Measurement of 8-hydroxy-2′-deoxyguanosine in serum and cerebrospinal fluid of horses with neuroaxonal degeneration and other causes of proprioceptive ataxia.
Abstract: Eight-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative damage evaluated in human neurodegenerative disease, has potential to correlate with postmortem diagnosis of neuroaxonal dystrophy/degenerative myeloencephalopathy (NAD/DM) in horses. Objective: We hypothesized that 8-OHdG will be higher in CSF and serum from NAD/DM horses compared with horses with other neurologic diseases (CVSM, EPM) and a control group of neurologically normal horses. We also hypothesized that 8-OHdG will be higher in CSF compared with serum from NAD/DM horses. Methods: Fifty client-owned horses with postmortem diagnoses: 20 NAD/DM, 10 CVSM, 10 EPM, and 10 control horses. Serum and CSF samples were obtained between November 2010 and March 2022. Methods: Case-control study using biobanked samples was performed and commercial competitive ELISA kit (Highly Sensitive 8-OHdG Check ELISA) utilized. Concentration of 8-OHdG was quantitated in both CSF and serum and compared between groups. Results: No correlation was established between the measures of 8-OHdG in serum and CSF and group. CSF median [8-OHdG] for NAD/DM was 169.9 pg/mL (IQR : 67.18-210.6), CVSM 157.1 pg/mL (IQR : 132.1-229.1), EPM 131.4 pg/mL (IQR : 102.1-193.2), and control 149.8 pg/mL (IQR : 113.3-196.4). Serum median [8-OHdG] for NAD/DM was 130 pg/mL (IQR : 51.73-157.2), CVSM 125.8 pg/mL (IQR : 62.8-170.8), EPM 120.6 pg/mL (IQR : 87.23-229.7), and control 157.6 pg/mL (IQR : 97.15-245.6). Poisson regression analysis showed no difference established once confounding variables were considered. Conclusions: Eight-OHdG did not aid in antemortem diagnosis of NAD/DM in this cohort of horses. At the time of diagnosis horses with NAD/DM do not have ongoing oxidative stress.
© 2024 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
Publication Date: 2024-01-11 PubMed ID: 38205913PubMed Central: PMC10937501DOI: 10.1111/jvim.16988Google Scholar: Lookup
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Cite This Article
APA
Palmisano M, Kulp J, Bender S, Stefanovski D, Robinson M, Johnson A.
(2024).
Measurement of 8-hydroxy-2′-deoxyguanosine in serum and cerebrospinal fluid of horses with neuroaxonal degeneration and other causes of proprioceptive ataxia.
J Vet Intern Med, 38(2), 1207-1213.
https://doi.org/10.1111/jvim.16988 Publication
Researcher Affiliations
- Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pennsylvania, USA.
- Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pennsylvania, USA.
- Department of Pathobiology, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pennsylvania, USA.
- Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pennsylvania, USA.
- Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pennsylvania, USA.
- Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pennsylvania, USA.
MeSH Terms
- Humans
- Animals
- Horses
- 8-Hydroxy-2'-Deoxyguanosine
- Neurodegenerative Diseases / veterinary
- Case-Control Studies
- NAD
- Horse Diseases / diagnosis
- Neuroaxonal Dystrophies / veterinary
- Ataxia / veterinary
Grant Funding
- 1-400666-xxxx-2000-5919 / Firestone/Tamworth/Raker-Tulleners Grant
Conflict of Interest Statement
Authors declare no conflict of interest.
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Citations
This article has been cited 1 times.- Șerban M, Toader C, Covache-Busuioc RA. The Redox Revolution in Brain Medicine: Targeting Oxidative Stress with AI, Multi-Omics and Mitochondrial Therapies for the Precision Eradication of Neurodegeneration.. Int J Mol Sci 2025 Aug 3;26(15).
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