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Home / Equine Research Bank / J Equine Sci / Measuring V̇O2 in hypoxic and hyperoxic conditions usi...
Journal of equine science2019; 30(4); 87-92; doi: 10.1294/jes.30.87

Measuring V̇O2 in hypoxic and hyperoxic conditions using dynamic gas mixing with a flow-through indirect calorimeter.

Abstract: Measurements of gas exchange while breathing gases of different O concentrations are useful in respiratory and exercise physiology. High bias flows required in flow-through indirect calorimetry systems for large animals like exercising horses necessitate the use of inconveniently large reservoirs of mixed gases for making such measurements and can limit the amount of equilibration time that is adequate for steady-state measurements. We obviated the need to use a pre-mixed reservoir of gas in a semi-open flow-through indirect calorimeter by dynamically mixing gases and verified the theoretical accuracy and utility of making such measurements using the mass-balance N-dilution method. We evaluated the accuracy of the technique at different inspired oxygen fractions by measuring exercising oxygen consumption (O) at two fully aerobic submaximal exercise intensities in Thoroughbred horses. Horses exercised at 24% and 50% maximum oxygen consumption (O max) of each horse while breathing different O concentrations (19.5%, 21% and 25% O). The N-dilution technique was used to calculate O. Repeated-measures ANOVA was used to tested for differences in O between different inspired O concentrations. The specific O of the horses trotting at 24%Omax and cantering at 50%Omax were not significantly different among the three different inspired oxygen fractions. These findings demonstrate that reliable measurements of O can be obtained at various inspired oxygen fractions using dynamic gas mixing and the N-dilution technique to calibrate semi-open-circuit gas flow systems.
©2019 The Japanese Society of Equine Science.
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Publication Date: 2019-12-18 PubMed ID: 31871410PubMed Central: PMC6920056DOI: 10.1294/jes.30.87Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study discusses the successful implementation of dynamic gas mixing in a flow-through indirect calorimeter to measure oxygen consumption (V̇O2) in horses exercising in different oxygen environments. This technique removes the need for pre-mixed gas reservoirs and proves to provide reliable measurements.

Objective of the Research

  • The primary goal of this research was to circumvent the requirement of using large reservoirs of pre-mixed gases for measuring gas exchange in energetic large animals, like horses, by dynamically mixing gases in a semi-open flow-through indirect calorimeter.

Theoretical Framework and Method

  • The researchers employed the mass-balance nitrogen-dilution method to verify the theoretical accuracy and practicality of their measurements.
  • The idea was to evaluate the precision of the technique across various inspired oxygen fractions by measuring the oxygen consumption (V̇O2) in Thoroughbred horses performing exercise at two fully aerobic submaximal intensities.
  • Experiments were conducted while horses exercised at 24% and 50% of their maximum oxygen consumption levels, breathing in different oxygen concentrations (19.5%, 21%, and 25%).
  • To calculate V̇O2, the nitrogen-dilution technique was employed.
  • A repeated-measures ANOVA was performed to identify differences in V̇O2 between the different inspired oxygen concentrations.

Results and Findings

  • There was no significant difference in the specific V̇O2 of the horses trotting at 24% of their max V̇O2 and cantering at 50% max V̇O2 across the three different inspired oxygen fractions.
  • Therefore, these findings suggest that reliable measurements of V̇O2 can be acquired at various inspired oxygen fractions using dynamic gas mixing and the nitrogen-dilution method to calibrate semi-open-circuit gas flow systems.

Cite This Article

APA
Birks EK, Ohmura H, Jones JH. (2019). Measuring V̇O2 in hypoxic and hyperoxic conditions using dynamic gas mixing with a flow-through indirect calorimeter. J Equine Sci, 30(4), 87-92. https://doi.org/10.1294/jes.30.87

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 30
Issue: 4
Pages: 87-92

Researcher Affiliations

Birks, Eric K
  • Equine Sports Medicine Consultants, DE 19711, U.S.A.
Ohmura, Hajime
  • Sports Science Division, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
Jones, James H
  • Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA 95616, U.S.A.

References

This article includes 26 references
  1. Birks EK, Jones JH, Berry JD. Plasma lactate kinetics in exercising horses. 1991. pp. 179–187. In: Equine Exercise Physiology 3 (Persson, S.G.B., Lindholm, A. and Jeffcott, L.B. eds.), ICEEP Publications, Uppsala.
  2. Cain SM. Gas exchange in hypoxia, apnea, and hyperoxia. 1987. pp. 403–420. In: Handbook of Physiology (Fishman, A.P. ed.), American Physiological Society, Bethesda.
  3. Cissik JH, Johnson RE, Rokosch DK. Production of gaseous nitrogen in human steady-state conditions.. J Appl Physiol 1972 Feb;32(2):155-9.
    pubmed: 5007862doi: 10.1152/jappl.1972.32.2.155google scholar: lookup
  4. Davies RE, Noe FE, Whitty AJ, Busby MG, Davies KR. Breath-by-breath measurement of oxygen consumption and FIO2-FEO2 with increased oxygen demand.. Acta Anaesthesiol Scand 1991 Apr;35(3):201-4.
    pubmed: 2038925doi: 10.1111/j.1399-6576.1991.tb03273.xgoogle scholar: lookup
  5. Dudka LT, Inglis HJ, Johnson RE, Pechinski JM, Plowman S. Inequality of inspired and expired gaseous nitrogen in man.. Nature 1971 Jul 23;232(5308):265-8.
    pubmed: 4937081doi: 10.1038/232265b0google scholar: lookup
  6. Evans DL, Rose RJ. Cardiovascular and respiratory responses in Thoroughbred horses during treadmill exercise.. J Exp Biol 1988 Jan;134:397-408.
    pubmed: 3356965doi: 10.1242/jeb.134.1.397google scholar: lookup
  7. Fedak MA, Rome L, Seeherman HJ. One-step N2-dilution technique for calibrating open-circuit VO2 measuring systems.. J Appl Physiol Respir Environ Exerc Physiol 1981 Sep;51(3):772-6.
    pubmed: 7327980doi: 10.1152/jappl.1981.51.3.772google scholar: lookup
  8. Gillespie JR, Landgren GL, Leith DE. 1:2 ratio of breathing to stride frequencies in a galloping horse breathing 6% CO2. 1991. pp. 66–70. In: Equine Exercise Physiology 3 (Persson, S.G.B., Lindholm, A. and Jeffcott, L.B. eds.), ICEEP Publications, Uppsala.
  9. Jones JH. Optimization of the mammalian respiratory system: symmorphosis versus single species adaptation.. Comp Biochem Physiol B Biochem Mol Biol 1998 May;120(1):125-38.
    pubmed: 9787782doi: 10.1016/s0305-0491(98)00027-3google scholar: lookup
  10. Jones JH, Birks EK, Berry JD. Does oxygen transport limit VO2max in horses?. FASEB J 1988. 3: 430.
  11. Jones JH, Carlson GP. Estimation of metabolic energy cost and heat production during a 3-day-event.. Equine Vet J Suppl 1995 Nov;(20):23-30.
    pubmed: 8933081doi: 10.1111/j.2042-3306.1995.tb05004.xgoogle scholar: lookup
  12. Jones JH, Longworth KE, Lindholm A, Conley KE, Karas RH, Kayar SR, Taylor CR. Oxygen transport during exercise in large mammals. I. Adaptive variation in oxygen demand.. J Appl Physiol (1985) 1989 Aug;67(2):862-70.
    pubmed: 2793686doi: 10.1152/jappl.1989.67.2.862google scholar: lookup
  13. Jones JH, Ohmura H, Stanley SD, Hiraga A. Energetic cost of locomotion on different equine treadmills.. Equine Vet J Suppl 2006 Aug;(36):365-9.
    pubmed: 17402449doi: 10.1111/j.2042-3306.2006.tb05570.xgoogle scholar: lookup
  14. Karas RH, Taylor CR, Jones JH, Lindstedt SL, Reeves RB, Weibel ER. Adaptive variation in the mammalian respiratory system in relation to energetic demand: Vii. Flow of oxygen across the pulmonary gas exchanger. Respir. Physiol. 1987. 69: 101–115.
  15. Landgren GL, Gillespie JR, Leith DE. No ventilatory response to CO2 in Thoroughbreds galloping at 14 m/s. 1991. pp. 59–65. In: Equine Exercise Physiology 3 (Persson, S.G.B., Lindholm, A., and Jeffcott, L.B. eds.), ICEEP Publications, Uppsala.
  16. Ohmura H, Hiraga A, Jones JH. Method for quantifying net anaerobic power in exercising horses.. Equine Vet J Suppl 2006 Aug;(36):370-3.
    pubmed: 17402450doi: 10.1111/j.2042-3306.2006.tb05571.xgoogle scholar: lookup
  17. Ohmura H, Mukai K, Takahashi T, Matsui A, Hiraga A, Jones JH. Comparison of net anaerobic energy utilisation estimated by plasma lactate accumulation rate and accumulated oxygen deficit in Thoroughbred horses.. Equine Vet J Suppl 2010 Nov;(38):62-9.
    pubmed: 21058984doi: 10.1111/j.2042-3306.2010.00261.xgoogle scholar: lookup
  18. Pascoe JR, Hiraga A, Hobo S, Birks EK, Yarbrough TB, Takahashi T, Hada T, Aida H, Steffey EP, Jones JH. Cardiac output measurements using sonomicrometer crystals on the left ventricle at rest and exercise.. Equine Vet J Suppl 1999 Jul;(30):148-52.
    pubmed: 10659240doi: 10.1111/j.2042-3306.1999.tb05206.xgoogle scholar: lookup
  19. Pelletier N, Leith DE. Cardiac output but not high pulmonary artery pressure varies with FIO2 in exercising horses.. Respir Physiol 1993 Jan;91(1):83-97.
    pubmed: 8441873doi: 10.1016/0034-5687(93)90091-ngoogle scholar: lookup
  20. Pelletier N, Leith DE. Ventilation and carbon dioxide exchange in exercising horses: effect of inspired oxygen fraction.. J Appl Physiol (1985) 1995 Feb;78(2):654-62.
    pubmed: 7759436doi: 10.1152/jappl.1995.78.2.654google scholar: lookup
  21. Seeherman HJ, Taylor CR, Maloiy GM, Armstrong RB. Design of the mammalian respiratory system. II. Measuring maximum aerobic capacity.. Respir Physiol 1981 Apr;44(1):11-23.
    pubmed: 7232881doi: 10.1016/0034-5687(81)90074-8google scholar: lookup
  22. Stanek KA, Nagle FJ, Bisgard GE, Byrnes WC. Effect of hyperoxia on oxygen consumption in exercising ponies.. J Appl Physiol Respir Environ Exerc Physiol 1979 Jun;46(6):1115-8.
    pubmed: 468633doi: 10.1152/jappl.1979.46.6.1115google scholar: lookup
  23. Taylor CR, Karas RH, Weibel ER, Hoppeler H. Adaptive variation in the mammalian respiratory system in relation to energetic demand.. Respir Physiol 1987 Jul;69(1):1-127.
    pubmed: 3616184doi: 10.1016/0034-5687(87)90097-1google scholar: lookup
  24. Wagner PD, Erickson BK, Seaman J, Kubo K, Hiraga A, Kai M, Yamaya Y. Effects of altered FIO2 on maximum VO2 in the horse.. Respir Physiol 1996 Aug;105(1-2):123-34.
    pubmed: 8897658doi: 10.1016/0034-5687(96)00044-8google scholar: lookup
  25. Welch HG, Mullin JP, Wilson GD, Lewis J. Effects of breathing O2-enriched gas mixtures on metabolic rate during exercise.. Med Sci Sports 1974 Spring;6(1):26-32.
    pubmed: 4826688
  26. Welch HG, Pedersen PK. Measurement of metabolic rate in hyperoxia.. J Appl Physiol Respir Environ Exerc Physiol 1981 Sep;51(3):725-31.
    pubmed: 7327974doi: 10.1152/jappl.1981.51.3.725google scholar: lookup

Citations

This article has been cited 2 times.
  1. Ebisuda Y, Mukai K, Takahashi Y, Yoshida T, Kawano A, Matsuhashi T, Miyata H, Kuwahara M, Ohmura H. Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle.. Front Vet Sci 2023;10:1230212.
    doi: 10.3389/fvets.2023.1230212pubmed: 37671280google scholar: lookup
  2. Ohmura H, Mukai K, Takahashi Y, Takahashi T. Metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue.. Sci Rep 2021 May 27;11(1):11261.
    doi: 10.1038/s41598-021-90834-ypubmed: 34045613google scholar: lookup
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