Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina.
Abstract: Infection with Equid Herpesvirus type 1 (EHV-1) leads to respiratory disease, abortion, and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A(2254)/G(2254)) in the genome region of the open reading frame 30 (ORF30), which results in an amino acid variation (N(752)/D(752)) of the EHV-1 DNA polymerase, is significantly associated with the neuropathogenic potential of naturally occurring strains. In order to estimate the prevalence of the EHV-1 neuropathogenic genotype in our country, we analyzed the ORF30 genome region of Argentinean EHV-1 isolates. The study was carried out by real time allelic discrimination PCR in 90 equine EHV-1-positive samples, being 89 from 54 cases of abortion outbreaks (two of which were in association with neurological disease) and one from the respiratory tract of a healthy horse in training. Our results indicate that 7% (4/54) of the abortion outbreaks studied were induced by the neuropathogenic (G(2254)) genotype of EHV-1 and 50% (2/4) of them were associated with simultaneous neurological disease. This information emphasizes the necessity to extreme the hygienic and preventive measures to diminish EHV-1 infections and consequently reduce the risk of epizootic neurological disease as has been recently observed in other countries.
Publication Date: 2009-06-21 PubMed ID: 19589651DOI: 10.1016/j.vetmic.2009.06.025Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study analyzes the prevalence of neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 (EHV-1) in Argentina. The research finds that a small percentage of abortion outbreaks in horses were caused by a particular genotype of EHV-1, which is known to affect the nervous system.
Overview of the Research
- The research focuses on the infection caused by Equid Herpesvirus type 1 (EHV-1) in horses which leads to numerous health conditions including respiratory disease, abortion, and neurological disorders.
- Previous molecular epidemiology studies conducted on EHV-1 have observed that a single nucleotide polymorphism in the genome region of the open reading frame 30 (ORF30) in the virus is a significant indicator of the neuropathogenic potential of naturally occurring strains. This polymorphism causes an amino acid variation in the EHV-1 DNA polymerase.
Research Methodology
- The research analyzes the ORF30 genome region of Argentinean EHV-1 isolates to ascertain the prevalence of the neuropathogenic genotype of the virus in the country.
- A total of 90 EHV-1-positive samples from horses were examined using real time allelic discrimination PCR. Out of these, 89 samples were taken from 54 cases of abortion outbreaks, including two which were linked to neurological diseases, and one from a healthy horse’s respiratory system.
Research Findings
- This research indicates that out of the examined abortion outbreaks, 7% (4 out of 54) were caused by the neuropathogenic genotype (G(2254)) of EHV-1.
- 50% (2 out of 4) of these cases induced by the neuropathogenic genotype were associated with a simultaneous occurrence of a neurological disease.
Implications
- The results highlight the need for improved hygienic and preventative measures to reduce infections from EHV-1. This can help mitigate the risk of epizootic neurological disease, similar to occurrences noted in other countries.
Cite This Article
APA
Vissani MA, Becerra ML, Olguín Perglione C, Tordoya MS, Miño S, Barrandeguy M.
(2009).
Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina.
Vet Microbiol, 139(3-4), 361-364.
https://doi.org/10.1016/j.vetmic.2009.06.025 Publication
Researcher Affiliations
- Instituto de Virología, CICVyA, INTA. Las Cabañas y los Reseros s/n, (1712) Castelar, Buenos Aires, Argentina. avissani@cnia.inta.gov.ar
MeSH Terms
- Animals
- Argentina / epidemiology
- DNA, Viral / genetics
- Disease Outbreaks / veterinary
- Female
- Genotype
- Herpesviridae Infections / epidemiology
- Herpesviridae Infections / veterinary
- Herpesviridae Infections / virology
- Herpesvirus 1, Equid / genetics
- Herpesvirus 1, Equid / isolation & purification
- Horse Diseases / epidemiology
- Horse Diseases / virology
- Horses
- Mice
- Polymerase Chain Reaction / veterinary
- Pregnancy
Citations
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