Injury induces a change in the functional characteristics of cells recovered from equine tendon.
Abstract: Injury initiates a repair process characterized by influx of fibroblasts and the rapid formation of fibrous scar tissue and subsequent tissue contraction. The response to injury and behavior of the different tendon fibroblast populations, however, has been poorly characterized. We hypothesized that the fibroblasts recovered from tendon with acute injury would exhibit different cell properties relating to adhesion, migration and tensegrity. To test this hypothesis we evaluated the ability of fibroblasts recovered from normal and injured equine superficial digital flexor tendons (SDFTs). The injured tendon-derived cells showed greater contraction of the collagen gel but poorer adhesion to pepsin-digested collagen, and migration over extracellular matrix proteins compared to normal SDFT-derived fibroblasts. Thus, the cells present within the tendon after injury display different behavior related to wound healing.
Publication Date: 2011-10-25 PubMed ID: 24833988PubMed Central: PMC4013992DOI: 10.1294/jes.22.57Google Scholar: Lookup
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- Journal Article
Summary
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The research article is about how injury changes the functional characteristics of cells in equine tendons.
Overview of the Research
- This research aimed to examine how injury induces changes in the behavior and properties of cells taken from horse tendons. This was primarily achieved by evaluating the functional characteristics of fibroblasts – a type of cell that plays a critical role in wound healing – that were recovered from both normal and injured tendons.
- The focus of the study was the superficial digital flexor tendons (SDFTs) in horses, a common site of injury.
Main Findings
- The team found out that injury invokes a repair process that includes the influx of fibroblasts (a cell type involved in wound repair) and the quick creation of fibrous scar tissue and ensuing tissue contraction.
- Upon evaluating the abilities of fibroblasts from regular and injured equine SDFTs, they discovered that fibroblasts from injured tendons showed more pronounced contraction of the collagen gel (a substance involved in healing and tissue regeneration), but they were less proficient at adhering to collagen digested by pepsin and migrating over the extracellular matrix proteins than the fibroblasts derived from normal tendons.
- These results unveil that the cells present in the tendon post-injury display different behaviors linked to wound healing. This shift in behavior could have implications for how efficiently wounds heal and may influence possible treatment strategies for tendon injuries.
Implications of the Research
- Understanding the behavior and properties of cells in the tendon after an injury is crucial as this could influence the treatment strategies for such injuries. Knowing that cells from injured tendons are less efficient at adhering to collagen and migrating over extracellular matrix proteins might suggest specific therapeutic interventions to enhance these processes and thus improve healing outcomes.
- Irrespective of whether the impact of this research is viewed in terms of its potential benefits to equine health or to humans (since tendons in humans and horses have similar properties), this study contributes crucial insights towards the understanding of how injury alters cell properties and behavior in tendons.
Cite This Article
APA
Kihara R, Kasashima Y, Arai K, Miyamoto Y.
(2011).
Injury induces a change in the functional characteristics of cells recovered from equine tendon.
J Equine Sci, 22(3), 57-60.
https://doi.org/10.1294/jes.22.57 Publication
Researcher Affiliations
- Division of Life Sciences, Graduate School of Humanities and Sciences, Ochanomizu University, Tokyo 112-8610, Japan.
- Clinical Science and Pathobiology Division, Equine Research Institute, Japan Racing Association, Utsunomiya, Tochigi 320-0856, Japan ; Department of Veterinary Clinical Sciences, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts, AL9 7TA, U.K.
- Department of Tissue Physiology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
- Division of Life Sciences, Graduate School of Humanities and Sciences, Ochanomizu University, Tokyo 112-8610, Japan.
References
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