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Home / Equine Research Bank / Differentiation / Multilineage differentiation potential of equine blood-deriv...
Differentiation; research in biological diversity2007; 76(2); 118-129; doi: 10.1111/j.1432-0436.2007.00207.x

Multilineage differentiation potential of equine blood-derived fibroblast-like cells.

Abstract: Tissue engineering (TE) has emerged as a promising new therapy for the treatment of damaged tissues and organs. Adult stem cells are considered as an attractive candidate cell type for cell-based TE. Mesenchymal stem cells (MSC) have been isolated from a variety of tissues and tested for differentiation into different cell lineages. While clinical trials still await the use of human MSC, horse tendon injuries are already being treated with autologous bone marrow-derived MSC. Given that the bone marrow is not an optimal source for MSC due to the painful and risk-containing sampling procedure, isolation of stem cells from peripheral blood would bring an attractive alternative. Adherent fibroblast-like cells have been previously isolated from equine peripheral blood. However, their responses to the differentiation conditions, established for human bone marrow MSC, were insufficient to fully confirm their multilineage potential. In this study, differentiation conditions were optimized to better evaluate the multilineage capacities of equine peripheral blood-derived fibroblast-like cells (ePB-FLC) into adipogenic, osteogenic, and chondrogenic pathways. Adipogenic differentiation using rabbit serum resulted in a high number of large-size lipid droplets three days upon induction. Cells' expression of alkaline phosphatase and calcium deposition upon osteogenic induction confirmed their osteogenic differentiation capacities. Moreover, an increase of dexamethasone concentration resulted in faster osteogenic differentiation and matrix mineralization. Finally, induction of chondrogenesis in pellet cultures resulted in an increase in cartilage-specific gene expression, namely collagen II and aggrecan, followed by protein deposition after a longer induction period. This study therefore demonstrates that ePB-FLC have the potential to differentiate into adipogenic, osteogenic, and chondrogenic mesenchymal lineages. The presence of cells with confirmed multilineage capacities in peripheral blood has important clinical implications for cell-based TE therapies in horses.
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Publication Date: 2007-08-14 PubMed ID: 17697129DOI: 10.1111/j.1432-0436.2007.00207.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research gives insight into the potential of equine peripheral blood-derived fibroblast-like cells (ePB-FLC) as potential candidates for tissue engineering in veterinary medicine, showing this cell type can be coaxed to specialize into adipogenic, osteogenic, and chondrogenic lineages suitable for treating injuries and tissue damage in horses.

Background

  • The field of tissue engineering (TE) is exploring new avenues for treatment of damaged tissues and organs. This often involves the use of adult stem cells, particularly mesenchymal stem cells (MSC), due to their ability to differentiate into a variety of tissue types.
  • In veterinary medicine, there has been success treating horse tendon injuries with MSCs derived from the horse’s own bone marrow. However, this method of collection can be painful and potentially risky.
  • Researchers have therefore been exploring the use of fibroblast-like cells derived from equine peripheral blood (ePB-FLC) as an alternative source of MSCs. Previous research has hinted at the potential of these cells, but conditions for their differentiation had not been optimized yet.

Research Methods and Findings

  • In the present study, the researchers worked on refining the conditions for differentiating ePB-FLC into several mesenchymal lineages: adipogenic (fat cells), osteogenic (bone cells) and chondrogenic (cartilage cells).
  • They found that inducing adipogenic differentiation with rabbit serum resulted in the rapid formation of large lipid droplets, confirming successful differentiation into fat cells.
  • Similarly, the cells were capable of osteogenic differentiation, as evidenced by the expression of alkaline phosphatase and the deposition of calcium. Importantly, it was found that increasing the concentration of dexamethasone, a steroid, resulted in faster and more robust osteogenic differentiation and matrix mineralization.
  • Lastly, chondrogenic differentiation was evident in pellet cultures, with an increase in the expression of cartilage-specific genes like collagen II and aggrecan, plus protein deposition after an extended period of induction.

Significance of the Study

  • The ability of ePB-FLC to differentiate into adipogenic, osteogenic and chondrogenic cells suggests that these could serve as viable MSC alternatives for cell-based TE treatment in horses.
  • This is particularly relevant in contexts where bone marrow collection is impractical or undesirable, and it could possibly pave the way for less invasive and more effective treatments for tissue damage in veterinary medicine.

Cite This Article

APA
Giovannini S, Brehm W, Mainil-Varlet P, Nesic D. (2007). Multilineage differentiation potential of equine blood-derived fibroblast-like cells. Differentiation, 76(2), 118-129. https://doi.org/10.1111/j.1432-0436.2007.00207.x

Publication

Differentiation; research in biological diversity
ISSN: 1432-0436
NlmUniqueID: 0401650
Country: England
Language: English
Volume: 76
Issue: 2
Pages: 118-129

Researcher Affiliations

Giovannini, Samoa
  • Institute for Pathology, Osteoarticular Research Group, University of Bern, Bern, Switzerland. pierre.mainil@pathology.unibe.ch
Brehm, Walter
    Mainil-Varlet, Pierre
      Nesic, Dobrila

        MeSH Terms

        • Adipogenesis
        • Animals
        • Blood Cells / cytology
        • Cell Culture Techniques
        • Cell Differentiation
        • Cell Separation
        • Chondrogenesis
        • Horses
        • Mesenchymal Stem Cells / cytology
        • Osteogenesis

        Citations

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