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Cell and tissue research1976; 172(4); 447-454; doi: 10.1007/BF00220331

Insulin-, glucagon-, and somatostatin-immunoreactive endocrine cells in the equine pancreas.

Abstract: Equine pancreas was investigated with immunohistochemical methods to study the distribution of endocrine cells immunoreactive to anti-insulin, anti-glucagon, and anti-somatostatin. A-cells demonstrable by anti-glucagon are located in the center of Langerhans islets and frequently in the duct epithelium. Few A-cells are seen associated to acini. Anti-insulin reactive B-cells form a large zone around the center of the Langerhans islets in which some B-cells lie between exocrine cells and others, although few, are located in the duct epithelium. D-cells stained with anti-somatostatin serum form a discontinuous outermost zone around the Langerhans islets. In some islets the D-cells are also observed among the B-cells or between the border of A- and B-cells. Single D-cells are seen in the duct epithelium or between acinar cells. In younger horses, endocrine cells are more frequently associated in bulges of the duct system. The histotopographic relation between these endocrine cell types is discussed with respect to its functional significance.
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Publication Date: 1976-09-29 PubMed ID: 791497DOI: 10.1007/BF00220331Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper delves into the study of the distribution of certain endocrine cells within the equine (horse) pancreas, including cells that are reactive to insulin, glucagon, and somatostatin. The research used immunohistochemical methods, focusing on the relationship between these endocrine cell types in their specific locations and their functional significance.

Methodology

  • The research was carried out using immunohistochemical methods, a technique used to detect specific proteins in cells of a tissue section.
  • The researchers studied the cells in the equine pancreas that are reactive to substances such as insulin, glucagon, and somatostatin.

Key Findings

  • A-cells that were demonstrable by anti-glucagon were located at the center of the Langerhans islets and frequently within the duct epithelium.
  • There were only a few A-cells seen associated with acini (small exocrine glands).
  • B-cells reactive to anti-insulin formed a large zone around the center of the Langerhans islets and, while few were located in the duct epithelium, others laid between exocrine cells.
  • D-cells stained with anti-somatostatin serum formed an outermost zone around the Langerhans islets, in a discontinuous pattern. These D-cells were found among the intra-islet B-cells, at the border of A- and B-cells, and as single cells within the duct or acinar epithelium.
  • In younger horses, endocrine cells were more frequently grouped in the bulges of the duct system.

Discussion

  • The research discusses the histotopographic relationship between these endocrine cells and its functional significance.
  • This highlights the topographical relation between these cells and the understanding of the overall function and mechanism of the equine pancreas.

Significance of the Study

  • This study is essential in understanding the cell mechanics within the equine pancreas and provides crucial insight into the organ’s function and structure.
  • It opens doors for further research on equine pancreas function and associated pathologies for better management and treatment of related diseases.

Cite This Article

APA
Helmstaedter V, Feurle GE, Forssmann WG. (1976). Insulin-, glucagon-, and somatostatin-immunoreactive endocrine cells in the equine pancreas. Cell Tissue Res, 172(4), 447-454. https://doi.org/10.1007/BF00220331

Publication

Cell and tissue research
ISSN: 0302-766X
NlmUniqueID: 0417625
Country: Germany
Language: English
Volume: 172
Issue: 4
Pages: 447-454

Researcher Affiliations

Helmstaedter, V
    Feurle, G E
      Forssmann, W G

        MeSH Terms

        • Animals
        • Antigens
        • Fluorescent Antibody Technique
        • Glucagon / analysis
        • Horses / anatomy & histology
        • Insulin / analysis
        • Islets of Langerhans / cytology
        • Islets of Langerhans / immunology
        • Somatostatin / analysis
        • Somatostatin / immunology

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