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Home / Equine Research Bank / Equine Vet J / Monitoring the fate of autologous and allogeneic mesenchymal...
Equine veterinary journal2008; 40(2); 178-181; doi: 10.2746/042516408X276942

Monitoring the fate of autologous and allogeneic mesenchymal progenitor cells injected into the superficial digital flexor tendon of horses: preliminary study.

Abstract: Autologous mesenchymal progenitor cells (MPCs) purified from bone marrow aspirates are being used in the treatment of superficial digital flexor tendon (SDFT) injuries in the horse with promising results. In this study the fate of autologous and allogeneic MPCs following injection into the SDFT was monitored by stable transfection of MPCs with green fluorescent protein (GFP). Small lesions were created manually in one forelimb SDFT of 2 horses and injected with autologous MPCs, allogeneic MPCs or bone marrow supernatant alone. Post mortem examinations performed after 10 or 34 days revealed GFP labelled cells located mainly within injected lesions, but with a small proportion integrated into the crimp pattern of adjacent healthy areas of tendon. Furthermore, there was no visible cell mediated immune response to allogeneic MPCs in either of the host horses.
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Publication Date: 2008-02-13 PubMed ID: 18267891DOI: 10.2746/042516408X276942Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the results of injecting purified bone marrow cells into tendon injuries in horses. It utilizes a green fluorescent protein to track the whereabouts of these cells once injected. The study notes promising outcomes, including integration of these cells into healthy areas of the tendon.

Study Overview

  • The research’s main objective was to investigate the use of autologous and allogeneic mesenchymal progenitor cells (MPCs) in the treatment of superficial digital flexor tendon (SDFT) injuries in horses.
  • The researchers opted to track the cells by marking them with green fluorescent protein (GFP), allowing them to observe whether and where the cells integrated into the animals’ tissues.

Methodology

  • The team first isolated the mesenchymal progenitor cells from bone marrow aspirates. These are essentially immature cells that can potentially develop into multiple types of tissue.
  • These cells were then injected into the SDFT of two horses in which small lesions had been manually created.
  • In the experiment, one horse was administered autologous cells (cells from its own body) while the other received allogeneic cells (cells from another horse).
  • For controls, bone marrow supernatant alone, devoid of these cells, was also used.

Findings

  • After the horses were euthanized, post mortem examinations revealed that the GFP-labelled cells were mostly located within the lesions where they had been injected. However, a small number of cells were found to have integrated into healthy area of the tendon suggesting possible repair activity.
  • Interestingly, the study did not observe any visible cell-mediated immune response in either of the horses who received allogeneic cells. This suggests that cells from different horses (allogeneic) did not trigger an immune reaction, marking the potential for use of such cells in therapeutic settings.

Implication of Results

  • These promising results indicate that MPCs, whether autologous or allogeneic, could potentially be used in treating tendon injuries in horses.
  • The lack of a visible immune response in the horses receiving allogeneic cells may indicate potential for broader application of this treatment, potentially eliminating the need to harvest cells from the injured individual’s own body.

Cite This Article

APA
Guest DJ, Smith MR, Allen WR. (2008). Monitoring the fate of autologous and allogeneic mesenchymal progenitor cells injected into the superficial digital flexor tendon of horses: preliminary study. Equine Vet J, 40(2), 178-181. https://doi.org/10.2746/042516408X276942

Publication

Equine veterinary journal
ISSN: 0425-1644
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 40
Issue: 2
Pages: 178-181

Researcher Affiliations

Guest, D J
  • University of Cambridge, Department of Veterinary Medicine Equine Fertility Unit, Mertoun Paddocks, Woodditton Road, Newmarket, Suffolk CB8 9BH, UK.
Smith, M R W
    Allen, W R

      MeSH Terms

      • Animals
      • Green Fluorescent Proteins
      • Horse Diseases / therapy
      • Horses / injuries
      • Mesenchymal Stem Cell Transplantation / methods
      • Mesenchymal Stem Cell Transplantation / veterinary
      • Mesenchymal Stem Cells / pathology
      • Tendon Injuries / therapy
      • Tendon Injuries / veterinary
      • Tendons
      • Transplantation, Autologous
      • Treatment Outcome

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