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Virology1989; 172(2); 609-615; doi: 10.1016/0042-6822(89)90203-1

Localization of conserved and variable antigenic domains of equine infectious anemia virus envelope glycoproteins using recombinant env-encoded protein fragments produced in Escherichia coli.

Abstract: Previous characterizations of equine infectious anemia virus (EIAV) glycoprotein variation by DNA sequence analysis and epitope mapping using monoclonal antibodies (MAbs) have revealed the presence of conserved and variable regions within the EIAV env gene. To extend these studies, fragments of the EIAV envelope proteins gp90 and gp45 were expressed in Escherichia coli and used in Western blot analysis with a diverse panel of equine immune sera to identify antigenic segments. All sera from EIAV-infected animals reacted with the carboxyl terminal portion of gp90 and the amino terminal portion of gp45, indicating the highly conserved and immunodominant nature of these regions. Other gp90 segments, both from conserved and variable env sequences, displayed variable reactivities with the panel of equine sera. A panel of MAbs was also used in Western blot assays with the recombinant protein fragments for physical localization of previously identified MAb epitopes. The binding sites of two neutralizing MAbs were localized to a highly variable region of gp90, while nonneutralizing epitopes were localized to conserved and variable regions of the envelope glycoproteins. These results, in addition to localizing important antigenic sites on EIAV glycoproteins, indicate that previously defined conserved and variable env nucleotide sequences indeed encode protein sequences constituting conserved and variable immunogens during persistent infection by EIAV.
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Publication Date: 1989-10-01 PubMed ID: 2552661DOI: 10.1016/0042-6822(89)90203-1Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the identification and localization of the conserved and variable antigenic domains of the Equine Infectious Anemia Virus (EIAV) using fragments of the virus’s envelope proteins. With the help of recombinant proteins produced in Escherichia coli, the researchers were able to determine the reactivity and precise locations of these regions.

Overview of the Study

  • The study aimed to characterize the variations in the EIAV glycoprotein by analyzing its DNA sequence and mapping it using monoclonal antibodies (MAbs) which led them to identify both conserved (unaltered) and variable regions within the EIAV env gene.
  • The research aimed at expanding past studies by expressing fragments of the EIAV envelope proteins gp90 and gp45 within Escherichia coli.
  • These expressed proteins were then used for Western blot analysis in combination with an array of equine immune sera to identify antigenic regions.

Findings of the Study

  • All the sera from EIAV-infected animals reacted to the carboxyl terminal portion of gp90 and the amino terminal portion of gp45, showcasing the consistent and immunodominant nature of these regions.
  • Different gp90 segments, originating from both conserved and variable env sequences, showed varied reactivity with equine sera.
  • The binding sites of two neutralizing monoclonal antibodies were located within a highly variable region of gp90, whereas non-neutralizing epitopes were found in both conserved and variable areas of the envelope glycoproteins.
  • This means that the regions that are defined as conservatively conserved and variable at the genetic level are also correspondingly variable or conserved at the level of immune responses.

Significance of the Research

  • This research is fundamental in pointing out crucial antigenic sites on EIAV glycoproteins, providing a basis for potential future studies on EIAV vaccine development.
  • The finding that the sequences categorized as conserved and variable reflect similar categorization at the protein immunogenic level means that any changes in these areas have direct implications for immunity, vaccination, and disease progression.

Cite This Article

APA
Payne SL, Rushlow K, Dhruva BR, Issel CJ, Montelaro RC. (1989). Localization of conserved and variable antigenic domains of equine infectious anemia virus envelope glycoproteins using recombinant env-encoded protein fragments produced in Escherichia coli. Virology, 172(2), 609-615. https://doi.org/10.1016/0042-6822(89)90203-1

Publication

Virology
ISSN: 0042-6822
NlmUniqueID: 0110674
Country: United States
Language: English
Volume: 172
Issue: 2
Pages: 609-615

Researcher Affiliations

Payne, S L
  • Department of Biochemistry, Louisiana State University, Baton Rouge.
Rushlow, K
    Dhruva, B R
      Issel, C J
        Montelaro, R C

          MeSH Terms

          • Animals
          • Antibodies, Monoclonal / immunology
          • Antigens, Viral / analysis
          • Antigens, Viral / genetics
          • Blotting, Western
          • Cloning, Molecular
          • Electrophoresis, Polyacrylamide Gel
          • Escherichia coli / genetics
          • Glycoproteins / genetics
          • Glycoproteins / immunology
          • Immune Sera / immunology
          • Infectious Anemia Virus, Equine / genetics
          • Infectious Anemia Virus, Equine / immunology
          • Recombinant Fusion Proteins / genetics
          • Recombinant Fusion Proteins / immunology
          • Viral Envelope Proteins / genetics
          • Viral Envelope Proteins / immunology

          Grant Funding

          • AI25850 / NIAID NIH HHS
          • CA38851 / NCI NIH HHS

          Citations

          This article has been cited 22 times.
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