Influence of haematological parameters on size of the advanced platelet-rich fibrin+ (A-PRF+) in the horse.

The research investigated the impact of specific blood parameters on the size of an advanced platelet-rich fibrin (A-PRF+) formation in horses, while also observing whether different centrifugation methods affected its production. The study findings were inconclusive in establishing any significant correlation between these blood parameters and the clot size.

Overview of the Study

• The study involved analysing the potential influence of haematological parameters (properties of one’s blood) on the size of advanced platelet-rich fibrin (A-PRF+) using equine (horse) samples.
• A-PRF+ is a highly concentrated platelet solution, noted for its higher cell numbers and looser clot structure compared to its counterpart, the leukocyte-PRF.
• The researchers specifically endeavoured to evaluate the practicality of generating A-PRF+ samples in field conditions and understand the connection between haematological parameters and the macroscopic and microscopic characteristics of the platelet-rich substance.

Methods Used in the Research

• The research used blood samples collected from twenty Standardbred horses aged between 3-7 years. These blood samples were harvested using glass tubes, free from anticoagulants and pre-heated at 37 °C.
• Following the collection, the blood samples were then centrifuged at 1300 rpm for 8 minutes using a fixed-angle centrifuge and a horizontal centrifuge and this was done under field conditions, in temperatures ranging 15-17 °C.
• Following centrifugation, the formed clots were measured and then put into the Wound Box® for 2-minute compression. The membranes were then measured and fixed with 10% formalin to be prepared for histological (tissue structure) examination.

Findings of the Study

• Researchers found that the overall clot size and surface did not significantly differ between sexes or the type of centrifuge used.
• Similarly, the team found no notable correlation between the haematological parameters and the size of the clot or membrane.
• In studying the clots, the research team observed both types of centrifugation led to a clot with a loose fibrin structure and cells that are evenly distributed throughout its composition.
• It was evident from the study that the method of heating the glass tubes enabled the successful extraction of A-PRF+ clots from all samples, regardless of the prevailing environmental temperature.

Future Directions

• The researchers suggest that subsequent studies should explore the potential influences of other blood molecules on the structure and dimensions of A-PRF+.