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Molecular biology reports2013; 40(10); 5861-5867; doi: 10.1007/s11033-013-2692-2

Molecular phylogeny of Indian horse breeds with special reference to Manipuri pony based on mitochondrial D-loop.

Abstract: Manipuri pony is the geographically distant breed of horse from the five recognized horse breeds found in the Indian subcontinent. The phylogenetic relationship of Manipuri pony with the other breeds is unknown. The diversity in the mitochondrial (mt) DNA D-loop region is employed as an important tool to understand the origin and genetic diversification of domestic horses and to examine genetic relationships among breeds around the world. This study was carried out to understand the maternal lineages of Manipuri pony using the 247 bp region of the mtDNA D-loop. The dataset comprised of eleven numbers of self developed sequences of Manipuri pony, 59 and 35 number of retrieved sequences of Indian horse breeds and other worldwide breeds respectively. A total of 35 haplotypes was identified with a high level of genetic diversity in the Indian breeds. A total of seven major mtDNA haplogroups (A-G) was identified in the Indian horse breeds that indicated the abundance of mtDNA diversity and multiple origins of maternal lineages in them. The majority of the studied sequences of Indian breeds (33.3 %) were grouped into haplogroup D and least (3.9 %) in haplogroup E. The Manipuri breed showed the least FST distance (0.03866) with the most diverged Indian breeds and with Thoroughbred horse among the worldwide. This study indicated a close association between Manipuri pony and Thoroughbred.
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Publication Date: 2013-09-26 PubMed ID: 24068432DOI: 10.1007/s11033-013-2692-2Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article examines the genetic relationship of the Manipuri pony, an Indian horse breed, with other recognized breeds using mitochondrial DNA. The study identified a high level of genetic diversity in Indian breeds and multiple origins of maternal lineages. Importantly, the findings indicate a close association between the Manipuri pony and the world-renowned Thoroughbred breed.

Research Objective and Methodology

  • The intent of the research was to understand the maternal lineage of the Manipuri pony, a breed found in India that is geographically distant from five other recognized horse breeds in the region. Unlike other breeds, the phylogenetic relationship of the Manipuri pony had not been thoroughly explored.
  • The researchers made use of the mitochondrial (mt) DNA D-loop region. This region provides crucial data on the origin, genetic diversification, and genetic relationship among different breeds around the globe.
  • A dataset consisting of self-developed sequences from the Manipuri pony, pre-existing sequences from other Indian horse breeds, and also sequences from global horse breeds was employed. The sequences covered a 247 base pair region in the mtDNA D-loop.

Research Findings

  • The research identified 35 distinct haplotypes among the sampled horse breeds, indicating a high degree of genetic diversity among Indian horse breeds. A haplotype refers to a grouping of specific alleles (gene variations) in an organism that are inherited together.
  • A total of seven major mtDNA haplogroups, labelled A to G, were discovered in Indian horse breeds. Haplogroups reflect groupings of similar haplotypes that share a common ancestor and, thus, enhance our understanding of genetic kinship and diversification.
  • The majority of sequences from Indian horse breeds (33.3%) were categorized under haplogroup D, while the smallest proportion (3.9%) was filed under haplogroup E.
  • The data suggested that the Manipuri pony had the least genetic distance (as indicated by an FST measure of 0.03866) from the most diverged Indian breeds and the Thoroughbred breed, a globally recognized horse breed. Essentially, this implies a close association between the Manipuri pony and the Thoroughbred at a genetic level.

Significance of the Research

  • The findings underscore the rich genetic diversity of Indian horse breeds and the multiple origins of maternal lineages within this population.
  • The research provides a deeper understanding of the genetic links between different horse breeds in India, adding valuable information to the fields of genetic diversity and molecular phylogeny studies.
  • Perhaps most significantly, the study reveals a close genetic association between the Manipuri pony and the Thoroughbred breed. This connection expands our understanding of global horse genetics and could impact future breeding strategies.

Cite This Article

APA
Devi KM, Ghosh SK. (2013). Molecular phylogeny of Indian horse breeds with special reference to Manipuri pony based on mitochondrial D-loop. Mol Biol Rep, 40(10), 5861-5867. https://doi.org/10.1007/s11033-013-2692-2

Publication

Molecular biology reports
ISSN: 1573-4978
NlmUniqueID: 0403234
Country: Netherlands
Language: English
Volume: 40
Issue: 10
Pages: 5861-5867

Researcher Affiliations

Devi, Kshetrimayum Miranda
  • Department of Biotechnology, Assam University, Silchar, 788011, Assam, India.
Ghosh, Sankar Kumar

    MeSH Terms

    • Animals
    • Base Sequence
    • Breeding
    • DNA, Mitochondrial / chemistry
    • DNA, Mitochondrial / genetics
    • Genetic Variation
    • Haplotypes / genetics
    • Horses / genetics
    • India
    • Molecular Sequence Data
    • Nucleic Acid Conformation
    • Phylogeny

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    Citations

    This article has been cited 6 times.
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