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Stem cell research & therapy2015; 6(1); 66; doi: 10.1186/s13287-015-0037-x

Microencapsulated equine mesenchymal stromal cells promote cutaneous wound healing in vitro.

Abstract: The prevalence of impaired cutaneous wound healing is high and treatment is difficult and often ineffective, leading to negative social and economic impacts for our society. Innovative treatments to improve cutaneous wound healing by promoting complete tissue regeneration are therefore urgently needed. Mesenchymal stromal cells (MSCs) have been reported to provide paracrine signals that promote wound healing, but (i) how they exert their effects on target cells is unclear and (ii) a suitable delivery system to supply these MSC-derived secreted factors in a controlled and safe way is unavailable. The present study was designed to provide answers to these questions by using the horse as a translational model. Specifically, we aimed to (i) evaluate the in vitro effects of equine MSC-derived conditioned medium (CM), containing all factors secreted by MSCs, on equine dermal fibroblasts, a cell type critical for successful wound healing, and (ii) explore the potential of microencapsulated equine MSCs to deliver CM to wounded cells in vitro. Methods: MSCs were isolated from the peripheral blood of healthy horses. Equine dermal fibroblasts from the NBL-6 (horse dermal fibroblast cell) line were wounded in vitro, and cell migration and expression levels of genes involved in wound healing were evaluated after treatment with MSC-CM or NBL-6-CM. These assays were repeated by using the CM collected from MSCs encapsulated in core-shell hydrogel microcapsules. Results: Our salient findings were that equine MSC-derived CM stimulated the migration of equine dermal fibroblasts and increased their expression level of genes that positively contribute to wound healing. In addition, we found that equine MSCs packaged in core-shell hydrogel microcapsules had similar effects on equine dermal fibroblast migration and gene expression, indicating that microencapsulation of MSCs does not interfere with the release of bioactive factors. Conclusions: Our results demonstrate that the use of CM from MSCs might be a promising new therapy for impaired cutaneous wounds and that encapsulation may be a suitable way to effectively deliver CM to wounded cells in vivo.
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Publication Date: 2015-04-11 PubMed ID: 25889766PubMed Central: PMC4413990DOI: 10.1186/s13287-015-0037-xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research is about how microencapsulated mesenchymal stromal cells (MSCs) derived from horses can promote wound healing, examining the effects of microencapsulated MSCs on equine dermal fibroblasts, and exploring an effective delivery system for these cells.

Understanding the Research

  • The study is based on the high prevalence of impaired cutaneous (skin) wound healing. Current treatments are often ineffective and can lead to negative social and economic impacts.
  • The existing hole in our knowledge regarding wound healing are two-fold; first, how mesenchymal stromal cells (MSCs) exert their effects on the cells they target is not fully understood. Secondly, there is no known effective and safe delivery system to supply these MSC-derived factors to their target cells.
  • This research aims to better understand the role of MSCs in wound healing. It uses equine MSC-derived conditioned medium (CM), a nutrient-rich liquid in which cells have been fragmented and contains all the factors secreted by MSCs, to study how these cells influence equine dermal fibroblasts, which play a crucial role in wound healing.
  • The researchers also explore microencapsulation, a potential method for delivering MSCs to wound cells. Mesenchymal stromal cells are encapsulated in small, biologically inert particles, sort of medical ‘microcapsules’.

Methods and Findings

  • The researchers extracted MSCs from the peripheral blood of healthy horses. They wounded equine dermal fibroblasts from the NBL-6 (horse dermal fibroblast cell) line in vitro and evaluated their migration and gene expression levels after treatment with MSC-derived conditioned medium or NBL-6 derived conditioned medium.
  • Post-treatment, they observed the effects of the conditioned medium collected from microencapsulated MSCs on the wounded fibroblasts.
  • Results marked a considerable increase in fibroblast migration and gene expression linked with wound healing after the treatment with MSC-derived conditioned medium. They also found that encapsulated MSCs had similar effects, indicating that the process did not hinder the release of bioactive factors.

Conclusions

  • The study concludes that the conditioned medium derived from MSCs can potentially offer an effective new therapy for impaired skin wounds.
  • Additionally, encapsulation could serve as an efficient method to deliver this medium to the wounded cells in vivo, enabling a more targeted therapeutic approach.

Cite This Article

APA
Bussche L, Harman RM, Syracuse BA, Plante EL, Lu YC, Curtis TM, Ma M, Van de Walle GR. (2015). Microencapsulated equine mesenchymal stromal cells promote cutaneous wound healing in vitro. Stem Cell Res Ther, 6(1), 66. https://doi.org/10.1186/s13287-015-0037-x

Publication

Stem cell research & therapy
ISSN: 1757-6512
NlmUniqueID: 101527581
Country: England
Language: English
Volume: 6
Issue: 1
Pages: 66

Researcher Affiliations

Bussche, Leen
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 235 Hungerford Hill Road, Ithaca, NY, 14850, USA. lb548@cornell.edu.
Harman, Rebecca M
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 235 Hungerford Hill Road, Ithaca, NY, 14850, USA. rmh12@cornell.edu.
Syracuse, Bethany A
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 235 Hungerford Hill Road, Ithaca, NY, 14850, USA. bas346@cornell.edu.
Plante, Eric L
  • Department of Biological Sciences, State University of New York at Cortland, 21 Graham Avenue, Cortland, NY, 13045, USA. eric.plante@cortland.edu.
Lu, Yen-Chun
  • Department of Biological and Environmental Engineering, Cornell University, Wing Road, Ithaca, NY, 14850, USA. yl2347@cornell.edu.
Curtis, Theresa M
  • Department of Biological Sciences, State University of New York at Cortland, 21 Graham Avenue, Cortland, NY, 13045, USA. theresa.curtis@cortland.edu.
Ma, Minglin
  • Department of Biological and Environmental Engineering, Cornell University, Wing Road, Ithaca, NY, 14850, USA. mm826@cornell.edu.
Van de Walle, Gerlinde R
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 235 Hungerford Hill Road, Ithaca, NY, 14850, USA. grv23@cornell.edu.

MeSH Terms

  • Animals
  • Cell Line
  • Cell Movement / drug effects
  • Cell Proliferation
  • Cell- and Tissue-Based Therapy / methods
  • Chemokine CXCL10 / biosynthesis
  • Cobalt / pharmacology
  • Culture Media, Conditioned / pharmacology
  • Female
  • Fibroblasts / metabolism
  • Gene Expression / drug effects
  • Guided Tissue Regeneration / methods
  • Horses
  • Interferon-gamma / pharmacology
  • Interleukin-8 / biosynthesis
  • Matrix Metalloproteinase 1 / biosynthesis
  • Matrix Metalloproteinase 13 / biosynthesis
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stem Cells / physiology
  • Mitomycin / pharmacology
  • Models, Animal
  • Skin / injuries
  • Skin Diseases / therapy
  • Tumor Necrosis Factor-alpha / pharmacology
  • Wound Healing / drug effects
  • Wound Healing / physiology

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