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Home / Equine Research Bank / Theriogenology / Markers of stemness in equine mesenchymal stem cells: a plea...
Theriogenology2010; 75(8); 1431-1443; doi: 10.1016/j.theriogenology.2010.11.008

Markers of stemness in equine mesenchymal stem cells: a plea for uniformity.

Abstract: Mesenchymal stromal cells (MSC) are a very promising subpopulation of adult stem cells for cell-based regenerative therapies in veterinary medicine. Despite major progress in the knowledge on adult stem cells during recent years, a proper identification of MSC remains a challenge. In human medicine, the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy (ISCT) recently proposed three criteria to define MSC. Firstly, cells must be plastic-adherent when maintained under standard culture conditions. Secondly, MSC must express CD73, CD90 and CD105, and lack expression of CD34, CD45, CD14 or CD11b, CD79α or CD19 and MHC class II antigens. Thirdly, MSC must be able to differentiate into osteoblasts, adipocytes and chondroblasts in vitro. Successful isolation and differentiation of equine MSC from different sources such as bone marrow, fat tissue, umbilical cord blood, Wharton's Jelly or peripheral blood has been widely reported. However, their unequivocal immunophenotyping is hampered by the lack of a single specific marker and the limited availability of monoclonal anti-horse antibodies, which are two major factors complicating successful research on equine MSC. Detection of gene expression on mRNA level is hereby a valuable alternative, although the need still exists to test several antibody clones in search for cross-reactivity. To date, commercial antibodies recognizing equine epitopes are only available for CD13, CD44 and MHC-II. Moreover, as the expression of certain adult stem cell markers may differ between species, it is mandatory to define a set of CD markers which can be uniformly applied for the identification of equine MSC.
Copyright © 2011 Elsevier Inc. All rights reserved.
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Publication Date: 2010-12-31 PubMed ID: 21196039DOI: 10.1016/j.theriogenology.2010.11.008Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article talks about the lack of identification method uniformity when it comes to equine mesenchymal stromal cells (MSC), a type of adult stem cells that have significant potential for regenerative therapies in veterinary medicine.

Understanding Mesenchymal Stromal Cells

  • Mesenchymal stromal cells (MSC) are a type of adult stem cells that have a vital role in cell-based regenerative therapies, particularly in veterinary medicine.
  • Despite advances in our understanding of these cells, accurately identifying them remains a challenge.
  • The Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy (ISCT) has recently defined three distinct criteria for defining MSC.

The Three Criteria for Defining MSC

  • The first criterion stipulates that these cells should stick to plastic when preserved under standard culture parameters.
  • The second one specifies that MSC must express specific markers, known as CDs (cluster of differentiation molecules), such as CD73, CD90 and CD105, but should not express CD34, CD45, CD14 or CD11b, CD79α or CD19 and MHC class II antigens.
  • The third requirement points out that MSC should be capable of differentiating into several types of cells, specifically osteoblasts, adipocytes and chondroblasts in a laboratory setting.

Challenges in Equine MSC Research

  • Mesenchymal stromal cells have been efficiently isolated and differentiated from different sources in horses, such as bone marrow, adipose tissue, umbilical cord blood, Wharton’s Jelly or peripheral blood.
  • However, accurate identification of these cells has been complicated by the lack of a specific marker and scarce availability of monoclonal anti-horse antibodies.
  • Due to the lack of appropriate antibodies, researchers resort to detecting gene expression on the mRNA level. Even then, testing must be done to find any possible cross-reactivity between the antibodies used.

Need for a Uniform Identification Method

  • The study highlights the necessity for a standard set of CD markers for the identification of equine MSC as some markers may not express the same way across different species.
  • Currently, commercially available antibodies that recognize equine epitopes are limited to CD13, CD44 and MHC-II.

Cite This Article

APA
De Schauwer C, Meyer E, Van de Walle GR, Van Soom A. (2010). Markers of stemness in equine mesenchymal stem cells: a plea for uniformity. Theriogenology, 75(8), 1431-1443. https://doi.org/10.1016/j.theriogenology.2010.11.008

Publication

Theriogenology
ISSN: 1879-3231
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 75
Issue: 8
Pages: 1431-1443

Researcher Affiliations

De Schauwer, Catharina
  • Department of Reproduction, Obstetrics and Herd Health, Ghent University, Merelbeke, Belgium. Catharina.Deschauwer@UGent.be
Meyer, Evelyne
    Van de Walle, Gerlinde R
      Van Soom, Ann

        MeSH Terms

        • Animals
        • Biomarkers
        • Gene Expression Regulation
        • Horses / physiology
        • Immunophenotyping
        • Mesenchymal Stem Cells / physiology

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