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American journal of veterinary research2000; 61(11); 1451-1455; doi: 10.2460/ajvr.2000.61.1451

Methicillin-resistant coagulase-negative staphylococci isolated from healthy horses in Japan.

Abstract: To determine patterns of methicillin-resistant staphylococci isolated from apparently healthy horses. Methods: 44 horses from 8 riding clubs in Japan. Methods: Methicill in-resistant staphylococci were isolated from the skin or nares, using a selective medium containing a beta-(symboric) lactam antibiotic, ceftizoxime. Clonality of isolates was determined by use of pulsed-field gel electrophoresis. Detection of mecA, mecl, and mecR1 genes was accomplished by use of polymerase chain reactions. Results: Of the 44 horses, 13 (29.5%) yielded 15 isolates of methicillin-resistant staphylococci. The 15 isolates were identified as 6 species (Staphylococcus epidermidis, S lentus, S saprophyticus, S xylosus, S sciuri, and S haemolyticus). However, methicillin-resistant S aureus was seldom isolated. Each isolate contained the mecA gene and had a high resistance to beta-lactam antibiotics. Some isolates also were resistant to other antibiotics such as erythromycin and kanamycin. Conclusions: Methicillin-resistant coagulase-negative staphylococci that were highly resistant to various antibiotics were isolated from apparently healthy horses in Japan. These organisms must be considered a potential threat to horses and veterinarians who care for them.
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Publication Date: 2000-12-07 PubMed ID: 11108196DOI: 10.2460/ajvr.2000.61.1451Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the prevalence and types of methicillin-resistant bacteria, specifically coagulase-negative staphylococci, in healthy horses in Japan. It found that such resistant strains that could potentially threaten the health of horses and their caregivers are present in the equine population.

Research Methodology

  • The study was carried out on 44 horses from 8 different riding clubs in Japan.
  • Methicillin-resistant staphylococci were isolated from the skin or nares (the nasal passage) of these horses using a specific medium containing the antibiotic ceftizoxime, which is a beta-lactam antibiotic.
  • Pulsed-field gel electrophoresis, a laboratory technique used for the separation and identification of large DNA molecules, was used to determine the clonality or genetic similarity of the isolated bacteria.
  • Specific genes linked with antibiotic resistance (mecA, mecl, and mecR1) in the bacteria were identified using polymerase chain reaction (PCR), a common method to amplify and detect DNA sequences.

Research Findings

  • Out of the 44 horses sampled, 13 horses (or 29.5% of the total) yielded 15 isolates of methicillin-resistant staphylococci.
  • The bacteria isolated belonged to six species (Staphylococcus epidermidis, S lentus, S saprophyticus, S xylosus, S sciuri, and S haemolyticus). Interestingly, methicillin-resistant S aureus, a common and often problematic human pathogen, was seldom isolated.
  • Each of the bacterial isolates contained the resistance gene mecA and demonstrated high resistance to beta-lactam antibiotics.
  • Additional resistance to other antibiotics such as erythromycin and kanamycin was observed in some isolates.

Conclusion

  • The study concludes that methicillin-resistant coagulase-negative staphylococci, highly resistant to various antibiotics, are present in apparently healthy horses in Japan.
  • This finding suggests these organisms could pose a potential health risk to horses and those who care for them, including veterinarians, and underline the need for careful healthcare practices and possible additional control measures.

Cite This Article

APA
Yasuda R, Kawano J, Onda H, Takagi M, Shimizu A, Anzai T. (2000). Methicillin-resistant coagulase-negative staphylococci isolated from healthy horses in Japan. Am J Vet Res, 61(11), 1451-1455. https://doi.org/10.2460/ajvr.2000.61.1451

Publication

American journal of veterinary research
ISSN: 0002-9645
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 61
Issue: 11
Pages: 1451-1455

Researcher Affiliations

Yasuda, R
  • Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Japan.
Kawano, J
    Onda, H
      Takagi, M
        Shimizu, A
          Anzai, T

            MeSH Terms

            • Animals
            • Bacterial Proteins / genetics
            • Bacteriological Techniques / methods
            • Bacteriological Techniques / veterinary
            • Carrier Proteins / genetics
            • Coagulase / analysis
            • DNA Primers
            • Electrophoresis, Agar Gel / veterinary
            • Electrophoresis, Gel, Pulsed-Field / veterinary
            • Hexosyltransferases
            • Horses / microbiology
            • Japan
            • Methicillin Resistance
            • Microbial Sensitivity Tests / veterinary
            • Muramoylpentapeptide Carboxypeptidase / genetics
            • Penicillin-Binding Proteins
            • Peptidyl Transferases
            • Polymerase Chain Reaction / veterinary
            • Repressor Proteins / genetics
            • Skin / microbiology
            • Staphylococcus / drug effects
            • Staphylococcus / enzymology
            • Staphylococcus / isolation & purification

            Citations

            This article has been cited 12 times.
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