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Home / Equine Research Bank / Res Vet Sci / Ovine amniotic epithelial cells: in vitro characterization a...
Research in veterinary science2012; 94(1); 158-169; doi: 10.1016/j.rvsc.2012.07.028

Ovine amniotic epithelial cells: in vitro characterization and transplantation into equine superficial digital flexor tendon spontaneous defects.

Abstract: In vitro expanded and frosted ovine amniotic epithelial cells (oAECs) were evaluated for their phenotype, stemness and attitude to differentiate into tenocytes. Fifteen horses with acute tendon lesions were treated with one intralesional injection of oAECs. Tendon recovery under controlled training was monitored. In vitro expanded oAECs showed a constant proliferative ability, a conserved phenotype and stable expression profile of stemness markers. Differentiation into tenocytes was also regularly documented. US controls showed the infilling of the defect and early good alignment of the fibers and 12 horses resumed their previous activity. Histological and immunohistochemical examinations in an explanted tendon demonstrated the low immunogenicity of oAECs that were able to survive in the healing site. In addition, oAECs supported the regenerative process producing ovine collagen type I amongst the equine collagen fibers. Considering our results, oAECs can be proposed as a new approach for the treatment of spontaneous equine tendon injuries.
Copyright © 2012 Elsevier Ltd. All rights reserved.
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Publication Date: 2012-09-03 PubMed ID: 22954787DOI: 10.1016/j.rvsc.2012.07.028Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study observed the potential of using ovine amniotic epithelial cells (oAECs) as treatment for acute tendon lesions in horses. The researchers found that these cells are capable of consistent proliferation and differentiation into tenocytes, essential cells for tendon repair, and contributed positively to tendon healing with low immune response.

Experiment Overview

  • The researchers incubated ovine amniotic epithelial cells (oAECs) in a laboratory setting to evaluate several aspects: their ability to sustain a constant rate of growth (proliferation), maintain their unique cell characteristics (phenotype), exhibit signs of being stem cells (expression of stemness markers), and differentiate into the specific cells that form and repair tendons (tenocytes).
  • The team then tested these cultured oAECs practically by injecting them into horses with spontaneous acute tendon lesions to observe how they fared in real-world conditions.

Results

  • The in-vitro study showed that these oAECs possessed a stable ability to reproduce, maintain their characteristics, and transform into tenocytes, affirming their effectiveness for further analysis.
  • Out of fifteen horses treated with an injection of oAECs, twelve showed favorable tendon recovery under regulated training. This was monitored through ultrasonography, which exhibited these horses’ tendons’ filling of lesions and significant realignment of fibers.
  • Further analyzing an explanted tendon, histological and immunohistochemical examination proved the low immunogenicity of the oAECs, indicating they did not trigger substantial immune response in the horses and were able to endure at the healing location.
  • The oAECs also seemed to facilitate the healing process by producing ovine collagen type I in the midst of the horses’ tendon fibers. Collagen is a crucial component of the tendon, and its production aids in its repair.

Conclusions

  • Given the consistent proliferative ability, differentiation into tenocytes, and low immunogenicity of oAECs in repairing tendon injuries in horses, this study’s authors suggest using oAECs might serve as an effective new approach for managing such harm.
  • The favorable pose of oAECs with respect to the collagen production indicates their capacity not just to survive in the healing environment, but to actively contribute to tissue regeneration.

This research shifts the platform to the potential use of stem cells obtained from amniotic epithelial cells for the treatment of tendon injuries in equine and possibly other species. Further research is needed to confirm these outcomes and assess the broader applications of oAECs in therapeutic treatment.

Cite This Article

APA
Muttini A, Valbonetti L, Abate M, Colosimo A, Curini V, Mauro A, Berardinelli P, Russo V, Cocciolone D, Marchisio M, Mattioli M, Tosi U, Podaliri Vulpiani M, Barboni B. (2012). Ovine amniotic epithelial cells: in vitro characterization and transplantation into equine superficial digital flexor tendon spontaneous defects. Res Vet Sci, 94(1), 158-169. https://doi.org/10.1016/j.rvsc.2012.07.028

Publication

Research in veterinary science
ISSN: 1532-2661
NlmUniqueID: 0401300
Country: England
Language: English
Volume: 94
Issue: 1
Pages: 158-169
PII: S0034-5288(12)00236-6

Researcher Affiliations

Muttini, A
  • Department of Comparative Biomedical Sciences, University of Teramo, Italy. amuttini@unite.it
Valbonetti, L
    Abate, M
      Colosimo, A
        Curini, V
          Mauro, A
            Berardinelli, P
              Russo, V
                Cocciolone, D
                  Marchisio, M
                    Mattioli, M
                      Tosi, U
                        Podaliri Vulpiani, M
                          Barboni, B

                            MeSH Terms

                            • Amnion / cytology
                            • Animals
                            • Cell Differentiation / physiology
                            • Epithelial Cells / cytology
                            • Epithelial Cells / transplantation
                            • Female
                            • Flow Cytometry / veterinary
                            • Horse Diseases / surgery
                            • Horses
                            • In Vitro Techniques
                            • Pregnancy
                            • Reverse Transcriptase Polymerase Chain Reaction / veterinary
                            • Sheep
                            • Tendon Injuries / surgery
                            • Tendon Injuries / veterinary
                            • Tendons / cytology
                            • Tendons / metabolism
                            • Tendons / physiology

                            Citations

                            This article has been cited 25 times.
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