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Home / Equine Research Bank / Vet Parasitol / Muscle distribution of sylvatic and domestic Trichinella lar...
Veterinary parasitology2005; 132(1-2); 101-105; doi: 10.1016/j.vetpar.2005.05.036

Muscle distribution of sylvatic and domestic Trichinella larvae in production animals and wildlife.

Abstract: Only a few studies have compared the muscle distribution of the different Trichinella genotypes. In this study, data were obtained from a series of experimental infections in pigs, wild boars, foxes and horses, with the aim of evaluating the predilection sites of nine well-defined genotypes of Trichinella. Necropsy was performed at 5, 10, 20 and 40 weeks post inoculation. From all host species, corresponding muscles/muscle groups were examined by artificial digestion. In foxes where all Trichinella species established in high numbers, the encapsulating species were found primarily in the tongue, extremities and diaphragm, whereas the non-encapsulating species were found primarily in the diaphragm. In pigs and wild boars, only Trichinella spiralis, Trichinella pseudospiralis and Trichinella nelsoni showed extended persistency of muscle larvae (ML), but for all genotypes the tongue and the diaphragm were found to be predilection sites. This tendency was most obvious in light infections. In the horses, T. spiralis, Trichinella britovi, and T. pseudospiralis all established at high levels with predilection sites in the tongue, the masseter and the diaphragm. For all host species, high ML burdens appeared to be more evenly distributed with less obvious predilection than in light infections; predilection site muscles harbored a relatively higher percent of the larval burden in light infections than in heavy infections. This probably reflects increasing occupation of available muscle fibers as larger numbers of worms accumulate. Predilection sites appear to be influenced primarily by host species and secondarily by the age and level of infection.
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Publication Date: 2005-06-28 PubMed ID: 15979801DOI: 10.1016/j.vetpar.2005.05.036Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article examines the distribution of different Trichinella genotypes, a type of parasitic roundworm, in various animal muscle groups. The findings point to the potential influence of host species, age and the level of infection on the preferred sites of Trichinella infection.

Research Objectives and Methodology

  • The aim of the paper is to investigate the preferred sites of nine different Trichinella genotypes in various animals (pigs, wild boars, foxes and horses). The research was achieved by executing a series of experimental infections.
  • The researchers performed necropsies (autopsies on the animals) at 5, 10, 20, and 40 weeks after the animals were innoculated with the Trichinella worms.
  • The researchers examined different muscle groups in each animal species through artificial digestion to determine the distribution of Trichinella larvae.

Key Findings

  • All Trichinella species established in high numbers in foxes. The encapsulating species were mostly found in the tongue, extremities and diaphragm, while non-encapsulating species were primarily found in the diaphragm.
  • In pigs and wild boars, only three Trichinella genotypes (Trichinella spiralis, Trichinella pseudospiralis and Trichinella nelsoni) demonstrated extended persistency of muscle larvae. Regardless of the genotype, the tongue and the diaphragm were the preferred sites.
  • In horses, three different Trichinella species established at high levels, with preferred sites in the tongue, the masseter (jaw muscle) and the diaphragm.
  • Overall, higher muscle larvae burdens were more evenly distributed in the host body, with less clear predilection than in the case of light infections.

Interpreting the Findings

  • The study suggests that the muscle larvae tend to distribute more evenly across the host’s musculature when the infection level is high. On the other hand, in cases of light, or less severe, infections, the larvae seem to prefer certain muscles.
  • The research implies that predilection sites appear to be influenced primarily by host species and secondarily by the age and infection level.

Implications of the Study

  • The research provides crucial insights into the behavior and lifecycle of Trichinella larvae, which can help in understanding how the parasite spreads and persists in different animal hosts. This could be valuable for potential prevention and treatment strategies for trichinellosis, a disease caused by Trichinella infection.

Cite This Article

APA
Kapel CM, Webster P, Gamble HR. (2005). Muscle distribution of sylvatic and domestic Trichinella larvae in production animals and wildlife. Vet Parasitol, 132(1-2), 101-105. https://doi.org/10.1016/j.vetpar.2005.05.036

Publication

Veterinary parasitology
ISSN: 0304-4017
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 132
Issue: 1-2
Pages: 101-105

Researcher Affiliations

Kapel, C M O
  • Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Dyrlaegevej 100, DK1870 Frederiksberg C., Denmark. chj@kvl.dk
Webster, P
    Gamble, H R

      MeSH Terms

      • Animals
      • Foxes / parasitology
      • Horse Diseases / parasitology
      • Horses
      • Larva / growth & development
      • Muscles / parasitology
      • Sus scrofa
      • Swine Diseases / parasitology
      • Trichinella / growth & development
      • Trichinellosis / parasitology
      • Trichinellosis / veterinary

      Citations

      This article has been cited 28 times.
      1. Mohammed MMD, Heikal EA, Ellessy FM, Aboushousha T, Ghareeb MA. Comprehensive chemical profiling of Bassia indica Wight. aerial parts extract using UPLC-ESI-MS/MS, and its antiparasitic activity in Trichinella spiralis infected mice: in silico supported in vivo study. BMC Complement Med Ther 2023 May 18;23(1):161.
        doi: 10.1186/s12906-023-03988-9pubmed: 37202749google scholar: lookup
      2. Veronesi F, Deak G, Diakou A. Wild Mesocarnivores as Reservoirs of Endoparasites Causing Important Zoonoses and Emerging Bridging Infections across Europe. Pathogens 2023 Jan 23;12(2).
        doi: 10.3390/pathogens12020178pubmed: 36839450google scholar: lookup
      3. El-Wakil ES, Shaker S, Aboushousha T, Abdel-Hameed ES, Osman EEA. In vitro and in vivo anthelmintic and chemical studies of Cyperus rotundus L. extracts. BMC Complement Med Ther 2023 Jan 19;23(1):15.
        doi: 10.1186/s12906-023-03839-7pubmed: 36658562google scholar: lookup
      4. Jurkevicz RMB, Silva DAD, Ferreira Neto JM, Matos AMRN, Pires BG, Paschoal ATP, Pinto-Ferreira F, Bracarense APFL, Mitsuka-Breganó R, Freire RL, Navarro IT, Caldart ET. Absence of Trichinella spp. larvae in carcasses of road-killed wild animals in Paraná state, Brazil. Rev Bras Parasitol Vet 2022;31(4):e010622.
        doi: 10.1590/S1984-29612022054pubmed: 36287424google scholar: lookup
      5. G R, G M, P B, L G, G D, C T, G P, S R, Di Donato A, G M, C G, M T. Trichinella surveillance program in wild birds, Emilia-Romagna (northern Italy), 2006-2021. First report of Trichinella pseudospiralis in western marsh harrier (Circus aeruginosus) in Italy. Int J Parasitol Parasites Wildl 2022 Dec;19:191-195.
        doi: 10.1016/j.ijppaw.2022.09.006pubmed: 36213525google scholar: lookup
      6. Eissa FMA, Eassa AHA, Zalat RS, Negm MS, Elmallawany MA. Potential therapeutic effect of platelet-rich plasma and albendazole on the muscular phase of experimental Trichinella spiralis infection. Food Waterborne Parasitol 2022 Sep;28:e00180.
        doi: 10.1016/j.fawpar.2022.e00180pubmed: 36159633google scholar: lookup
      7. Barlow A, Roy K, Hawkins K, Ankarah AA, Rosenthal B. A review of testing and assurance methods for Trichinella surveillance programs. Food Waterborne Parasitol 2021 Sep;24:e00129.
        doi: 10.1016/j.fawpar.2021.e00129pubmed: 34458599google scholar: lookup
      8. Gondek M, Knysz P, Pyz-Łukasik R, Łukomska A, Kuriga A, Pomorska-Mól M. Distribution of Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis in the Diaphragms and T. spiralis and T. britovi in the Tongues of Experimentally Infected Pigs. Front Vet Sci 2021;8:696284.
        doi: 10.3389/fvets.2021.696284pubmed: 34239917google scholar: lookup
      9. Ricchiuti L, Petrini A, Interisano M, Ruberto A, Salucci S, Marino L, Del Riccio A, Cocco A, Badagliacca P, Pozio E. First report of Trichinella pseudospiralis in a wolf (Canis lupus italicus). Int J Parasitol Parasites Wildl 2021 Aug;15:195-198.
        doi: 10.1016/j.ijppaw.2021.05.002pubmed: 34136345google scholar: lookup
      10. La Grange LJ, Mukaratirwa S. Experimental infection of tigerfish (Hydrocynus vittatus) and African sharp tooth catfish (Clarias gariepinus) with Trichinella zimbabwensis. Onderstepoort J Vet Res 2020 Nov 5;87(1):e1-e5.
        doi: 10.4102/ojvr.v87i1.1876pubmed: 33179950google scholar: lookup
      11. La Grange LJ, Mukaratirwa S. Epidemiology and hypothetical transmission cycles of Trichinella infections in the Greater Kruger National Park of South Africa: an example of host-parasite interactions in an environment with minimal human interactions. Parasite 2020;27:13.
        doi: 10.1051/parasite/2020010pubmed: 32163031google scholar: lookup
      12. Gajadhar AA, Noeckler K, Boireau P, Rossi P, Scandrett B, Gamble HR. International Commission on Trichinellosis: Recommendations for quality assurance in digestion testing programs for Trichinella. Food Waterborne Parasitol 2019 Sep;16:e00059.
        doi: 10.1016/j.fawpar.2019.e00059pubmed: 32095629google scholar: lookup
      13. Scandrett B, Konecsni K, Lalonde L, Boireau P, Vallée I. Detection of natural Trichinella murrelli and Trichinella spiralis infections in horses by routine post-slaughter food safety testing. Food Waterborne Parasitol 2018 Jun;11:1-5.
        doi: 10.1016/j.fawpar.2018.06.001pubmed: 32095599google scholar: lookup
      14. Gajadhar A, Konecsni K, Scandrett B, Buholzer P. Validation of a new commercial serine protease artificial digestion assay for the detection of Trichinella larvae in pork. Food Waterborne Parasitol 2018 Mar;10:6-13.
        doi: 10.1016/j.fawpar.2018.04.001pubmed: 32095596google scholar: lookup
      15. Gondek M, Herosimczyk A, Knysz P, Ożgo M, Lepczyński A, Szkucik K. Comparative Proteomic Analysis of Serum from Pigs Experimentally Infected with Trichinella spiralis, Trichinella britovi, and Trichinella pseudospiralis. Pathogens 2020 Jan 11;9(1).
        doi: 10.3390/pathogens9010055pubmed: 31940868google scholar: lookup
      16. Otranto D, Deplazes P. Zoonotic nematodes of wild carnivores. Int J Parasitol Parasites Wildl 2019 Aug;9:370-383.
        doi: 10.1016/j.ijppaw.2018.12.011pubmed: 31338295google scholar: lookup
      17. Sharma R, Thompson P, Elkin B, Mulders R, Branigan M, Pongracz J, Wagner B, Scandrett B, Hoberg E, Rosenthal B, Jenkins E. Trichinella pseudospiralis in a wolverine (Gulo gulo) from the Canadian North. Int J Parasitol Parasites Wildl 2019 Aug;9:274-280.
        doi: 10.1016/j.ijppaw.2019.06.005pubmed: 31289720google scholar: lookup
      18. Kärssin A, Häkkinen L, Niin E, Peik K, Vilem A, Jokelainen P, Lassen B. Trichinella spp. biomass has increased in raccoon dogs (Nyctereutes procyonoides) and red foxes (Vulpes vulpes) in Estonia. Parasit Vectors 2017 Dec 16;10(1):609.
        doi: 10.1186/s13071-017-2571-0pubmed: 29246168google scholar: lookup
      19. Riva E, Fiel C, Bernat G, Muchiut S, Steffan P. Studies on vertical transmission of Trichinella spiralis in experimentally infected guinea pigs (Cavia porcellus). Parasitol Res 2017 Aug;116(8):2271-2276.
        doi: 10.1007/s00436-017-5533-8pubmed: 28631164google scholar: lookup
      20. Mayer-Scholl A, Pozio E, Gayda J, Thaben N, Bahn P, Nöckler K. Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples. J Vis Exp 2017 Mar 3;(121).
        doi: 10.3791/55354pubmed: 28287594google scholar: lookup
      21. Roesel K, Nöckler K, Baumann MP, Fries R, Dione MM, Clausen PH, Grace D. First Report of the Occurrence of Trichinella-Specific Antibodies in Domestic Pigs in Central and Eastern Uganda. PLoS One 2016;11(11):e0166258.
        doi: 10.1371/journal.pone.0166258pubmed: 27870858google scholar: lookup
      22. Riehn K, Hamedy A, Grosse K, Zeitler L, Lücker E. A novel detection method for Alaria alata mesocercariae in meat. Parasitol Res 2010 Jun;107(1):213-20.
        doi: 10.1007/s00436-010-1853-7pubmed: 20405145google scholar: lookup
      23. Meng XJ, Lindsay DS, Sriranganathan N. Wild boars as sources for infectious diseases in livestock and humans. Philos Trans R Soc Lond B Biol Sci 2009 Sep 27;364(1530):2697-707.
        doi: 10.1098/rstb.2009.0086pubmed: 19687039google scholar: lookup
      24. Gottstein B, Pozio E, Nöckler K. Epidemiology, diagnosis, treatment, and control of trichinellosis. Clin Microbiol Rev 2009 Jan;22(1):127-45, Table of Contents.
        doi: 10.1128/CMR.00026-08pubmed: 19136437google scholar: lookup
      25. Davidson RK, Handeland K, Kapel CM. High tolerance to repeated cycles of freezing and thawing in different Trichinella nativa isolates. Parasitol Res 2008 Oct;103(5):1005-10.
        doi: 10.1007/s00436-008-1079-0pubmed: 18594862google scholar: lookup
      26. Hagras NA, Hegab F, Atta S, Gadallah RA, Elsayed Y, Khodear GAM. Promising therapeutic efficacy of nitazoxanide-loaded zinc oxide nano-formula against intestinal and muscular phases of experimental trichinellosis. PLoS Negl Trop Dis 2025 Jul;19(7):e0013239.
        doi: 10.1371/journal.pntd.0013239pubmed: 40679976google scholar: lookup
      27. Mohamed AM, Hegab AM, Amer SII, Zalat RS, Shoeib EY, Abdel-Shafi IR. Evaluation of the prophylactic and therapeutic effects of selenium and vitamin E on the muscular phase of experimental Trichinella spiralis infection in mice. J Parasit Dis 2025 Jun;49(2):378-388.
        doi: 10.1007/s12639-024-01762-3pubmed: 40458510google scholar: lookup
      28. Allam AF, Mostafa RA, Lotfy W, Farag HF, Fathi N, Moneer EA, Shehab AY. Therapeutic efficacy of mebendazole and artemisinin in different phases of trichinellosis: a comparative experimental study. Parasitology 2021 Apr;148(5):630-635.
        doi: 10.1017/S0031182021000056pubmed: 33517933google scholar: lookup
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