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Biology of reproduction2000; 62(5); 1402-1408; doi: 10.1095/biolreprod62.5.1402

Meiotic competence in horse oocytes: interactions among chromatin configuration, follicle size, cumulus morphology, and season.

Abstract: Horse oocytes were collected from an abattoir over a 15-mo period. After classification of follicle size and cumulus morphology, oocytes were either fixed immediately (0 h) or matured in vitro (24 h). There was no effect of season on the number of antral follicles present on the ovaries, or on oocyte maturation rate for any class of oocyte. The proportion of oocytes having condensed chromatin at 0 h increased with increasing follicle size. The oocyte maturation rate also increased with follicle size, and for follicles </= 20-mm diameter, was higher for oocytes initially having expanded cumuli than for those having compact cumuli. The maturation rate was strongly correlated (r(2) = 0.92) with the proportion of oocytes having condensed chromatin at 0 h. Oocytes with diffuse chromatin were found essentially only in follicles </= 20-mm diameter that yielded compact granulosa, indicating follicle viability. Presence of diffuse chromatin was inversely related to maturation rate. We conclude that the major signal for chromatin condensation, and thus acquisition of meiotic competence, occurs in viable follicles after 20-mm diameter in the horse. Condensation of chromatin in oocytes in smaller apparently viable follicles, while associated with acquisition of meiotic competence, may represent a pre-atretic change.
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Publication Date: 2000-04-25 PubMed ID: 10775193DOI: 10.1095/biolreprod62.5.1402Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research is about how chromatin configuration, follicle size, cumulus morphology, and season affect the meiotic capability of horse oocytes, which was studied over a 15-month period. The study found a firm correlation between the oocyte maturation rate and the proportion of oocytes with condensed chromatin.

Methodology

  • The researchers collected horse oocytes from an abattoir over a period of 15 months.
  • After classification according to follicle size and cumulus morphology, the oocytes were either fixed immediately (0 h) or matured in an in vitro environment for a 24-hour period.
  • The researchers then studied the effects of different variables such as season, follicle size, and cumulus morphology on the number of antral follicles present on the ovaries, the rate of oocyte maturation, and the chromatin configuration within the oocytes.

Key Findings

  • Season had no apparent influence on the number of antral follicles present on the ovaries or the rate of oocyte maturation for any category of oocyte.
  • The proportion of oocytes having condensed chromatin at 0 h increased with the size of the follicle.
  • The maturation rate of the oocytes also increased with the follicle size. For follicles with a diameter of less than or equal to 20 mm, the maturation rate was higher for oocytes having expanded cumuli than those with compact cumuli.
  • Oocytes with diffuse chromatin were essentially found only in follicles with a diameter of less than or equal to 20 mm that yielded compact granulosa. This indicates the viability of the follicle.

Conclusions

  • The research concluded that the major signal for chromatin condensation, and thus the acquisition of meiotic competence, occurs in viable follicles after they reach a diameter of 20 mm in horses.
  • While associated with the acquisition of meiotic competence, condensation of chromatin in oocytes in smaller viable follicles could represent a pre-atretic (follicle degenerating) change.

Cite This Article

APA
Hinrichs K, Schmidt AL. (2000). Meiotic competence in horse oocytes: interactions among chromatin configuration, follicle size, cumulus morphology, and season. Biol Reprod, 62(5), 1402-1408. https://doi.org/10.1095/biolreprod62.5.1402

Publication

Biology of reproduction
ISSN: 0006-3363
NlmUniqueID: 0207224
Country: United States
Language: English
Volume: 62
Issue: 5
Pages: 1402-1408

Researcher Affiliations

Hinrichs, K
  • Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA. khinrichs@cvm.tamu.edu
Schmidt, A L

    MeSH Terms

    • Animals
    • Cell Size
    • Chromatin / ultrastructure
    • Female
    • Horses
    • Meiosis
    • Oocytes / cytology
    • Ovarian Follicle / cytology
    • Seasons

    Citations

    This article has been cited 15 times.
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