Abstract: Morphometric information on ambling horses raised in Türkiye remains limited. This study aimed to characterize the morphometric traits of ambling horses according to breeding origin, age, and sex and to provide baseline phenotypic information on the studied population. A total of 102 naturally ambling horses were evaluated, including indigenous horses raised in Bursa ( = 28), indigenous horses raised in Nazilli, Aydın Province ( = 65), and Afghan-origin horses raised in Bursa ( = 9). Morphometric measurements of the head, body, and limbs were recorded, and the effects of breeding origin, age, and sex were analyzed using a general linear model. Significant differences among breeding origins were observed for several morphometric traits ( < 0.05). Afghan-origin horses generally exhibited higher values for several body measurements, particularly withers height, body length, chest depth, chest width, and heart girth, reflecting morphometric differences within the studied sample. Age- and sex-related differences were also observed for several morphometric traits. The strongest correlation was observed between CH and BH ( = 0.920, < 0.001), followed by WH and BH ( = 0.873, < 0.001) and WH and CH ( = 0.860, < 0.001). ITH was also positively correlated with BH ( = 0.825), CH ( = 0.808), and WH ( = 0.744) (all < 0.001). Overall, this study provides baseline morphometric information on ambling horses from different breeding origins raised in Türkiye and contributes to the phenotypic characterization of the studied population. However, because the Afghan-origin group included only nine horses and the distribution of animals among some groups was unbalanced, the observed differences should be interpreted with caution. Further studies involving larger and more balanced populations are needed to confirm these findings.
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Overview
This study provides a detailed physical (morphometric) characterization of ambling horses from different breeding origins in Türkiye, analyzing how factors such as breeding origin, age, and sex influence their body measurements.
The research establishes baseline phenotypic data for these horses, identifying significant differences within the population and correlations between various body traits.
Introduction and Objective
The study addresses the limited information available on the morphometric traits of ambling horses raised in Türkiye.
The main objective was to characterize the physical traits of these horses based on breeding origin (location and type), age, and sex.
The study also aimed to provide baseline phenotypic data to help with future research and conservation efforts.
Study Population and Data Collection
A total of 102 naturally ambling horses were examined.
Three groups were identified based on breeding origin:
Indigenous horses raised in Bursa (28 horses)
Indigenous horses raised in Nazilli, Aydın Province (65 horses)
Afghan-origin horses raised in Bursa (9 horses)
Measurements were taken on various body parts, including the head, body, and limbs, to capture comprehensive morphological data.
Methods and Analysis
A general linear model was used to analyze the effects of breeding origin, age, and sex on morphometric traits.
Statistical significance was set at p < 0.05 to identify meaningful differences.
Correlations between specific body measurements were calculated to understand relationships among traits.
Main Findings
Significant differences were found among horses from different breeding origins for several morphometric traits.
Afghan-origin horses generally exhibited larger body measurements, including:
Withers height (WH)
Body length (BL)
Chest depth (CD)
Chest width (CW)
Heart girth (HG)
Age and sex also influenced various morphometric traits, with some differences observed between male and female horses and among age groups.
The strongest correlations among traits were:
Chest height (CH) and body height (BH): r = 0.920, p < 0.001
Withers height (WH) and body height (BH): r = 0.873, p < 0.001
Withers height (WH) and chest height (CH): r = 0.860, p < 0.001
Inner thigh height (ITH) with body height (BH), chest height (CH), and withers height (WH) showing correlations of 0.825, 0.808, and 0.744 respectively (all p < 0.001)
Interpretation and Importance
This research highlights morphometric variability within ambling horses raised in different regions of Türkiye, reflecting genetic or environmental differences related to breeding origin.
The established morphometric profiles contribute important baseline data for future breeding programs, conservation, and identification of breeds.
The strong correlations between several body measurements suggest consistent patterns of growth and conformation within the population.
Limitations
Small sample size of the Afghan-origin group (only 9 horses), which limits the generalizability of findings related to this subgroup.
Unequal distribution across groups—indigenous horses from Nazilli had a larger sample size compared to other groups—potentially impacting statistical robustness.
The findings should therefore be interpreted with caution due to these sample imbalances.
Recommendations for Future Research
Further studies with larger and more balanced sample sizes across breeding origins are necessary to confirm and expand upon these findings.
Additional genetic and environmental assessments could help explain the differences observed in morphometric traits.
Longitudinal studies may clarify how age-related changes affect these horses over time.