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Stem cells international2016; 2016; 7342379; doi: 10.1155/2016/7342379

Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching.

Abstract: Tendon and ligament pathologies are still a therapeutic challenge, due to the difficulty in restoring the complex extracellular matrix architecture and biomechanical strength. While progress is being made in cell-based therapies and tissue engineering approaches, comprehensive understanding of the fate of progenitor cells in tendon healing is still lacking. The aim of this study was to investigate the effect of decellularized tendon matrix and moderate cyclic stretching as natural stimuli which could potentially direct tenogenic fate. Equine adipose-derived mesenchymal stromal cells (MSC) were seeded on decellularized tendon matrix scaffolds. Mechanical stimulation was applied in a custom-made cyclic strain bioreactor. Assessment was performed 4 h, 8 h, and 24 h following mechanical stimulation. Scaffold culture induced cell alignment and changes in expression of tendon-related genes, although cell viability was decreased compared to monolayer culture. Short mechanical stimulation periods enhanced most of the scaffold-induced effects. Collagen 1A2 expression levels were decreased, while collagen 3A1 and decorin levels were increased. Tenascin-C and scleraxis expression showed an initial decrease but had increased 24 h after stimulation. The results obtained suggest that decellularized tendon matrix, supported by cyclic stretching, can induce tenogenic differentiation and the synthesis of tendon components important for matrix remodeling.
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Publication Date: 2016-08-18 PubMed ID: 27630718PubMed Central: PMC5007347DOI: 10.1155/2016/7342379Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study focuses on understanding how the application of a natural stimulus like decellularized tendon matrix and moderate cyclic stretching could potentially aid in tendon healing. The researchers discovered that decellularized tendon matrix with support from cyclic stretching can result in tenogenic differentiation and synthesis of tendon components crucial for matrix remodeling.

Objective and Need of the Study

  • The main objective of this investigation is to understand the effect of natural stimuli including a decellularized tendon matrix and moderate cyclic stretching on tendon healing.
  • Rationale behind the research is the challenge prevailing in treating tendon and ligament pathologies due to the difficulty in restoring the complex extracellular matrix architecture and biomechanical strength required for tendon healing.
  • There is a significant lack of comprehensive understanding of how progenitor cells behave during tendon healing which this study attempts to analyze.

Methodology

  • The research used Equine adipose-derived mesenchymal stromal cells (MSC) which were seeded on decellularized tendon matrix scaffolds.
  • Cyclic strain was applied using a custom-made bioreactor. This mechanical stimulation was applied for 4 h, 8 h, and 24 h duration to evaluate the impact of the stimulus on cells.

Observations and Conclusions

  • The scaffold culture process induced cell alignment as well as changes in the expression of tendon-associated genes. However, the cell viability noticed was decreased as compared to the traditional monolayer cell culture technique.
  • Short periods of mechanical stimulation enhanced most of the effects caused by the scaffold. For instance, expression levels of Collagen 1A2 were decreased while Collagen 3A1 and decorin levels witnessed an increase.
  • Expression of Tenascin-C and scleraxis showed an initial drop but exhibited an increase 24 h after stimulation.
  • The results imply that utilizing decellularized tendon matrix, augmented by cyclic stretching, can encourage tenogenic differentiation. This further leads to the synthesis of significant tendon components that contribute to matrix remodeling, highlighting a prospective approach to tendon healing.

Cite This Article

APA
Burk J, Plenge A, Brehm W, Heller S, Pfeiffer B, Kasper C. (2016). Induction of Tenogenic Differentiation Mediated by Extracellular Tendon Matrix and Short-Term Cyclic Stretching. Stem Cells Int, 2016, 7342379. https://doi.org/10.1155/2016/7342379

Publication

Stem cells international
ISSN: 1687-966X
NlmUniqueID: 101535822
Country: United States
Language: English
Volume: 2016
Pages: 7342379

Researcher Affiliations

Burk, Janina
  • Translational Centre for Regenerative Medicine, University of Leipzig, Philipp-Rosenthal-Strasse 55, 04103 Leipzig, Germany; Saxon Incubator for Clinical Translation, University of Leipzig, Philipp-Rosenthal-Strasse 55, 04103 Leipzig, Germany; Institute of Veterinary Physiology, University of Leipzig, An den Tierkliniken 7, 04103 Leipzig, Germany.
Plenge, Amelie
  • Large Animal Clinic for Surgery, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, Germany.
Brehm, Walter
  • Translational Centre for Regenerative Medicine, University of Leipzig, Philipp-Rosenthal-Strasse 55, 04103 Leipzig, Germany; Saxon Incubator for Clinical Translation, University of Leipzig, Philipp-Rosenthal-Strasse 55, 04103 Leipzig, Germany; Large Animal Clinic for Surgery, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, Germany.
Heller, Sandra
  • Translational Centre for Regenerative Medicine, University of Leipzig, Philipp-Rosenthal-Strasse 55, 04103 Leipzig, Germany; Department of Pathology and Laboratory Medicine, Tulane University, 1430 Tulane Avenue, New Orleans, LA 70112, USA.
Pfeiffer, Bastian
  • Large Animal Clinic for Surgery, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, Germany.
Kasper, Cornelia
  • Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Muthgasse 18, 1190 Vienna, Austria.

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This article has been cited 34 times.
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