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Home / Equine Research Bank / Virology / Lentivirus genomic organization: the complete nucleotide seq...
Virology1986; 155(2); 309-321; doi: 10.1016/0042-6822(86)90195-9

Lentivirus genomic organization: the complete nucleotide sequence of the env gene region of equine infectious anemia virus.

Abstract: The nucleotide sequence of the envelope (env) gene region of equine infectious anemia virus (EIAV), a member of the lentivirus subfamily of retroviruses, has been determined from a clone of integrated proviral DNA for which the gag and pol sequences have been reported previously. The env gene is 859 codons in length and the sequence reported here is consistent with the published biochemical properties of EIAV glycoproteins. The env gene region of EIAV shares considerable structural similarities but negligible sequence homologies with the env genes of other members of the lentivirus subfamily, visna virus, and human T-lymphotropic virus (HTLV-III) or lymphadenopathy virus (LAV). As in visna virus and HTLV-III, the polymerase (pol) and env genes of EIAV do not overlap. EIAV contains two short open reading frames (orf) of 50 and 66 codons in the pol-env intergenic region. However, unlike the orf Q regions reported for visna virus and HTLV-III, neither EIAV orf overlaps the 3' terminus of the adjacent pol gene. The EIAV genome also contains a third short open reading frame of 135 codons which is contained completely within the env gene, in contrast to the 3'-orf/orf F gene reported for HTLV-III/LAV which extends beyond the env gene terminus. These results provide a detailed description of the env gene region of EIAV and describe a number of characteristic features of genomic organization in lentiviruses which contrast with the genomic organization of oncogenic retroviruses.
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Publication Date: 1986-12-01 PubMed ID: 2431539DOI: 10.1016/0042-6822(86)90195-9Google Scholar: Lookup
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  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research details the nucleotide sequence of the envelope (env) gene of the equine infectious anemia virus (EIAV). It offers a comparison of EIAV to similar lentiviruses and provides insights into the unique characteristics of lentivirus genomic organization.

Identifying the EIAV env Gene

  • The researchers successfully decoded the nucleotide sequence of the EIAV’s env gene from the DNA of a provirus. A provirus is a virus that’s been integrated into the DNA of a host cell.
  • EIAV’s env gene was found to be 859 codons long. This length and sequence align with previously researched biological characteristics of EIAV’s glycoproteins.

Comparative Analysis of Lentivirus Subfamily

  • The env gene region of EIAV was compared with that of other lentiviruses, namely the visna virus and human T-lymphotropic virus (HTLV-III) or lymphadenopathy virus (LAV).
  • While structural similarities were found between these viruses, sequence homologies were minimal. Homology in this context refers to the similarity in the sequences between different species.
  • The researchers established that the polymerase (pol) and env genes of EIAV, like those in visna virus and HTLV-III, do not overlap.

Genomic Organization in Lentiviruses

  • The EIAV genome contains two short open reading frames (orf) of 50 and 66 codons in the pol-env intergenic region. Interestingly, neither orf overlaps with the 3′ terminus of the adjacent pol gene.
  • A third short orf with 135 codons completely contained within the env gene was also identified in the EIAV genome. This differs from the 3′-orf/orf F gene reported for HTLV-III/LAV, which extends beyond the env gene terminus.
  • The researchers noted that these characteristics of genomic organization are distinctly different from those seen in oncogenic retroviruses, suggesting variant patterns of genomic organization in lentiviruses.

Cite This Article

APA
Rushlow K, Olsen K, Stiegler G, Payne SL, Montelaro RC, Issel CJ. (1986). Lentivirus genomic organization: the complete nucleotide sequence of the env gene region of equine infectious anemia virus. Virology, 155(2), 309-321. https://doi.org/10.1016/0042-6822(86)90195-9

Publication

Virology
ISSN: 0042-6822
NlmUniqueID: 0110674
Country: United States
Language: English
Volume: 155
Issue: 2
Pages: 309-321

Researcher Affiliations

Rushlow, K
    Olsen, K
      Stiegler, G
        Payne, S L
          Montelaro, R C
            Issel, C J

              MeSH Terms

              • Amino Acid Sequence
              • Base Sequence
              • Chromosome Mapping
              • DNA Restriction Enzymes
              • Genes
              • Genes, Viral
              • Infectious Anemia Virus, Equine / genetics
              • Protein Conformation
              • RNA-Directed DNA Polymerase / genetics
              • Repetitive Sequences, Nucleic Acid
              • Solubility
              • Viral Envelope Proteins / genetics

              Grant Funding

              • CA38851 / NCI NIH HHS

              Citations

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