Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint?
Abstract: The effects of recombinant interleukin-1β (rIL-1β) have been described for the middle carpal joint (MCJ). However, we are unaware of any studies that have described the cytological response of the tibiotarsal joint (TTJ) to rIL-1β or compared the clinical and cytological responses of the MCJ to the TTJ following the administration of intra-articular rIL-1β. Such information is critical for researchers planning to use rIL-1β to create acute synovitis models in horses. To compare the clinical and cytological responses of the MCJ to the TTJ following administration of intra-articular rIL-1β. Twelve horses were used for the study. Eight horses received 75 ng of rIL-1β into the MCJ and four horses received 75 ng of rIL-1β into the TTJ. Clinical and cytological outcome parameters including lameness, joint circumference, joint effusion score, total nucleated cell count, cellular differentials, C-reactive protein, and prostaglandin-E2 concentrations were determined at baseline and multiple post-treatment time points over a 336 h period (2 weeks). Recombinant IL-1β administered into the TTJ resulted in a significantly greater respiratory rate at 24 h and heart rate at 12 h when compared to rIL-1β administered into the MCJ. In addition, the TTJ had a significantly greater increase in joint circumference at 24 post-injection hour (PIH) and subjective effusion grade at 24 PIH and 336 PIH. The MCJ had significantly higher total protein concentration at 6 PIH, and a significantly higher NCC at 24 and 72 PIH when compared to the TTJ. Conversely, the TTJ had significantly higher neutrophilic infiltration than the MCJ at 6 PIH and 168 PIH. This study establishes that the same intra-articular dose of rIL-1 β elicits significantly different clinical and cytological responses in the MCJ compared to the TTJ in the equine model of intra-articular synovitis. In addition, clinical and cytological evidence of synovitis may persist up to or >1 week following intra-articular administration of rIL-1 β.
Publication Date: 2018-08-31 PubMed ID: 30234134PubMed Central: PMC6127273DOI: 10.3389/fvets.2018.00208Google Scholar: Lookup
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- Journal Article
Summary
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This study investigates the different response of two horse joints, the Middle Carpal Joint (MCJ) and the Tibiotarsal Joint (TTJ), to a dose of Interleukin-1 Beta (rIL-1β), a protein causing inflammation. The results show that the same dose can lead to notably different clinical and cytological effects in each joint.
Objective of the Research
- The main aim of this research was to study and compare the reactions of two particular horse joints – the Middle Carpal Joint (MCJ) and the Tibiotarsal Joint (TTJ) – when exposed to recombinant Interleukin-1β (rIL-1β). rIL-1β is a protein known to induce inflammation, and the researchers were seeking to understand if the two joints responded differently to the same dose.
Methodology
- Twelve horses participated in the study, with eight horses receiving 75 ng of rIL-1β into the MCJ and four injected with the same dose into the TTJ.
- Clinical and cytological parameters including lameness, joint circumference, joint effusion score, total cell count, different types of cells, C-reactive protein, and prostaglandin-E2 concentrations were all gauged at baseline and multiple post-treatment points over a two-week period.
Results
- The study found that the TTJ responded differently to the rIL-1β when compared to the MCJ. Characteristically, there was a greater respiratory rate at 24 hours and a higher heart rate at 12 hours when rIL-1β was administered into the TTJ.
- An increase in joint circumference and subjective effusion grade at 24 hours and 336 hours post-injection was observed in the TTJ compared to the MCJ.
- However, the MCJ showcased a higher total protein concentration at 6 hours post injection, and a higher NCC at 24 and 72 hours.
- The TTJ showed a significantly higher neutrophilic infiltration than the MCJ at 6 hours and 168 hours post injection.
Conclusion
- The results of this study demonstrate that the clinical and cytological responses can differ significantly between these two horse joints when the same dose of rIL-1β is administered. This means that inflammation can persist for a longer period in one joint than the other, which is highly significant for designing future treatments and studies.
Cite This Article
APA
Colbath AC, Dow SW, Hopkins LS, Phillips JN, McIlwraith CW, Goodrich LR.
(2018).
Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint?
Front Vet Sci, 5, 208.
https://doi.org/10.3389/fvets.2018.00208 Publication
Researcher Affiliations
- Department of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Department of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Department of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
- Department of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United States.
- Clinical Sciences, Colorado State University, Fort Collins, CO, United States.
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Citations
This article has been cited 4 times.- Wu J, Li H, Hu F, Luo P. Stevioside attenuates osteoarthritis via regulating Nrf2/HO-1/NF-κB pathway.. J Orthop Translat 2023 Jan;38:190-202.
- Garbin LC, Contino EK, Olver CS, Frisbie DD. A safety evaluation of allogeneic freeze-dried platelet-rich plasma or conditioned serum compared to autologous frozen products equivalents in equine healthy joints.. BMC Vet Res 2022 Apr 18;18(1):141.
- Watkins A, Fasanello D, Stefanovski D, Schurer S, Caracappa K, D'Agostino A, Costello E, Freer H, Rollins A, Read C, Su J, Colville M, Paszek M, Wagner B, Reesink H. Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models.. BMC Vet Res 2021 May 12;17(1):189.
- Colbath AC, Dow SW, Hopkins LS, Phillips JN, McIlwraith CW, Goodrich LR. Single and repeated intra-articular injections in the tarsocrural joint with allogeneic and autologous equine bone marrow-derived mesenchymal stem cells are safe, but did not reduce acute inflammation in an experimental interleukin-1β model of synovitis.. Equine Vet J 2020 Jul;52(4):601-612.
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