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Home / Equine Research Bank / Stem Cells Int / MRI-Based Assessment of Intralesional Delivery of Bone Marro...
Stem cells international2016; 2016; 8610964; doi: 10.1155/2016/8610964

MRI-Based Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem Cells in a Model of Equine Tendonitis.

Abstract: Ultrasound-guided intralesional injection of mesenchymal stem cells (MSCs) is held as the benchmark for cell delivery in tendonitis. The primary objective of this study was to investigate the immediate cell distribution following intralesional injection of MSCs. Unilateral superficial digital flexor tendon (SDFT) lesions were created in the forelimb of six horses and injected with 10 × 10 MSCs labeled with superparamagnetic iron oxide nanoparticles (SPIOs) under ultrasound guidance. Assays were performed to confirm that there were no significant changes in cell viability, proliferation, migration, or trilineage differentiation due to the presence of SPIOs. Limbs were imaged on a 1.5-tesla clinical MRI scanner postmortem before and after injection to determine the extent of tendonitis and detect SPIO MSCs. Clusters of labeled cells were visible as signal voids in 6/6 subjects. Coalescing regions of signal void were diffusely present in the peritendinous tissues. Although previous reports have determined that local injury retains cells within a small radius of the site of injection, our study shows greater than expected delocalization and relatively few cells retained within collagenous tendon compared to surrounding fascia. Further work is needed if this is a reality and to determine if directed intralesional delivery of MSCs is as critical as presently thought.
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Publication Date: 2016-09-26 PubMed ID: 27746821PubMed Central: PMC5056306DOI: 10.1155/2016/8610964Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article looks into the effectiveness of intralesional injection of mesenchymal stem cells as a treatment for tendonitis in horses. The researchers used ultrasound guidance to inject the cells and then performed MRI scans to assess the distribution and viability of the cells.

Introduction to the Study

  • The research aimed to understand more about the distribution and behavior of mesenchymal stem cells (MSCs) when injected directly into the site of tendon injuries in horses.
  • The authors created tendon lesions in the forelimbs of six horses and injected these with MSCs, which were tracked using MRI.

Methodology of the Study

  • Mesenchymal stem cells were labelled with superparamagnetic iron oxide nanoparticles (SPIOs), which allow the cells to be seen on an MRI scan.
  • The researchers firstly confirmed that the use of SPIO markers did not significantly affect the cells’ viability, proliferation, migration, or ability to differentiate into multiple cell lines.
  • Once the MSCs were injected into the tendon lesions, the horses were imaged with a clinical MRI scanner to determine the extent of the tendonitis and locate the SPIO-labelled MSCs.

Findings of the Study

  • The researchers found clusters of the labelled cells visible as signal voids in all subjects, indicating successful injection and distribution of the MSCs.
  • The authors noticed larger than expected signal void regions appearing diffusely in the peritendinous tissues, suggesting that the MSCs had migrated away from the lesion sites more than anticipated.
  • This calls into question prevailing wisdom that local injury tends to retain cells within a small radius.
  • The study showed that fewer MSCs remained within the collagenous tendon compared to the surrounding fascia.

Conclusion of the Study

  • Based on these findings, the authors deduced that the targeted intralesional delivery of MSCs may not be as crucial as previously believed.
  • However, they noted that further research is needed to confirm this observation and to thoroughly understand cell delocalization in this context.

Cite This Article

APA
Scharf A, Holmes SP, Thoresen M, Mumaw J, Stumpf A, Peroni J. (2016). MRI-Based Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem Cells in a Model of Equine Tendonitis. Stem Cells Int, 2016, 8610964. https://doi.org/10.1155/2016/8610964

Publication

Stem cells international
ISSN: 1687-966X
NlmUniqueID: 101535822
Country: United States
Language: English
Volume: 2016
Pages: 8610964
PII: 8610964

Researcher Affiliations

Scharf, Alexandra
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA; Department of Biological and Agricultural Engineering, College of Engineering, University of Georgia, Athens, GA 30602, USA.
Holmes, Shannon P
  • Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Thoresen, Merrilee
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Mumaw, Jennifer
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Stumpf, Alaina
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Peroni, John
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.

Conflict of Interest Statement

The authors declare that there are no competing interests regarding the publication of this paper.

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