Vet J 2017 Jan;49(1):116-123.
- Svala E, Löfgren M, Sihlbom C, Rüetschi U, Lindahl A, Ekman S, Skiöldebrand E. An inflammatory equine model demonstrates dynamic changes of immune response and cartilage matrix molecule degradation in vitro.. Connect Tissue Res 2015;56(4):315-25.
- Nelson BB, King MR, Frisbie DD. Assessment of a novel equine tarsocrural experimental joint disease model using recombinant interleukin-1β and arthroscopic articular sampling of the medial malleolus of the tibia on the standing sedated horse.. Vet J 2017 Nov;229:54-59.
- Smith MD, Triantafillou S, Parker A, Youssef PP, Coleman M. Synovial membrane inflammation and cytokine production in patients with early osteoarthritis.. J Rheumatol 1997 Feb;24(2):365-71.
- Hussein MR, Fathi NA, El-Din AM, Hassan HI, Abdullah F, Al-Hakeem E, Backer EA. Alterations of the CD4(+), CD8 (+) T cell subsets, interleukins-1beta, IL-10, IL-17, tumor necrosis factor-alpha and soluble intercellular adhesion molecule-1 in rheumatoid arthritis and osteoarthritis: preliminary observations.. Pathol Oncol Res 2008 Sep;14(3):321-8.
- Frisbie DD, Ghivizzani SC, Robbins PD, Evans CH, McIlwraith CW. Treatment of experimental equine osteoarthritis by in vivo delivery of the equine interleukin-1 receptor antagonist gene.. Gene Ther 2002 Jan;9(1):12-20.
- Elsaid KA, Ubhe A, Shaman Z, D'Souza G. Intra-articular interleukin-1 receptor antagonist (IL1-ra) microspheres for posttraumatic osteoarthritis: in vitro biological activity and in vivo disease modifying effect.. J Exp Orthop 2016 Dec;3(1):18.
- Punzi L, Galozzi P, Luisetto R, Favero M, Ramonda R, Oliviero F, Scanu A. Post-traumatic arthritis: overview on pathogenic mechanisms and role of inflammation.. RMD Open 2016;2(2):e000279.
- Carmalt JL, Bell CD, Tatarniuk DM, Suri SS, Singh B, Waldner C. Comparison of the response to experimentally induced short-term inflammation in the temporomandibular and metacarpophalangeal joints of horses.. Am J Vet Res 2011 Dec;72(12):1586-91.
- Williams LB, Koenig JB, Black B, Gibson TW, Sharif S, Koch TG. Equine allogeneic umbilical cord blood derived mesenchymal stromal cells reduce synovial fluid nucleated cell count and induce mild self-limiting inflammation when evaluated in an lipopolysaccharide induced synovitis model.. Equine Vet J 2016 Sep;48(5):619-25.
- Carlson ER, Stewart AA, Carlson KL, Durgam SS, Pondenis HC. Effects of serum and autologous conditioned serum on equine articular chondrocytes treated with interleukin-1β.. Am J Vet Res 2013 May;74(5):700-5.
- Ownby SL, Fortuno LV, Au AY, Grzanna MW, Rashmir-Raven AM, Frondoza CG. Expression of pro-inflammatory mediators is inhibited by an avocado/soybean unsaponifiables and epigallocatechin gallate combination.. J Inflamm (Lond) 2014 Mar 28;11(1):8.
- Jacobsen S, Ladefoged S, Berg LC. Production of serum amyloid A in equine articular chondrocytes and fibroblast-like synoviocytes treated with proinflammatory cytokines and its effects on the two cell types in culture.. Am J Vet Res 2016 Jan;77(1):50-8.
- Ross-Jones TN, McIlwraith CW, Kisiday JD, Hess TM, Hansen DK, Black J. Influence of an n-3 long-chain polyunsaturated fatty acid-enriched diet on experimentally induced synovitis in horses.. J Anim Physiol Anim Nutr (Berl) 2016 Jun;100(3):565-77.
- Van Pelt RW. Characteristics of normal equine tarsal synovial fluid.. Can J Comp Med Vet Sci 1967 Dec;31(12):342-7.
- Harada A, Sekido N, Akahoshi T, Wada T, Mukaida N, Matsushima K. Essential involvement of interleukin-8 (IL-8) in acute inflammation.. J Leukoc Biol 1994 Nov;56(5):559-64.
- Tobe M, Ogura N, Abiko Y, Nagura H. Interleukin-1beta stimulates interleukin-8 production and gene expression in synovial cells from human temporomandibular joint.. J Oral Maxillofac Surg 2002 Jul;60(7):741-7.
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