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Molecular Typing of Burkholderia mallei Isolates from Equids with Glanders, India.
Abstract: We collected 10 Burkholderia mallei isolates from equids in 9 districts in India during glanders outbreaks in 2013-2016. Multilocus variable-number tandem-repeat analysis showed 7 outbreak area-related genotypes. The study highlights the utility of this analysis for epidemiologically tracing of specific B. mallei isolates during outbreaks.
Publication Date: 2021-05-21 PubMed ID: 34013856PubMed Central: PMC8153868DOI: 10.3201/eid2706.203232Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study revolves around the genetic investigation of the Burkholderia mallei bacterium, responsible for causing glanders in horses, using samples from 9 districts in India between 2013 and 2016. It emphasizes the use of multilocus variable-number tandem-repeat analysis to trace specific strains of the bacteria during outbreaks.
Overview of the Research Process
- The research started with the collection of 10 different strains of the Burkholderia mallei bacterial pathogen. These were collected from infected horses in 9 diverse districts in India during outbreaks between 2013 and 2016.
- The focus of the study was the bacterium Burkholderia mallei, which is the causative agent of glanders, a highly infectious and lethal disease affecting horses and other equids.
Use of Multilocus Variable-number Tandem-repeat Analysis
- Key to this study was the use of a technique known as multilocus variable-number tandem-repeat (VNTR) analysis. This is a method used in molecular biology to categorize and differentiate strains of bacteria based on their genetic profiles.
- This form of genetic analysis aids in the identification of the genetic diversity within the collected bacterial samples. By using VNTR analysis, the research team was able to identify seven different genotypes related to the outbreak areas.
- This process provides a relatively high degree of genetic resolution, meaning that it can provide a detailed individuation of different bacterial strains. This makes it particularly useful in tracking and tracing the path of specific bacterial isolates during an outbreak.
Significance of the Study
- The importance of this study lies in its demonstration of the practical utility of VNTR analysis in tracing specific B. mallei strains during outbreaks.
- This form of molecular typing has the potential to improve our understanding of disease transmission and therefore improve measures to control and prevent the spread of glanders in equid populations.
- This research also adds to the body of knowledge regarding B. mallei genotypes in India, which can assist future studies in understanding the genetic diversity and epidemiology of this bacterium in this region.
Cite This Article
APA
Singha H, Elschner MC, Malik P, Saini S, Tripathi BN, Mertens-Scholz K, Brangsch H, Melzer F, Singh RK, Neubauer H.
(2021).
Molecular Typing of Burkholderia mallei Isolates from Equids with Glanders, India.
Emerg Infect Dis, 27(6), 1745-1748.
https://doi.org/10.3201/eid2706.203232 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Burkholderia mallei / genetics
- Glanders
- Horses
- India
- Minisatellite Repeats
- Molecular Typing
References
This article includes 10 references
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- Malik P, Singha H, Khurana SK, Kumar R, Kumar S, Raut AA. Emergence and re-emergence of glanders in India: a description of outbreaks from 2006 to 2011. Vet Ital 2012;48:167–78.
- Malik P, Singha H, Goyal SK, Khurana SK, Tripathi BN, Dutt A. Incidence of Burkholderia mallei infection among indigenous equines in India. Vet Rec Open 2015;2:e000129.
- Singha H, Shanmugasundaram K, Tripathi BN, Saini S, Khurana SK, Kanani A. Serological surveillance and clinical investigation of glanders among indigenous equines in India from 2015 to 2018. Transbound Emerg Dis 2020;67:1336–48.
- Godoy D, Randle G, Simpson AJ, Aanensen DM, Pitt TL, Kinoshita R. Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol 2003;41:2068–79.
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- Hornstra H, Pearson T, Georgia S, Liguori A, Dale J, Price E. Molecular epidemiology of glanders, Pakistan. Emerg Infect Dis 2009;15:2036–9.
- Girault G, Wattiau P, Saqib M, Martin B, Vorimore F, Singha H. High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei. Infect Genet Evol 2018;63:1–4.
- Wernery U, Wernery R, Joseph M, Al-Salloom F, Johnson B, Kinne J. Natural Burkholderia mallei infection in Dromedary, Bahrain. Emerg Infect Dis 2011;17:1277–9.
- Scholz HC, Pearson T, Hornstra H, Projahn M, Terzioglu R, Wernery R. Genotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events. PLoS Negl Trop Dis 2014;8:e3195.
Citations
This article has been cited 3 times.- Grause JF, Elschner MC, Ledesma NA, Murphy G. Development and validation of a chemiluminescent western blot assay for glanders (Burkholderia mallei) serodetection. J Vet Diagn Invest 2024 Mar;36(2):283-286.
- Brangsch H, Singha H, Laroucau K, Elschner M. Sequence-based detection and typing procedures for Burkholderia mallei: Assessment and prospects. Front Vet Sci 2022;9:1056996.
- Shanmugasundaram K, Singha H, Saini S, Tripathi BN. 16S rDNA and ITS Sequence Diversity of Burkholderia mallei Isolated from Glanders-Affected Horses and Mules in India (2013-2019). Curr Microbiol 2021 Dec 18;79(1):31.
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