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The Journal of physiology1990; 431; 173-185; doi: 10.1113/jphysiol.1990.sp018325

Maximum velocity of shortening of three fibre types from horse soleus muscle: implications for scaling with body size.

Abstract: 1. To explore how maximum velocity of shortening (Vmax) of fibres varies within one muscle and how Vmax varies with body size, we measured Vmax of muscle fibres from soleus muscle of a large animal, the horse. 2. Vmax was determined by the slack test on skinned single muscle fibres at 15 degrees C during maximal activation (pCa = 5.2). The fibre type was subsequently determined by a combination of single-cell histochemistry and gel electrophoresis of the myosin light chains. 3. Vmax values for the type I, IIA and IIB muscle fibres were 0.33 +/- 0.04 muscle lengths/s (ML/s) (+/- S.E.M., n = 6), 1.33 +/- 0.08 ML/s (n = 7) and 3.20 +/- 0.26 ML/s (n = 6), respectively. It is likely that the large range in Vmax is due to differences observed in the myosin heavy chains and light chains associated with the three fibre types. 4. Comparison of Vmax over a 1200-fold range (450 kg horse vs. 0.38 kg rat) of body mass (Mb) suggests that slow fibres scale more dramatically (Mb-0.18) than do fast glycolytic fibres (Mb-0.07). This difference may enable the slow fibres to work at high efficiencies in the large animal while the fast fibres can still generate a large mechanical power when necessary.
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Publication Date: 1990-12-01 PubMed ID: 2100306PubMed Central: PMC1181769DOI: 10.1113/jphysiol.1990.sp018325Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research uses the soleus muscle of a horse to study how the maximum velocity of shortening, or Vmax, of muscle fibres differs within a muscle and varies according to body size. The study finds significant variation in Vmax among different types of muscle fibres, likely due to differences in associated myosin chains. Additionally, the study suggests slow muscle fibres scale more dramatically with body size compared to fast glycolytic fibres.

Research Methods

  • The researchers identified the maximum velocity of shortening (Vmax) of muscle fibres from the soleus muscle of a horse, a large animal, to study the differences within one muscle and how Vmax varies with body size.
  • Their method to determine Vmax was the slack test, performed on single muscle fibres that were skinned and undergo maximum activation at 15 degrees Celsius.
  • They further confirmed the fibre type through single-cell histochemistry and gel electrophoresis of the myosin light chains.

Research Findings

  • The study found Vmax values of type I, IIA, and IIB muscle fibres to be 0.33, 1.33, and 3.20 muscle lengths/s respectively, revealing significant variation within one muscle.
  • The researchers attribute the extensive Vmax range to differences in myosin heavy chains and light chains associated with the three muscle fibre types.
  • Comparison of Vmax between a horse and a rat showed that the slow fibres scale more substantially with body mass than the fast glycolytic fibres.
  • The findings suggest that this scaling difference could allow the slow fibres in larger animals to function at high efficiencies while maintaining the ability of the fast fibres to generate significant mechanical power when required.

Cite This Article

APA
Rome LC, Sosnicki AA, Goble DO. (1990). Maximum velocity of shortening of three fibre types from horse soleus muscle: implications for scaling with body size. J Physiol, 431, 173-185. https://doi.org/10.1113/jphysiol.1990.sp018325

Publication

The Journal of physiology
ISSN: 0022-3751
NlmUniqueID: 0266262
Country: England
Language: English
Volume: 431
Pages: 173-185

Researcher Affiliations

Rome, L C
  • Department of Biology, University of Pennsylvania, Leidy Laboratories, Philadelphia 19104.
Sosnicki, A A
    Goble, D O

      MeSH Terms

      • Animals
      • Biomechanical Phenomena
      • Body Constitution / physiology
      • Horses / physiology
      • In Vitro Techniques
      • Isometric Contraction / physiology
      • Muscle Contraction / physiology
      • Muscles / physiology
      • Time Factors

      Grant Funding

      • AR38404 / NIAMS NIH HHS

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