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Home / Equine Research Bank / Animals (Basel) / Interspecific and Intraspecific Artificial Insemination in D...
Animals : an open access journal from MDPI2023; 13(4); doi: 10.3390/ani13040582

Interspecific and Intraspecific Artificial Insemination in Domestic Equids.

Abstract: Horses and donkeys differ phenotypically and karyotypically, although they can interbreed freely. Eight Standardbred mares and nine Amiata donkey jennies were included in the study. Semen was collected from two horses and two donkey stallions of proven fertility. A first pregnancy diagnosis was performed on day 10 after ovulation and repeated every day until embryo detection or until day 16. Irrespectively of the sire species, pregnancy rates in horse females (20/30, 66.7%) were significantly higher than in donkey females (19/70, 27.1%) (p < 0.05), while horse and donkey males did not affect pregnancy rates. Comparing overall intraspecific and interspecific AI, pregnancy rates were 25/37 (67.6%) and 14/63 (22.2%), respectively (p = 0.0001). The lowest pregnancy rate was obtained when inseminating jennies with horse stallion semen (8/49, 16.3%). No statistical differences were found when comparing embryo diameters, day at first pregnancy diagnosis, or in vitro embryo morphological quality among groups. In this study, much poorer results were obtained with jennies than with mares. Interspecific AI resulted in lower pregnancy rates than intraspecific Al, and AI to produce hinny hybrids resulted in the lowest pregnancy rate. Further studies are required to better understand the mechanism involved in such different outcomes in relation to intra- and interspecific breeding in domestic equids.
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Publication Date: 2023-02-07 PubMed ID: 36830369PubMed Central: PMC9951644DOI: 10.3390/ani13040582Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the effectiveness of interspecific (different species) and intraspecific (same species) artificial insemination in horses and donkeys. It shows that horses generally have a higher pregnancy rate and that interspecies breeding, specially of donkeys with horse semen, results in lower pregnancy rates.

Study Conduct and Analysis

  • The researchers involved eight Standardbred horse mares, nine Amiata donkey jennies, two horse stallions, and two donkey stallions in the study.
  • Semen was collected from the horse and donkey stallions and used for artificial insemination (AI).
  • A pregnancy diagnosis was performed beginning on the 10th day after ovulation and continued daily until an embryo was detected or until the 16th day.

Findings

  • The researchers found that the species of the sire (father) did not significantly affect the pregnancy rates, but the species of the female did.
  • Horse females had significantly higher pregnancy rates (66.7%) compared to donkey females (27.1%).
  • The lowest pregnancy rate was seen when donkey females were inseminated with horse male semen (16.3%).
  • Overall, interspecific AI (between different species) resulted in lower pregnancy rates (22.2%) compared to intraspecific AI (within the same species, 67.6%).
  • The researchers did not find any significant differences in terms of embryo diameters, the day of first pregnancy diagnosis, or the in vitro (in laboratory conditions) embryo morphological quality among groups.

Interpretations and Implications

  • Generally, pregnancy outcomes were poorer with donkeys (jennies) than with horses (mares).
  • The research demonstrates lower success rates for interspecific breeding, particularly when attempting to produce a hinny hybrid (offspring of a male horse and female donkey).
  • The study indicates a need for further research to better understand the mechanisms behind the significantly different outcomes seen in intra- and interspecific breeding in domestic equids (horses and donkeys).

Cite This Article

APA
Fanelli D, Moroni R, Bocci C, Camillo F, Rota A, Panzani D. (2023). Interspecific and Intraspecific Artificial Insemination in Domestic Equids. Animals (Basel), 13(4). https://doi.org/10.3390/ani13040582

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 4

Researcher Affiliations

Fanelli, Diana
  • Veterinary Sciences Department, Pisa University, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
Moroni, Rebecca
  • Veterinary Sciences Department, Pisa University, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
Bocci, Carlotta
  • Veterinary Sciences Department, Pisa University, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
Camillo, Francesco
  • Veterinary Sciences Department, Pisa University, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
Rota, Alessandra
  • Veterinary Sciences Department, Pisa University, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.
Panzani, Duccio
  • Veterinary Sciences Department, Pisa University, Via Livornese, San Piero a Grado, 56124 Pisa, Italy.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 48 references
  1. Gabryś J, Kij B, Kochan J, Bugno-Poniewierska M. Interspecific hybrids of animals-in nature, breeding and science—A review.. Ann. Anim. Sci. 2021;21:403–415.
    doi: 10.2478/aoas-2020-0082google scholar: lookup
  2. Ewing F.F. The Mule as a Factor in the Development of the Southwest.. Ariz. West. 1963;5:315–326.
  3. Essin E.M. Shavetails and Bell Sharps: The History of the US Army Mule.. U of Nebraska Press; Lincoln, NE, USA: 2000.
  4. Arnold W.C. The Mule: The Worker that “Can’t Get No Respect”.. Southwest. Hist. Q. 2008;112:34–50.
    doi: 10.1353/swh.2008.0002google scholar: lookup
  5. Canisso I.F., Panzani D., Miró J., Ellerbrock R.E. Key aspects of donkey and mule reproduction.. Vet. Clin. Equine Pract. 2019;35:607–642.
    doi: 10.1016/j.cveq.2019.08.014pubmed: 31672204google scholar: lookup
  6. Allen W.R., Short R.V. Interspecific and Extraspecific Pregnancies in Equids: Anything Goes.. J. Hered. 1997;88:384–392.
    doi: 10.1093/oxfordjournals.jhered.a023123pubmed: 9378914google scholar: lookup
  7. De Smet J. Horses in wartime: Part 2: Some aspects of the use of horses and other Equidae, mainly mules, in Worldwar I and II.. Vlaams Diergeneeskd. Tijdschr. 2021;90:157–164.
    doi: 10.21825/vdt.v90i3.20417google scholar: lookup
  8. Gray A.P. Mammalian Hybrids: A Check-List with Bibliography.. 2nd ed. Commonwealth Agricultural Bureaux; Wallingford, Oxfordshire, UK: 1972.
  9. Hancock J.L., McGovern P.T. The transport of sheep and goat spermatozoa in the ewe.. Reproduction. 1968;15:283–287.
    doi: 10.1530/jrf.0.0150283pubmed: 5689376google scholar: lookup
  10. Hancock J.L., McGovern P.T., Stamp J.T. Failure of gestation of goat x sheep hybrids in goats and sheep.. J. Reprod. Fertil. Suppl. 1968;3:29–36.
    pubmed: 5288093
  11. Chang M.C., Marston J.H., Hunt D.M. Reciprocal fertilization between the domesticated rabbit and the snowshoe hare with special reference to insemination of rabbits with an equal number of hare and rabbit spermatozoa.. J. Exp. Zool. 1964;155:437–445.
    doi: 10.1002/jez.1401550312pubmed: 14161123google scholar: lookup
  12. Tharasanit T. Artificial Insemination in Horse: A Review.. Board Rev. Ed. 2014;1:113–117.
  13. Samper J.C. Equine Breeding Management and Artificial Insemination.. Elsevier Health Sciences; Riverport Lane; Maryland Heights, MO, USA: 2008.
  14. Morel M.C.D. Equine Reproductive Physiology, Breeding and Stud Management.. CABI; Wallingford, Oxfordshire, England: 2020.
  15. Brinsko S.P., Varner D.D. Artificial insemination and preservation of semen.. Vet. Clin. N. Am. Equine Pract. 1992;8:205–218.
    doi: 10.1016/S0749-0739(17)30476-5pubmed: 1576550google scholar: lookup
  16. De Oliveira J.V., de Luna Freire Oliveira P.V., e Oña C.M.M., Guasti P.N., Monteiro G.A., da Silva Y.F.R.S., Papa P.D.M., Alvarenga M.A., Junior J.A.D., Papa F.O. Strategies to improve the fertility of fresh and frozen donkey semen.. Theriogenology. 2016;85:1267–1273.
    doi: 10.1016/j.theriogenology.2015.12.010pubmed: 26806444google scholar: lookup
  17. Fanelli D., Tesi M., Monaco D., Diaz-Jimenez M., Camillo F., Rota A., Panzani D. Deep-horn Artificial Insemination With Frozen Thawed Semen After re-extension in Autologous Seminal Plasma May Improve Pregnancy Rates in Jennies.. J. Equine Vet. Sci. 2022;112:103932.
    doi: 10.1016/j.jevs.2022.103932pubmed: 35283216google scholar: lookup
  18. Woods J., Bergfelt D.R., Ginther O.J. Effects of time of insemination relative to ovulation on pregnancy rate and embryonic-loss rate in mares.. Equine Vet. J. 1990;22:410–415.
    doi: 10.1111/j.2042-3306.1990.tb04306.xpubmed: 2269264google scholar: lookup
  19. Zeng S., Weigang Y., Shuaishuai W., Jingqian Z., Bing L., Ruitao Z. Technological protocol in reproductive management of intensive raising donkeys; Proceedings of the First International Symposium on Donkey Science, Dong’e; Beijing, China.. 15–17 August 2017; pp. 159–170.
  20. Fanelli D., Tesi M., Rota A., Beltramo M., Camillo F., Panzani D. Studies on the Use of Prostaglandin F2α and Gonadotropin-Releasing Hormone Analogs for Timed Artificial Insemination in Jennies.. J. Equine Vet. Sci. 2018;74:36–41.
    doi: 10.1016/j.jevs.2018.12.001google scholar: lookup
  21. Fanelli D., Tesi M., Bagnato G., Salari F., Martini M., Panzani D., Rota A. New simplified protocols for timed artificial insemination (TAI) in milk-producing donkeys.. Theriogenology. 2019;139:126–131.
    doi: 10.1016/j.theriogenology.2019.08.005pubmed: 31401478google scholar: lookup
  22. Paolucci M., Palombi C., Sylla L., Stradaioli G., Monaci M. Ultrasonographic features of the mule embryo, fetus and fetal-placental unit.. Theriogenology. 2012;77:240–252.
    doi: 10.1016/j.theriogenology.2011.07.030pubmed: 21958639google scholar: lookup
  23. Carluccio A., Bucci R., Fusi J., Robbe D., Veronesi M.C. Effect of age and of reproductive status on reproductive indices in horse mares carrying mule pregnancies.. Heliyon. 2020;6:e05175.
    doi: 10.1016/j.heliyon.2020.e05175pmc: PMC7551364pubmed: 33083622google scholar: lookup
  24. Allen W.R., Kydd J.H., Antczak D.F. Interspecies and extraspecies equine pregnancies.. In: McKlnnon A.O., Voss J.L., editors. Equine Reproduction. Lea and Feblger; Philadelphia, PA, USA: 1993. pp. 536–553.
  25. Carroll C.L., Huntington P.J. Body condition scoring and weight estimation of horses.. Equine Vet. J. 1988;20:41–45.
    doi: 10.1111/j.2042-3306.1988.tb01451.xpubmed: 3366105google scholar: lookup
  26. Cavallini D., Penazzi L., Valle E., Raspa F., Bergero D., Formigoni A., Fusaro I. When changing the hay makes a difference: A series of case reports.. J. Equine Vet. Sci. 2022;113:103940.
    doi: 10.1016/j.jevs.2022.103940pubmed: 35341909google scholar: lookup
  27. Panzani D., Rota A., Tesi M., Fanelli D., Camillo F. Update on donkey embryo transfer and cryopreservation.. J. Equine Vet. Sci. 2018;65:50–54.
    doi: 10.1016/j.jevs.2017.11.012google scholar: lookup
  28. McKinnon A.O., Squires E.L. Equine embryo transfer.. Vet. Clin. N. Am. Equine Pract. 1988;4:305–333.
    doi: 10.1016/S0749-0739(17)30643-0pubmed: 3044542google scholar: lookup
  29. Nishikawa Y. 5th International Congress on Animal Reproduction and Artificial Insemination.. Cornell University; Ithaca, NY, USA: 1964. History and development of artificial insemination in the world; pp. 163–259.
  30. Foote R.H. The history of artificial insemination: Selected notes and notables.. J. Anim. Sci. 2010;80:1–10.
    doi: 10.2527/animalsci2002.80E-Suppl_21agoogle scholar: lookup
  31. Shepherd C. Artificial insemination in mares.. Practice. 2004;26:140–145.
    doi: 10.1136/inpract.26.3.140google scholar: lookup
  32. Raspa F., Cavallarin L., McLean A.K., Bergero D., Valle E. A review of the appropriate nutrition welfare criteria of dairy donkeys: Nutritional requirements, farm management requirements and animal-based indicators.. Animals. 2019;9:315.
    doi: 10.3390/ani9060315pmc: PMC6616590pubmed: 31159459google scholar: lookup
  33. Dezzutto D., Barbero R., Valle E., Giribaldi M., Raspa F., Biasato I., Gennero M.S. Observations of the hematological, hematochemical, and electrophoretic parameters in lactating donkeys (Equus asinus). J. Equine Vet. Sci. 2018;65:1–5.
    doi: 10.1016/j.jevs.2018.02.013google scholar: lookup
  34. Camillo F., Rota A., Biagini L., Tesi M., Fanelli D., Panzani D. The current situation and trend of donkey industry in Europe.. J. Equine Vet. Sci. 2018;66:44–49.
    doi: 10.1016/j.jevs.2017.11.008google scholar: lookup
  35. McKinnon A.O., Squires E.L., Vaala W.E., Varner D.D. Equine Reproduction.. John Wiley & Sons; Hoboken, NJ, USA: 2011.
  36. Trimeche A., Renard P., Tainturier D. A procedure for poitou jackass sperm cryopreservation.. Theriogenology. 1998;50:793–806.
    doi: 10.1016/S0093-691X(98)00184-8pubmed: 10734453google scholar: lookup
  37. Oliveira J.V., Oliveira P.V.L., Melo C.M.M., Guasti P.N., Silva Y.F.R.S., Monteiro G.A. Studies with donkey semen developed at Sao Paulo State University; Proceedings of the First International Symposium on Donkey Science; Dong’e, China.. 15–17 August 2017; Botucatu, Brazil: UNESP; 2017. pp. 142–148.
  38. Rota A., Panzani D., Sabatini C., Camillo F. Donkey jack (Equus asinus) semen cryopreservation: Studies of seminal parameters, post breeding inflammatory response, and fertility in donkey jennies.. Theriogenology. 2012;78:1846–1854.
    doi: 10.1016/j.theriogenology.2012.07.015pubmed: 22979965google scholar: lookup
  39. Miró J., Papas M. Post–Artificial Insemination endometrial inflammation and its control in donkeys.. J. Equine Vet. Sci. 2018;65:38–43.
    doi: 10.1016/j.jevs.2017.11.007google scholar: lookup
  40. Palhares M.S., Silva Filho J.M., Oliveira E.F., Jr. Inseminação artificial em jumentas da raça Pega com cio induzido pelo Dinoprost.. Rev. Bras. Reprod. Anim. 1986;10:157–163.
  41. Silva M.I.F. Viabilidade do uso da inseminação artificial em eqüinos ao nível industrial.. Congresso Brasileiro de Reprodução Animal. Vol. 7. Colégio Brasileiro de Reprodução Animal; Belo Horizonte, Brasil: 1988. pp. 47–63.
  42. Miró J., Marín H., Catalán J., Papas M., Gacem S., Yeste M. Seminal plasma, sperm concentration, and sperm-PMN interaction in the donkey: An in vitro model to study endometrial inflammation at post-insemination.. Int. J. Mol. Sci. 2020;21:3478.
    doi: 10.3390/ijms21103478pmc: PMC7278951pubmed: 32423134google scholar: lookup
  43. Miró J., Vilés K., García W., Jordana J., Yeste M. Effect of donkey seminal plasma on sperm movement and sperm–polymorphonuclear neutrophils attachment in vitro.. Anim. Reprod. Sci. 2013;140:164–172.
    doi: 10.1016/j.anireprosci.2013.06.007pubmed: 23891218google scholar: lookup
  44. Gastal E.L., Santos G.F., Henry M., Piedade H.M. Embryonic and early foetal development in donkeys.. Equine Vet. J. 1993;25:10–13.
    doi: 10.1111/j.2042-3306.1993.tb04815.xgoogle scholar: lookup
  45. Ginther O.J. Ultrasonic Imaging and Animal Reproduction.. Equiservices Pub.; Cross Plains, WI, USA: 1995.
  46. Carluccio A., Villani M., Contri A., Tosi U., Veronesi M.C. Rilievi ecografici della gravidanza precoce nell’ asina di Martina Franca.. Pract. Equina. 2005;16:31–35.
  47. Crisci A., Rota A., Panzani D., Sgorbini M., Ousey J.C., Camillo F. Clinical, ultrasonographic, and endocrinological studies on donkey pregnancy.. Theriogenology. 2014;81:275–283.
    doi: 10.1016/j.theriogenology.2013.09.026pubmed: 24157228google scholar: lookup
  48. Roberts B., Gilbert R., Bergfelt D., Peterson E., Samper J., French H. The early conceptus in Caribbean jennies.. J. Equine Vet. Sci. 2018;66:208–209.
    doi: 10.1016/j.jevs.2018.05.097google scholar: lookup
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