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Home / Equine Research Bank / Biomed Res Int / Molecular Detection of Equine Herpesvirus Types 1 and 4 Infe...
BioMed research international2015; 2015; 917854; doi: 10.1155/2015/917854

Molecular Detection of Equine Herpesvirus Types 1 and 4 Infection in Healthy Horses in Isfahan Central and Shahrekord Southwest Regions, Iran.

Abstract: This study was undertaken to investigate molecularly the occurrence of EHV-1 and EHV-4 infection among equine population in regions, Iran. Blood samples from 53 and 37 randomly selected horses settled in Isfahan and Shahrekord, Iran, respectively, were collected. Detection of EHV-1 and EHV-4 genes in the blood samples was done using polymerase chain reaction (PCR). Out of 53 and 37 samples from Isfahan and Shahrekord, 4 (18.18%) and 3 (8.10%) were positive for PCR of EHV-1, respectively. Nine (16.98%) and 6 (16.21%) were positive for PCR of EHV-4, while 6 (11.32%) and 3 (8.10%) were positive for PCR of both EHV-1 and EHV-4, in Isfahan and Shahrekord, respectively. Of the 7 blood samples positive for EHV-1, 4 (16.66%) and 3 (8.10%) were from horses >3 years old while 2 (18.18%) and 1 (16.66%) were from 2-3 years old horses, in Isfahan and Shahrekord, respectively. Out of the 7 and 3 samples positive for PCR of EHV-1 in Isfahan and Shahrekord, 4 (22.2%) and 1 (7.69%) were Standardbred, while 3 (14.28%) and 2 (13.33%) were Thoroughbreds, respectively. EHV-4 was detected in blood of 4 (22.22%) and 2 (15.83%) Standardbreds and from 4 (19.04%) and 4 (26.66%) Thoroughbred horses in Isfahan and Shahrekord, respectively. This study has shown that horses settled in Isfahan central and Shahrekord southwest regions, Iran, are infected by EHV-1 and EHV-4 and thus serve as potential reservoirs and disseminators of the viruses.
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Publication Date: 2015-09-01 PubMed ID: 26421307PubMed Central: PMC4569764DOI: 10.1155/2015/917854Google Scholar: Lookup
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  • Journal Article

Summary

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The research explores the presence of Equine Herpesvirus types 1 and 4 in healthy horses in specific regions of Iran using molecular detection techniques.

Objective and Methodology

  • The study aims to understand the occurrence of Equine Herpesvirus types 1 and 4 (EHV-1 and EHV-4) infections among horses in Isfahan central and Shahrekord southwest regions of Iran. The selected areas are presumably significant regarding the equine population.
  • Researchers collected blood samples from randomly picked horses, 53 from Isfahan and 37 from Shahrekord.
  • The detection of EHV-1 and EHV-4 genes in the blood samples was achieved by employing the Polymerase Chain Reaction (PCR) technique. PCR is a widely-used method in molecular biology to amplify and thus detect even small amounts of specific DNA segments.

Results

  • In the collected samples, the results demonstrated the presence of EHV-1 in 18.18% and 8.10% of samples from Isfahan and Shahrekord, respectively. Additionally, EHV-4 was detected in 16.98% and 16.21% of samples from Isfahan and Shahrekord, correspondingly.
  • Interestingly, the incidences where both EHV-1 and EHV-4 were found simultaneously were 11.32% and 8.10% in Isfahan and Shahrekord, respectively.
  • The study also identified infection rates based on the age of the horses. Horses older than 3 years had higher EHV-1 infection rates than younger horses across both regions.
  • Finally, the study presented the EHV-1 and EHV-4 infection rates by horse breeds. Both Standardbred and Thoroughbred varieties showed signs of infection.

Conclusion

  • The study concludes that horses located in the Isfahan central and Shahrekord southwest regions of Iran demonstrate infections of EHV-1 and EHV-4 even while seeming healthy.
  • As a result, these horses serve as potential reservoirs and disseminators of the viruses. High infection rates, coupled with the asymptomatic nature of the condition in some cases, may lead to extensive virus propagation without detection.

Cite This Article

APA
Taktaz Hafshejani T, Nekoei S, Vazirian B, Doosti A, Khamesipour F, Anyanwu MU. (2015). Molecular Detection of Equine Herpesvirus Types 1 and 4 Infection in Healthy Horses in Isfahan Central and Shahrekord Southwest Regions, Iran. Biomed Res Int, 2015, 917854. https://doi.org/10.1155/2015/917854

Publication

BioMed research international
ISSN: 2314-6141
NlmUniqueID: 101600173
Country: United States
Language: English
Volume: 2015
Pages: 917854
PII: 917854

Researcher Affiliations

Taktaz Hafshejani, Taghi
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Nekoei, Shahin
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Vazirian, Behnam
  • Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Doosti, Abbas
  • Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Khamesipour, Faham
  • Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Anyanwu, Madubuike Umunna
  • Microbiology Unit, Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.

MeSH Terms

  • Aging
  • Animals
  • Breeding
  • Electrophoresis, Agar Gel
  • Genes, Viral
  • Herpesviridae Infections / veterinary
  • Herpesviridae Infections / virology
  • Herpesvirus 1, Equid / genetics
  • Herpesvirus 4, Equid / genetics
  • Horse Diseases / virology
  • Horses / virology
  • Iran

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Citations

This article has been cited 4 times.
  1. Barbosa JD, Lins AMC, Bomjardim HDA, Silveira NDSES, Barbosa CC, Beuttemmuller EA, Brito MF, Salvarani FM. Equine Herpesvirus Type 1 Myeloencephalitis in the Brazilian Amazon.. Animals (Basel) 2022 Dec 23;13(1).
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  2. Wilcox A, Barnum S, Wademan C, Corbin R, Escobar E, Hodzic E, Schumacher S, Pusterla N. Frequency of Detection of Respiratory Pathogens in Clinically Healthy Show Horses Following a Multi-County Outbreak of Equine Herpesvirus-1 Myeloencephalopathy in California.. Pathogens 2022 Oct 8;11(10).
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  3. Khamesipour F, Taktaz-Hafshejani T, Tebit KE, Razavi SM, Hosseini SR. Prevalence of endo- and ecto-parasites of equines in Iran: A systematic review.. Vet Med Sci 2021 Jan;7(1):25-34.
    doi: 10.1002/vms3.321pubmed: 32644306google scholar: lookup
  4. Hue ES, Richard EA, Fortier CI, Fortier GD, Paillot R, Raue R, Pronost SL. Equine PBMC Cytokines Profile after In Vitro α- and γ-EHV Infection: Efficacy of a Parapoxvirus Ovis Based-Immunomodulator Treatment.. Vaccines (Basel) 2017 Sep 19;5(3).
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