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Home / Equine Research Bank / Vet Parasitol / Molecular studies on Babesia, Theileria and Hepatozoon in so...
Veterinary parasitology2003; 113(3-4); 189-201; doi: 10.1016/s0304-4017(03)00078-5

Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe. Part I. Epizootiological aspects.

Abstract: Molecular epizootiology of piroplasmids (Babesia spp., Theileria spp.) and Hepatozoon canis was studied in mammals from southern Europe (mainly from Spain, but also from Portugal and France). Partial amplification and sequencing of the 18s rRNA gene was used for molecular diagnosis. In some particular cases (B. ovis and B. bovis) the complete 18s rRNA gene was sequenced. Blood samples were taken from domestic animals showing clinical symptoms: 10 dogs, 10 horses, 10 cows, 9 sheep and 1 goat. In addition, DNA samples were isolated from blood of 12 healthy dogs and from spleen of 10 wild red foxes (Vulpes vulpes). The results of the survey were the following: Piroplasmid infections: Approximately from 50 to 70% of wild or domestic mammals (symptomatic) were infected. Piroplasmids detected in ruminants were:COW: B. bovis, T. annulata and Theileria sp. (type C). Sheep and goat: B. ovis. Piroplasmids present in canids were: Babesia canis vogeli, Babesia canis canis, Theileria annae and B. equi. The only piroplasmid found in asymptomatic dogs was B. equi. Piroplasmids found in horse were: B. equi and B. canis canis.H. canis infections in canids: H. canis was absent of domestic dog samples, whereas all foxes studied were infected by this protozoa. Genetic analysis showed that most of piroplasmid and Hepatozoon isolates from southern Europe matched unambigously with previously described species, as demonstrated by the high level sequence identity between them, usually between 99 and 100%. Minor differences, usually detected in hypervariable regions of 18s rRNA gene are probably due to strain variations or rare genetic polymorphisms. A possible exception was B. bovis, which shows a relatively lower degree of homology (94%) with regard to other B. bovis isolates from several countries. The same is true for B. ovis, that showed a 94% identity with regard to Babesia sp. from South African cow and a 92% with rapport to B. bovis from Portugal.
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Publication Date: 2003-04-30 PubMed ID: 12719133DOI: 10.1016/s0304-4017(03)00078-5Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study conducted molecular tests on three species of protozoan parasites, Babesia, Theileria, and Hepatozoon, in mammals from Southern Europe to explore their patterns of infection, variations in their genetic structure, and to identify the parasites found in different mammalian hosts.

Methodology

  • The study was conducted primarily in Spain, along with certain areas of Portugal and France.
  • Partial amplification and sequencing of the 18s rRNA gene was used to diagnose the infection.
  • In some cases, the complete 18s rRNA gene of B. ovis and B. bovis was sequenced.
  • Blood samples were taken from clinically symptomatic domestic animals – 10 each from dogs, horses, and cows; 9 from sheeps; and 1 from a goat. Additionally, asymptomatic DNA samples were isolated from the blood of 12 healthy dogs and the spleen of 10 wild red foxes.

Results

  • About 50-70% of wild or domestic mammals were found to be infected with Piroplasmid infections.
  • Different species of Piroplasmids were detected in different animals – cows showed presence of B. bovis, T. annulata, and Theileria sp. (type C), while sheeps and goat were infected with B. ovis. Canids were found to have Babesia canis vogeli, Babesia canis canis, Theileria annae, and B. equi.
  • The only piroplasmid found in asymptomatic dogs was B. equi and horses showed presence of B. equi and B. canis canis.
  • Domestic dogs were free from H. canis infections while all examined foxes were infected by this protozoa.
  • Genetic analysis showed that most of the isolated piroplasmids and Hepatozoon from southern Europe aligned unambiguously with previously described species with high sequence identity, typically between 99 and 100%.
  • Minor differences found in hypervariable regions of the 18s rRNA gene are likely due to strain variations or rare genetic polymorphisms.
  • B. bovis and B. ovis exhibited a relatively lower degree of homology (94%) with other similar isolates from many countries.

Conclusion

  • The study gives a deep insight into the epizootiological aspects of these three parasitic infections in southern Europe, providing useful information for designing preventative and treatment strategies.
  • It also highlighted the high genetic similarity between the isolates from southern Europe with those of previously described species, with only minor variances likely due to strain differences or rare genetic polymorphisms.

Cite This Article

APA
Criado-Fornelio A, Martinez-Marcos A, Buling-Saraña A, Barba-Carretero JC. (2003). Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe. Part I. Epizootiological aspects. Vet Parasitol, 113(3-4), 189-201. https://doi.org/10.1016/s0304-4017(03)00078-5

Publication

Veterinary parasitology
ISSN: 0304-4017
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 113
Issue: 3-4
Pages: 189-201

Researcher Affiliations

Criado-Fornelio, A
  • Laboratory of Parasitology, Microbiology and Parasitology Department, Faculty of Pharmacy, University of Alcalá de Henares, 28871, Alcalá de Henares, Spain. angel.criado@uah.es
Martinez-Marcos, A
    Buling-Saraña, A
      Barba-Carretero, J C

        MeSH Terms

        • Animal Diseases / epidemiology
        • Animal Diseases / parasitology
        • Animals
        • Babesia / genetics
        • Babesia / isolation & purification
        • Babesiosis / epidemiology
        • Babesiosis / parasitology
        • Babesiosis / veterinary
        • Base Sequence
        • Cattle
        • Coccidia / genetics
        • Coccidia / isolation & purification
        • Coccidiosis / epidemiology
        • Coccidiosis / parasitology
        • Coccidiosis / veterinary
        • DNA, Protozoan / chemistry
        • DNA, Protozoan / genetics
        • Dogs
        • Foxes
        • France / epidemiology
        • Goats
        • Horses
        • Molecular Epidemiology
        • Molecular Sequence Data
        • Polymerase Chain Reaction / veterinary
        • Portugal
        • RNA, Ribosomal, 18S / chemistry
        • RNA, Ribosomal, 18S / genetics
        • Sheep
        • Spain / epidemiology
        • Theileria / genetics
        • Theileria / isolation & purification
        • Theileriasis / epidemiology
        • Theileriasis / parasitology

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