Isolation of Y chromosome-specific microsatellites in the horse and cross-species amplification in the genus Equus.
Abstract: Y chromosome polymorphisms such as microsatellites or single nucleotide polymorphisms represent a paternal counterpart to mitochondrial DNA (mtDNA) for evolutionary and phylogeographic studies. The use of Y chromosome haplotyping in natural populations of species other than humans is still hindered by the lack of sequence information necessary for polymorphism screening. Here we used representational difference analysis (RDA) followed by a screen of a bacterial artificial chromosome (BAC) library for repetitive sequences to obtain polymorphic Y-chromosomal markers. The procedure was performed for the domestic horse (Equus caballus) and we report the first six Y-chromosomal microsatellite markers for this species. Three markers were also useful for haplotyping taxa of the zebra/ass lineage. Y-chromosomal microsatellite markers show a single haplotype in the domestic horse, whereas notable variation has been observed in the other members of the genus Equus.
Publication Date: 2004-04-10 PubMed ID: 15073232DOI: 10.1093/jhered/esh020Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research focuses on identifying Y-chromosome polymorphisms, specifically microsatellites, in horses which can be used for evolutionary studies. The researchers developed a procedure using representational difference analysis and other techniques to establish these markers. The study discovered six new Y-chromosomal microsatellite markers in domestic horses and confirmed their limited variation in this species, while finding more variation across other species of the genus Equus.
Research Objective and Procedure
- The aim of the study was to isolate polymorphisms found on the Y-chromosome, specifically microsatellites, in horses. These can be used as a counterpart to mitochondrial DNA for conducting evolutionary and phylogeographic research, similar to studies in humans.
- To achieve their aim, the team of researchers developed a procedure using representational difference analysis (RDA), which is a method used to compare two genomic DNA samples, followed by screening a bacterial artificial chromosome (BAC) library for repetitive sequences.
Key Findings
- The primary outcome of this study was the identification of six new Y-chromosomal microsatellite markers in domestic horses, which is a significant achievement.
- Furthermore, the study found that these markers exhibited a single haplotype in domestic horses, indicating limited variation amongst this species.
- The same procedure was able to identify polymorphisms that were useful for haplotyping taxa of the zebra/ass lineage, highlighting the applicability of this method across different species within the Equus genus.
Implications
- The research provides important tools for investigating paternal lineage in domestic horses and other related species. This holds potential for evolutionary and phylogeographic studies and may lead to valuable insights about the evolution, dispersal, and diversity of these species.
- The study’s finding of notable variance in Y-chromosomal microsatellite markers in members of the genus Equus, apart from the domestic horse, suggests varying evolutionary pathways or rates across species.
Cite This Article
APA
Wallner B, Piumi F, Brem G, Müller M, Achmann R.
(2004).
Isolation of Y chromosome-specific microsatellites in the horse and cross-species amplification in the genus Equus.
J Hered, 95(2), 158-164.
https://doi.org/10.1093/jhered/esh020 Publication
Researcher Affiliations
- Institut für Tierzucht und Genetik, Veterinärmedizinische Universität Wien Veterinärplatz 1, A-1210 Wien, Austria.
MeSH Terms
- Alleles
- Animals
- Base Sequence
- Chromosomes, Artificial, Bacterial
- DNA Primers
- Databases, Genetic
- Electrophoresis, Agar Gel
- Horses / genetics
- Male
- Microsatellite Repeats / genetics
- Molecular Sequence Data
- Polymorphism, Genetic
- Sequence Analysis, DNA
- Y Chromosome / genetics
Citations
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