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FEBS letters2002; 531(3); 389-396; doi: 10.1016/s0014-5793(02)03550-0

Isolation of embryonic stem-like cells from equine blastocysts and their differentiation in vitro.

Abstract: Embryonic stem (ES) cells are pluripotent cells with the potential capacity to generate any type of cell. We describe here the isolation of pluripotent ES-like cells from equine blastocysts that have been frozen and thawed. Our two lines of ES-like cells (E-1 and E-2) appear to maintain a normal diploid karyotype indefinitely in culture in vitro and to express markers that are characteristic of ES cells from mice, namely, alkaline phosphatase, stage-specific embryonic antigen-1, STAT-3 and Oct 4. After culture of equine ES-like cells in vitro for more than 17 passages, some ES-like cells differentiated to neural precursor cells in the presence of basic fibroblast growth factor (bFGF), epidermal growth factor and platelet-derived growth factor. We also developed a protocol that resulted in the differentiation of ES-like cells in vitro to hematopoietic and endothelial cell lineages in response to bFGF, stem cell factor and oncostatin M. Our observations set the stage for future developments that may allow the use of equine ES-like cells for the treatment of neurological and hematopoietic disorders.
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Publication Date: 2002-11-19 PubMed ID: 12435581DOI: 10.1016/s0014-5793(02)03550-0Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research describes how stem cells that bear a resemblance to embryonic stem cells can be isolated from horse blastocysts, even after they have been frozen and thawed. These cells can replicate in a lab setting and can differentiate into different types of cells, particularly neural precursor cells and certain types of blood and endothelial cells under specific conditions. This could potentially lead to new approaches for treating neurological and hematopoietic disorders in the future.

Isolation and Maintenance of Embryonic Stem-Like Cells

In the study, the researchers successfully isolated pluripotent embryonic stem-like cells from equine blastocysts, which are early stage embryos. Interestingly, these blastocysts had been previously frozen and thawed. This implies that the blastocysts still retained the vital characteristics that allowed for the harvesting of the pluripotent cells. Key highlights from this section include:

  • The isolated cells were labeled as E-1 and E-2 and demonstrated an ability to sustain a standard diploid karyotype, which refers to a normal chromosomal state, even over an indefinite culture period in a lab setting.
  • These cells also expressed markers characteristic of typical mouse embryonic stem cells such as alkaline phosphatase, stage-specific embryonic antigen-1 (SSEA-1), STAT-3 and Oct 4. These are signifiers of their pluripotent status.

Differentiation of Stem Cells

The differentiation of cells refers to the process in which a cell changes from one type to another. In this context, the researchers observed how the stem cells changed or ‘differentiated’ into other cell types under certain conditions.

  • After culturing the ES-like cells for more than 17 passages, some cells differentiated into neural precursor cells when exposed to basic fibroblast growth factor (bFGF), epidermal growth factor, and platelet-derived growth factor. Neural precursor cells are early-stage cells that have the potential to develop into neurons or other neural cells.
  • A protocol was developed to manipulate the stem cells to differentiate into hematopoietic and endothelial cell lineages. This was done in response to bFGF, stem cell factor and oncostatin M.

Implications for Future Treatment of Disorders

This research establishes a fundamental understanding of how equine stem-like cells can be manipulated in vitro, which could pave the way for future developments in the treatment of neurological and hematopoietic (blood-related) disorders.

  • Development of new therapies or treatments using these stem-like cells could offer hope for conditions that are currently difficult to treat or incurable due to the limitations of the body’s ability to regenerate appropriate cells.
  • Specifically, in horses, this might lead to new treatments for equine neurological disorders or conditions related to their blood circulatory system.

Cite This Article

APA
Saito S, Ugai H, Sawai K, Yamamoto Y, Minamihashi A, Kurosaka K, Kobayashi Y, Murata T, Obata Y, Yokoyama K. (2002). Isolation of embryonic stem-like cells from equine blastocysts and their differentiation in vitro. FEBS Lett, 531(3), 389-396. https://doi.org/10.1016/s0014-5793(02)03550-0

Publication

FEBS letters
ISSN: 0014-5793
NlmUniqueID: 0155157
Country: England
Language: English
Volume: 531
Issue: 3
Pages: 389-396

Researcher Affiliations

Saito, Shigeo
  • Gene Engineering Division, BioResource Center, RIKEN, Tsukuba, 305-0074, Ibaraki, Japan.
Ugai, Hideyo
    Sawai, Ken
      Yamamoto, Yusuke
        Minamihashi, Akira
          Kurosaka, Kahori
            Kobayashi, Yoshiro
              Murata, Takehide
                Obata, Yuichi
                  Yokoyama, Kazunari

                    MeSH Terms

                    • Animals
                    • Base Sequence
                    • Biomarkers
                    • Cell Differentiation
                    • Cell Lineage
                    • DNA Primers
                    • Embryo, Mammalian / cytology
                    • Horses
                    • In Vitro Techniques
                    • Mice
                    • Molecular Sequence Data
                    • Reverse Transcriptase Polymerase Chain Reaction
                    • Stem Cells / cytology

                    Citations

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