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Home / Equine Research Bank / Am J Physiol / Interrelationship of Na, HCO3, and volatile fatty acid trans...
The American journal of physiology1977; 233(6); E469-E478; doi: 10.1152/ajpendo.1977.233.6.E469

Interrelationship of Na, HCO3, and volatile fatty acid transport by equine large intestine.

Abstract: No abstract available
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Publication Date: 1977-12-01 PubMed ID: 596440DOI: 10.1152/ajpendo.1977.233.6.E469Google Scholar: Lookup
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  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research conducted by Argenzio, Southworth, Low’s, and Stevens investigates the role of Sodium (Na), Bicarbonate (HCO3), and Volatile Fatty Acid (VFA) transport in the large intestine of horses, with the study using both in vitro and in vivo methods.

Research Objective

  • The purpose of this research was to examine the interrelationship between Na, HCO3, and volatile fatty acid (VFA) transport in the large intestine of horses. This study also aimed to determine the role of VFA in the movement of Na and HCO3 across the mucosa of the equine large intestine.

Methodology

  • To conduct the in vitro studies, tissues from the cecum and colon of mature Shetland ponies were used. These ponies were treated for intestinal parasites and fasted for 24 hours prior to tissue collection. After euthanasia, the tissues were collected and rinsed with equine bicarbonate Ringer solution. Then, they were submerged into oxygenated bicarbonate Ringer solution, and the mucosa was dissected free of underlying muscle.
  • For the in vivo studies, the researchers performed similar experiments but using live ponies.

Key Findings

  • The research found a significant difference in the rate of active Na absorption by the mucosa from different segments of the large intestine. For instance, the distal colon actively transported Na at approximately six times the rate of tissue from the proximal colon.
  • The presence of acetate, one type of VFA, significantly inhibited net Na transport by the cecal mucosa. It completely abolished net Na transport by the mucosa from the small (distal) colon.
  • Unexpectedly, when glucose was removed from the bathing solutions during proximal colon studies, it resulted in a net secretion of Na into the lumen bath instead of absorption.
  • The in vivo studies also demonstrated that VFA absorption was accompanied by an accumulation of HCO3 and a sharp decrease of CO2 in the lumen. This suggested that the hydration of CO2 within the lumen solution or the mucosal cell provided H ions needed to form the more easily permeable undissociated VFA.

Conclusion

  • This research conclude that the absorption of VFAs influenced the rate and direction of net Sodium transport in the large intestine of equines. It could then be explained by the effect of intracellular hydrogen ion concentration on a Sodium-Hydrogen exchange mechanism in one of the opposing cell membranes.

Cite This Article

APA
Argenzio RA, Southworth M, Lowe JE, Stevens CE. (1977). Interrelationship of Na, HCO3, and volatile fatty acid transport by equine large intestine. Am J Physiol, 233(6), E469-E478. https://doi.org/10.1152/ajpendo.1977.233.6.E469

Publication

The American journal of physiology
ISSN: 0002-9513
NlmUniqueID: 0370511
Country: United States
Language: English
Volume: 233
Issue: 6
Pages: E469-E478

Researcher Affiliations

Argenzio, R A
    Southworth, M
      Lowe, J E
        Stevens, C E

          MeSH Terms

          • Acetates / metabolism
          • Acetates / pharmacology
          • Animals
          • Bicarbonates / metabolism
          • Biological Transport / drug effects
          • Biological Transport, Active / drug effects
          • Butyrates / metabolism
          • Chlorides / metabolism
          • Colon / metabolism
          • Fatty Acids / metabolism
          • Female
          • Glucose / pharmacology
          • Horses / metabolism
          • In Vitro Techniques
          • Intestinal Mucosa / metabolism
          • Intestine, Large / metabolism
          • Male
          • Propionates / metabolism
          • Sodium / metabolism

          Citations

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