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Stem cell research & therapy2017; 8(1); 166; doi: 10.1186/s13287-017-0616-0

Isolation and characterization of equine endometrial mesenchymal stromal cells.

Abstract: Equine mesenchymal stromal/stem cells (MSCs) are most commonly harvested from bone marrow (BM) or adipose tissue, requiring the use of surgical procedures. By contrast, the uterus can be accessed nonsurgically, and may provide a more readily available cell source. While human endometrium is known to harbor mesenchymal precursor cells, MSCs have not been identified in equine endometrium. This study reports the isolation, culture, and characterization of MSCs from equine endometrium. The presence of MSC and pericyte markers in endometrial sections was determined using immunohistochemistry. Stromal cells were harvested and cultured after separation of epithelial cells from endometrial fragments using Mucin-1-bound beads. For comparison, MSCs were also harvested from BM. The expression of surface markers in endometrial and BM-derived MSCs was characterized using flow cytometry and quantitative polymerase chain reaction. MSCs were differentiated in vitro into adipogenic, chondrogenic, osteogenic, and smooth muscle lineages. Typical markers of MSCs (CD29, CD44, CD90, and CD105) and pericytes (NG2 and CD146) were localized in the equine endometrium. Both endometrial and BM MSCs grew clonally and robustly expressed MSC and pericyte markers in culture while showing greatly reduced or negligible expression of hematopoietic markers (CD45, CD34) and MHC-II. Additionally, both endometrial and BM MSCs differentiated into adipogenic, osteogenic, and chondrogenic lineages in vitro, and endometrial MSCs had a distinct ability to undergo smooth muscle differentiation. We have demonstrated for the first time the presence of cells in equine endometrium that fulfill the definition of MSCs. The equine endometrium may provide an alternative, easily accessible source of MSCs, not only for therapeutic regeneration of the uterus, but also for other tissues where MSCs from other sources are currently being used therapeutically.
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Publication Date: 2017-07-12 PubMed ID: 28701175PubMed Central: PMC5506676DOI: 10.1186/s13287-017-0616-0Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the presence of mesenchymal stem cells (MSCs) in equine endometrium, comparing them to those from bone marrow, and assesses their differentiation potential. The study concludes that equine endometrium can offer an easily accessible source of MSCs for therapeutic applications.

Isolation and Culture of Equine Endometrial MSCs

  • The investigators used immunohistochemistry to detect the presence of MSCs and pericytes (cells that wrap around the endothelial cells of capillaries and venules) in endometrial sections. They identified usual markers of MSCs and pericytes in the equine endometrium.
  • The endometrial sections were subjected to a process to separate stromal cells (MSCs are found in this category) from the epithelial cells, using Mucin-1-bound beads.
  • The team similarly harvested MSCs from bone marrow to act as a comparison group for their study.

Comparison and Characterization of Endometrial MSCs and Bone Marrow MSCs

  • Researchers used flow cytometry and quantitative polymerase chain reaction to characterize the expression of surface markers in both endometrial and bone marrow-derived MSCs.
  • These MSCs were also differentiated in vitro into adipogenic, osteogenic, and chondrogenic lineages. The research marks a unique ability of endometrial MSCs to differentiate into smooth muscle lineage.
  • Both MSCs from endometrium and bone marrow demonstrated robust growth, rich expression of the MSC markers and pericyte markers in culture. However, they showed minimal to non-existent expression of hematopoietic markers and MHC-II.

Implications of the Findings

  • The study shows for the first time the existence of MSCs in equine endometrium, presenting it as a potential, readily available source of these versatile stem cells.
  • As the uterus can be accessed non-surgically, this source of MSCs eliminates the need for surgical procedures required for retrieving MSCs from bone marrow or adipose tissue.
  • The therapeutic potential of endometrial MSCs extends beyond their application for the regeneration of the uterus to other tissues where MSCs from other sources are presently used therapeutically.

Cite This Article

APA
Rink BE, Amilon KR, Esteves CL, French HM, Watson E, Aurich C, Donadeu FX. (2017). Isolation and characterization of equine endometrial mesenchymal stromal cells. Stem Cell Res Ther, 8(1), 166. https://doi.org/10.1186/s13287-017-0616-0

Publication

Stem cell research & therapy
ISSN: 1757-6512
NlmUniqueID: 101527581
Country: England
Language: English
Volume: 8
Issue: 1
Pages: 166
PII: 166

Researcher Affiliations

Rink, B Elisabeth
  • Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.
  • The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK.
  • University of Veterinary Medicine, 1220, Vienna, Austria.
Amilon, Karin R
  • The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK.
Esteves, Cristina L
  • The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK.
French, Hilari M
  • Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.
Watson, Elaine
  • Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.
Aurich, Christine
  • University of Veterinary Medicine, 1220, Vienna, Austria.
Donadeu, F Xavier
  • The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK. Xavier.Donadeu@roslin.ed.ac.uk.
  • The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK. Xavier.Donadeu@roslin.ed.ac.uk.

MeSH Terms

  • Animals
  • Antigens, Differentiation / metabolism
  • Cell Differentiation
  • Cell Separation / methods
  • Endometrium / cytology
  • Endometrium / metabolism
  • Female
  • Horses
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Muscle, Smooth / cytology
  • Muscle, Smooth / metabolism

Grant Funding

  • Biotechnology and Biological Sciences Research Council

Conflict of Interest Statement

All animal procedures were carried out according to the UK Home Office Animals (Scientific Procedures) Act 1986 with approval by the local Ethical Review Committee. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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