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BMC genetics2013; 14; 83; doi: 10.1186/1471-2156-14-83

Maternal phylogenetic relationships and genetic variation among Arabian horse populations using whole mitochondrial DNA D-loop sequencing.

Abstract: Maternal inheritance is an essential point in Arabian horse population genetics and strains classification. The mitochondrial DNA (mtDNA) sequencing is a highly informative tool to investigate maternal lineages. We sequenced the whole mtDNA D-loop of 251 Arabian horses to study the genetic diversity and phylogenetic relationships of Arabian populations and to examine the traditional strain classification system that depends on maternal family lines using native Arabian horses from the Middle East. Results: The variability in the upstream region of the D-loop revealed additional differences among the haplotypes that had identical sequences in the hypervariable region 1 (HVR1). While the American-Arabians showed relatively low diversity, the Syrian population was the most variable and contained a very rare and old haplogroup. The Middle Eastern horses had major genetic contributions to the Western horses and there was no clear pattern of differentiation among all tested populations. Our results also showed that several individuals from different strains shared a single haplotype, and individuals from a single strain were represented in clearly separated haplogroups. Conclusions: The whole mtDNA D-loop sequence was more powerful for analysis of the maternal genetic diversity in the Arabian horses than using just the HVR1. Native populations from the Middle East, such as Syrians, could be suggested as a hot spot of genetic diversity and may help in understanding the evolution history of the Arabian horse breed. Most importantly, there was no evidence that the Arabian horse breed has clear subdivisions depending on the traditional maternal based strain classification system.
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Publication Date: 2013-09-13 PubMed ID: 24034565PubMed Central: PMC3847362DOI: 10.1186/1471-2156-14-83Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article talks about an extensive study of the genetic diversity and maternal lineage of Arabian horses using mitochondrial DNA (mtDNA) D-loop sequencing. The research uncovers the inefficiency of traditional strain classifications, emphasizing the need for more detailed genetic evaluations for strain differentiation.

Methodology

  • The authors of this article studied the genetic diversity and phylogenetic relationships of Arabian horses using mtDNA D-loop sequencing, a proven method for investigating maternal lineages.
  • The study comprises sequencing the full mtDNA D-loop of 251 Arabian horses, inclusive of native Arabian horses from the Middle East.
  • The article presents a comparative analysis of various Arabian populations, including American-Arabians and the Syrian population.

Results

  • The study revealed major genetic contributions from Middle Eastern horses to Western horses.
  • Results showed some degree of variability in the upstream region of the D-loop among the haplotypes, which demonstrated identical sequences in the hypervariable region 1 (HVR1).
  • The findings determined that the American-Arabian horses demonstrated relatively lower diversity compared to Syrian horses, which were the most variable and comprised a rare and antique haplogroup.
  • Another crucial observation was that there was no evident pattern of differentiation among all the populations studied.
  • The study also showed that various individuals from disparate strains shared a single haplotype, while individuals from a single strain were represented in clearly separated haplogroups.

Conclusions

  • The research concludes that the full mtDNA D-loop sequence was more potent for the analysis of maternal genetic diversity in Arabian horses than just using HVR1.
  • The native Middle-Eastern populations, such as Syrian horses, were proposed as a hot spot of genetic diversity, contributing significantly to understanding the evolution history of Arabian horses.
  • Most importantly, the study found no evidence supporting the traditional strain classification system’s ability to clearly differentiate the Arabian horse breed based on maternal lineage. This highlights the necessity for a more comprehensive genetic evaluation for accurate strain differentiation.

Cite This Article

APA
Khanshour AM, Cothran EG. (2013). Maternal phylogenetic relationships and genetic variation among Arabian horse populations using whole mitochondrial DNA D-loop sequencing. BMC Genet, 14, 83. https://doi.org/10.1186/1471-2156-14-83

Publication

BMC genetics
ISSN: 1471-2156
NlmUniqueID: 100966978
Country: England
Language: English
Volume: 14
Pages: 83

Researcher Affiliations

Khanshour, Anas M
  • Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Science, Texas A&M University, College Station, TX 77843-4458, USA. GCothran@cvm.tamu.edu.
Cothran, Ernest Gus

    MeSH Terms

    • Animals
    • Base Sequence
    • Cluster Analysis
    • DNA, Mitochondrial / genetics
    • Genetic Variation
    • Genetics, Population
    • Haplotypes
    • Horses / classification
    • Horses / genetics
    • Middle East
    • Mitochondria / genetics
    • Phylogeny
    • Principal Component Analysis
    • Sequence Analysis, DNA

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