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Home / Equine Research Bank / Animals (Basel) / Neutrophil Gelatinase-Associated Lipocalin in Synovial Fluid...
Animals : an open access journal from MDPI2022; 13(1); 29; doi: 10.3390/ani13010029

Neutrophil Gelatinase-Associated Lipocalin in Synovial Fluid from Horses with and without Septic Arthritis.

Abstract: Neutrophil gelatinase-associated lipocalin (NGAL) has been suggested to be a highly sensitive and specific marker of joint infection in humans. The aim of the study was to investigate NGAL concentrations in synovial fluid (SF) from horses with septic synovitis, horses without septic synovitis, and horses with uncertain status. NGAL was measured in 177 admission samples obtained from 152 horses. From a subset of horses ( = 35), additional samples obtained sequentially over the course of treatment were available. Concentrations of NGAL were significantly higher in septic synovitis ( = 47 samples) than in samples classified as non-septic ( = 103) or samples with uncertain status ( = 27), with median NGAL concentrations in the three groups being 1236, 16.8, and 266.4 µg/L, respectively. NGAL discriminated nearly perfectly between septic and non-septic (area under the receiver operating characteristic curve 0.98, 95% confidence interval 0.95-1.00). The optimal cut-off value for maximal sensitivity (87.2%) and specificity (75.0%) to discriminate septic samples from those with uncertain status was 444.6 µg/L, with an area under the receiver operating characteristic curve of 0.85 (95% confidence interval 0.74-0.93). Concentrations declined over time in horses undergoing treatment. NGAL is a novel biomarker that seems to have great potential for identifying septic synovitis and for monitoring the response to treatment of synovial infection in horses.
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Publication Date: 2022-12-21 PubMed ID: 36611638PubMed Central: PMC9817893DOI: 10.3390/ani13010029Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focused on evaluating the effectiveness of Neutrophil Gelatinase-Associated Lipocalin (NGAL) as a marker for joint infection in horses. It found that NGAL concentrations were significantly higher in horses with septic synovitis and the levels decreased over time with treatment.

Objectives of the Research

  • The primary aim of the study was to investigate the levels of NGAL in the synovial fluid of horses suffering from septic synovitis and compare these with horses not suffering from the condition, as well as horses with an uncertain status.
  • The researchers also sought to determine whether NGAL could serve as a valuable biomarker in the identification of septic synovitis, as well as in monitoring the effectiveness of treatment for the condition.

Methods and Procedures

  • The study examined 177 admission samples obtained from 152 horses.
  • From a subset of 35 horses, additional samples were secured over the course of treatment.
  • The study measured and compared the concentrations of NGAL in samples classified as septic synovitis with samples categorized as non-septic and samples with an uncertain status.
  • The researchers utilized the receiver operating characteristic curve analysis to determine the discriminatory power of NGAL between septic and non-septic samples.

Findings of the Research

  • The findings showed that NGAL concentrations were significantly higher in cases of septic synovitis compared to non-septic samples or those with an uncertain status.
  • NGAL was found to have a near-perfect discriminatory power, with an area under the receiver operating characteristic curve of 0.98, indicating a high level of accuracy in distinguishing between septic and non-septic synovitis.
  • The optimal cut-off value for maximal sensitivity and specificity in distinguishing septic samples from those with an uncertain status was at 444.6 µg/L, which was determined by the area under the receiver operating characteristic curve.
  • It was also observed that the NGAL concentrations reduced over time in horses undergoing treatment for septic synovitis, suggesting that NGAL levels can potentially monitor therapeutic response.

In conclusion, this study positions NGAL as a potentially effective biomarker for detecting and monitoring the treatment of septic synovitis in horses. This could have significant implications for equine veterinary medicine and potentially, treatment of similar conditions in other animals or humans.

Cite This Article

APA
Jacobsen S, Mortensen CD, Høj EA, Vinther AM, Berg LC, Adler DMT, Verwilghen D, van Galen G. (2022). Neutrophil Gelatinase-Associated Lipocalin in Synovial Fluid from Horses with and without Septic Arthritis. Animals (Basel), 13(1), 29. https://doi.org/10.3390/ani13010029

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 1
PII: 29

Researcher Affiliations

Jacobsen, Stine
  • Department of Veterinary Clinical Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark.
Mortensen, Camilla Drejer
  • Department of Veterinary Clinical Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark.
Høj, Elisabeth Alkærsig
  • Department of Veterinary Clinical Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark.
Vinther, Anne Mette
  • BioPorto Diagnostics A/S, DK-3150 Hellerup, Denmark.
Berg, Lise Charlotte
  • Department of Veterinary Clinical Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark.
Adler, Ditte Marie Top
  • Department of Veterinary Clinical Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark.
Verwilghen, Denis
  • Sydney School of Veterinary Science, University of Sydney, Camperdown, Sydney, NSW 2050, Australia.
van Galen, Gaby
  • Sydney School of Veterinary Science, University of Sydney, Camperdown, Sydney, NSW 2050, Australia.

Grant Funding

  • DFF - 7017-00066 / Independent Research Fund Denmark

Conflict of Interest Statement

A.M.V. was employed by Bioporto A/S at the time of the data collection. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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