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Home / Equine Research Bank / Virology / Intracellular equine arteritis virus (EAV)-specific RNAs con...
Virology1986; 152(2); 492-496; doi: 10.1016/0042-6822(86)90154-6

Intracellular equine arteritis virus (EAV)-specific RNAs contain common sequences.

Abstract: Equine arteritis virus (EAV) is a nonarthropod-borne togavirus. Six virus-specific RNA species have been found in EAV-infected cells having the following molecular weights: 4.3 X 10(6) (RNA1), 1.3 X 10(6) (RNA2), 0.9 X 10(6) (RNA3), 0.7 X 10(6) (RNA4), 0.3 X 10(6) (RNA5), and 0.2 X 10(6) (RNA6). RNA1 comigrates with the viral genome (M. F. Van Berlo, M. C. Horzinek, and B. A. M. Van der Zeijst, 1982, Virology 118, 345-352). All RNAs hybridized with a radio-labeled cDNA probe representing RNA6, indicating that they contain common sequences. To study this homology in more detail, RNase T1 oligonucleotide fingerprinting of the RNAs was undertaken. This confirmed the presence of common sequences and showed more specifically that the intracellular viral RNAs form a nested set. The number of oligonucleotides in RNA1, however, is only one-third of the expected value. In all aspects studied the replication mechanism of EAV differs from that of other known positive-stranded RNA viruses.
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Publication Date: 1986-07-30 PubMed ID: 3014727DOI: 10.1016/0042-6822(86)90154-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigated the molecular structures of equine arteritis virus (EAV) and found that the virus’s RNA species contain common sequences. Further detail on this commonality was explored using RNase T1 oligonucleotide fingerprinting, revealing a distinct replication mechanism different from other known positive-stranded RNA viruses.

Background and Objectives

  • The research embarks on an in-depth study of the equine arteritis virus (EAV), a nonarthropod-borne togavirus that affects horses.
  • The main aim is to understand more about the molecular structure of EAV, particularly its RNA species, and to study the aspects of its replication mechanism that differ from other known positive-stranded RNA viruses.

Methods and Initial Findings

  • Six different virus-specific RNA species were identified in EAV-infected cells. These RNA species were noted to have varying molecular weights.
  • The researchers also discovered that RNA1 co-migrates with the viral genome, which confirms earlier research done on EAV.
  • All RNAs were found to hybridize with a radio-labeled cDNA probe representing RNA6, indicating they possess common sequences.

Results of RNase T1 Oligonucleotide Fingerprinting

  • To further study the homologous sequences, the researchers utilized RNase T1 oligonucleotide fingerprinting on the RNAs.
  • This method not only confirmed the presence of common sequences previously deduced but also showed that these viral RNAs form a nested set within the infected cells, offering more specificity in the connection between the RNA species.
  • However, the study also observed that the number of oligonucleotides in RNA1 was only one-third of the expected value, an anomaly which warrants further investigation.

Comparison with Other Stranded RNA Viruses

  • The EAV’s replication mechanism was found to be distinctly different from other known positive-stranded RNA viruses.
  • This further emphasizes the need for continued research into EAV’s unique replication mechanism, potentially opening new avenues for treatment or prevention of this equine virus.

Cite This Article

APA
van Berlo MF, Rottier PJ, Horzinek MC, van der Zeijst BA. (1986). Intracellular equine arteritis virus (EAV)-specific RNAs contain common sequences. Virology, 152(2), 492-496. https://doi.org/10.1016/0042-6822(86)90154-6

Publication

Virology
ISSN: 0042-6822
NlmUniqueID: 0110674
Country: United States
Language: English
Volume: 152
Issue: 2
Pages: 492-496

Researcher Affiliations

van Berlo, M F
    Rottier, P J
      Horzinek, M C
        van der Zeijst, B A

          MeSH Terms

          • Animals
          • Base Sequence
          • Cricetinae
          • DNA / analysis
          • Electrophoresis, Agar Gel
          • Equartevirus / genetics
          • Nucleic Acid Hybridization
          • RNA Viruses / genetics
          • RNA, Viral / analysis
          • Ribonuclease T1 / metabolism

          Citations

          This article has been cited 9 times.
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          8. den Boon JA, Snijder EJ, Chirnside ED, de Vries AA, Horzinek MC, Spaan WJ. Equine arteritis virus is not a togavirus but belongs to the coronaviruslike superfamily. J Virol 1991 Jun;65(6):2910-20.
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            doi: 10.1016/0007-1935(92)90044-2pubmed: 1319787google scholar: lookup
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