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Home / Equine Research Bank / Eur J Appl Physiol / Oxygen radical absorbance capacity (ORAC) and exercise-induc...
European journal of applied physiology2005; 95(5-6); 550-556; doi: 10.1007/s00421-005-0034-3

Oxygen radical absorbance capacity (ORAC) and exercise-induced oxidative stress in trotters.

Abstract: Strenuous exercise is a potent inducer of oxidative stress, which has been suggested to be associated with disturbances in muscle homeostasis, fatigue and injury. There is no comprehensive or uniform view of the antioxidant status in horses. We have previously shown that moderate exercise induces protein oxidation in trotters. The aim of this study was to measure the antioxidative capacity of the horse in relation to different antioxidant components and oxidative stress markers after a single bout of moderate exercise to elucidate the mechanisms of antioxidant protection in horses. Eight clinically normal and regularly trained standard-bred trotters were treadmill-exercised for 53 min at moderate intensity. Blood samples were collected prior to and immediately after exercise and at 4 and 24 h of recovery. Muscle biopsies from the middle gluteal muscle were taken before exercise and after 4 h of recovery. Acute induction of oxygen radical absorbance capacity (ORAC) did not prevent exercise-induced oxidative stress, which was demonstrated by increased lipid hydroperoxides (LPO). Pre-exercise ORAC levels were, however, a determinant of total glutathione content of the blood after 4 and 24 h of recovery. Furthermore, baseline ORAC level correlated negatively with 4-h recovery LPO levels. Our results imply that horses are susceptible to oxidative stress, but a stronger antioxidant capacity may improve coping with exercise-induced oxidative stress.
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Publication Date: 2005-08-31 PubMed ID: 16136323DOI: 10.1007/s00421-005-0034-3Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates how strenuous exercise impacts oxidative stress in horses, particularly focusing on the antioxidative capacity of a horse. It reveals that stronger antioxidant capacity might help horses deal with oxidative stress induced by exercise.

Background

  • The study explores the relationship between Oxygen Radical Absorbance Capacity (ORAC) and exercise-induced oxidative stress.
  • Exercise is known to be a significant inducer of oxidative stress, which can cause muscle problems, fatigue, and even injury in animals, including horses.

Objective

  • The research aimed to measure the antioxidative capacity of a horse in relation to different antioxidant components and oxidative stress markers after a moderate bout of exercise.
  • This will clarify the mechanisms of antioxidant protection in horses.

Methods

  • The experiment was conducted with eight regular trained standard-bred trotters, which were treadmill-exercised for 53 minutes at a moderate intensity.
  • Blood samples were collected before and immediately after exercise, and during the recovery periods (4 hours and 24 hours after exercise).
  • Muscle biopsies from the horses’ middle gluteal muscle were taken before and 4 hours after the exercise.

Findings

  • The research discovered that the acute induction of ORAC did not prevent exercise-induced oxidative stress, as shown by increased lipid hydroperoxides (LPO).
  • However, pre-exercise ORAC levels were found to be a determinant of total glutathione content of the blood after 4 and 24 hours of recovery.
  • The baseline ORAC level was discovered to have a negative correlation with 4-hour recovery LPO levels, meaning a higher initial ORAC level was associated with lower levels of oxidative stress.

Implications

  • From these results, it was implied that horses are susceptible to oxidative stress. However, having a stronger antioxidant capacity may help the animals adapt better to exercise-induced oxidative stress.
  • These findings might aid in developing a more effective training and health maintenance routine for horses, potentially preventing injury and enhancing performance.

Cite This Article

APA
Kinnunen S, Hyyppä S, Lehmuskero A, Oksala N, Mäenpää P, Hänninen O, Atalay M. (2005). Oxygen radical absorbance capacity (ORAC) and exercise-induced oxidative stress in trotters. Eur J Appl Physiol, 95(5-6), 550-556. https://doi.org/10.1007/s00421-005-0034-3

Publication

European journal of applied physiology
ISSN: 1439-6319
NlmUniqueID: 100954790
Country: Germany
Language: English
Volume: 95
Issue: 5-6
Pages: 550-556

Researcher Affiliations

Kinnunen, Susanna
  • Department of Physiology, University of Kuopio, P.O. Box 1627, Kuopio, FIN-70211, Finland.
Hyyppä, Seppo
    Lehmuskero, Arja
      Oksala, Niku
        Mäenpää, Pekka
          Hänninen, Osmo
            Atalay, Mustafa

              MeSH Terms

              • Animals
              • Antioxidants / metabolism
              • Glutathione / metabolism
              • Horses / metabolism
              • Lipid Peroxides / blood
              • Oxidative Stress / physiology
              • Physical Conditioning, Animal / physiology
              • Reactive Oxygen Species / metabolism
              • Vitamin E / blood

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