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Veterinary journal (London, England : 1997)2014; 202(2); 361-366; doi: 10.1016/j.tvjl.2014.08.021

Membrane vesicles mediate pro-angiogenic activity of equine adipose-derived mesenchymal stromal cells.

Abstract: Multipotent mesenchymal stromal cells (MSCs) have attracted a great deal of interest, due to several distinctive features, including the ability to migrate to damaged tissue and to participate in tissue regeneration. There is increasing evidence that membrane vesicles (MVs), comprising exosomes and shedding vesicles, represent a key component, responsible for many of the paracrine effects of MSCs. The aim of the present study was to establish whether equine adipose-derived MSCs (E-AdMSCs) produce MVs that are capable of influencing angiogenesis, a key step in tissue regeneration. A morphological study was performed using MSC monolayers, prepared for transmission and scanning electron microscopy and on ultracentrifuged MSC supernatants, to identify production of MVs. The ability of MVs to influence angiogenesis was evaluated by means of the rat aortic ring and scratch assays. The results demonstrated that MVs, constitutively produced by E-AdMSCs, are involved in intercellular communication with endothelial cells, stimulating angiogenesis. Although many questions remain regarding their formation, delivery, content and mechanism of action, the present study supports the concept that MVs released by MSCs have the potential to be exploited as a therapeutic tool for regenerative medicine.
Copyright © 2014 Elsevier Ltd. All rights reserved.
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Publication Date: 2014-08-26 PubMed ID: 25241947DOI: 10.1016/j.tvjl.2014.08.021Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the production of membrane vesicles (MVs) by equine adipose-derived mesenchymal stromal cells (E-AdMSCs) and their potential role in stimulating angiogenesis, a critical process in tissue regeneration. The results highlight that these MVs could be a potential therapeutic tool in regenerative medicine.

Adipose-Derived Mesenchymal Stromal Cells

  • The research focuses on Multipotent mesenchymal stromal cells (MSCs) derived from equine adipose tissue (E-AdMSCs).
  • These cells have the capabilities to migrate to damaged tissue and participate in tissue regeneration, making them a point of interest in the field of regenerative medicine.

Membrane Vesicles and Their Role

  • MVs, which include exosomes and shedding vesicles, are found to be a crucial element responsible for the paracrine effects of MSCs. These effects involve communication and influence between different cell types.
  • The primary goal of this research was to determine whether E-AdMSCs produce MVs that are able to impact angiogenesis, an essential step in tissue regeneration. Angiogenesis involves the formation of new blood vessels in the body.

Morphological Study and Evaluation of MV Influence

  • A morphological study was carried out using MSC monolayers, a single layer of cells. These were developed for transmission and scanning electron microscopy, for identifying the production of MVs.
  • MVs’ ability to influence angiogenesis was assessed through the rat aortic ring and scratch assays. These assays are experimental models for studying angiogenesis.

Results and Implications

  • The results indicated that MVs, which are constitutively produced by E-AdMSCs, are involved in intercellular communication with endothelial cells, stimulating angiogenesis.
  • While there are still many questions regarding their formation, delivery, content, and mechanism of action, the study supports the idea that MVs released by MSCs show promise to be exploited as a therapeutic tool for regenerative medicine.

Cite This Article

APA
Pascucci L, Alessandri G, Dall'Aglio C, Mercati F, Coliolo P, Bazzucchi C, Dante S, Petrini S, Curina G, Ceccarelli P. (2014). Membrane vesicles mediate pro-angiogenic activity of equine adipose-derived mesenchymal stromal cells. Vet J, 202(2), 361-366. https://doi.org/10.1016/j.tvjl.2014.08.021

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 202
Issue: 2
Pages: 361-366
PII: S1090-0233(14)00342-6

Researcher Affiliations

Pascucci, Luisa
  • Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy. Electronic address: luisa.pascucci@unipg.it.
Alessandri, Giulio
  • IRCCS Foundation, Neurological Institute 'C. Besta', Cerebrovascular Diseases Unit, Via Celoria 11, 20133 Milan, Italy.
Dall'Aglio, Cecilia
  • Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
Mercati, Francesca
  • Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
Coliolo, Paola
  • Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
Bazzucchi, Cinzia
  • Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
Dante, Sara
  • Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
Petrini, Stefano
  • Experimental Zooprophylactic Institute of Umbria and Marche, Immunology Unit, Via G.Salvemini 1, 06126 Perugia, Italy.
Curina, Giovanni
  • Experimental Zooprophylactic Institute of Umbria and Marche, Immunology Unit, Via G.Salvemini 1, 06126 Perugia, Italy.
Ceccarelli, Piero
  • Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.

MeSH Terms

  • Adipose Tissue / physiology
  • Angiogenesis Inducing Agents
  • Animals
  • Cell Membrane Structures / physiology
  • Horses / physiology
  • Mesenchymal Stem Cell Transplantation / veterinary
  • Mesenchymal Stem Cells / physiology
  • Neovascularization, Physiologic

Citations

This article has been cited 28 times.
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