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Parenchymal and vascular lesions in ageing equine brains: histological and immunohistochemical studies.
Abstract: Many age-related changes are described in the nervous system of different species, but detailed studies of brain lesions in ageing horses are lacking. The aim of the present study was to systematically characterize lesions in the brains of 60 horses aged from 7 to 23 years. No gross changes were present in any brain. Microscopically, spongiform changes, lipofuscin storage, corpora amylacea, gliosis and satellitosis were common, together with axonal and neuronal swellings. The most important findings were the presence of pseudocalcium-calcium (pCa-Ca) deposits and arterial wall degeneration. Scanning electron microscopical examination of two cases with vascular mineralization revealed marked deposition of an amorphous substance in the vessel walls that was probably formed by a polyanionic protein matrix and a mineral component. Immunohistochemically, numerous axonal spheroids were positively labelled for ubiquitin. No PrPsc was detected in sections with neuronal vacuolation. Neuronal swelling, corpora amylacea, hippocampal Tau-positive neurons and methenamine-positive diffuse (preamyloid) plaques were also detected. Congo red staining failed to detect amyloid deposition. The characterization of age-related lesions in the brains of these horses will allow these changes to be discriminated from pathological processes in future studies. Some lesions described here, including some vascular changes, the presence of diffuse plaques and tau accumulation in hippocampal neurons, have not been described previously in the horse.
Publication Date: 2009-09-09 PubMed ID: 19744668DOI: 10.1016/j.jcpa.2009.07.007Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article explores age-related changes in the brains of horses, focusing particularly on identifying and characterizing lesions. Utilizing a range of microscopic and immunohistochemical techniques, the study charted a number of conditions commonly found in the brains of aging horses, some of which had not been previously described.
Overview of Study and Findings
The authors studied the brains of 60 horses aged from 7 to 23 years. No gross changes were identified in the brain, suggesting that all changes were at the microscopic level. Their key technique involved using histological and immunohistochemical procedures to examine the brains.
- The microscopic study showed a number of common changes, including spongiform alterations, lipofuscin storage, corpora amylacea, gliosis and satellitosis, and axonal and neuronal swellings.
- Pseudocalcium-calcium (pCa-Ca) deposits and arterial wall degeneration were also identified. These are potential indicators of brain disease or damage and have not been previously linked to the aging process in horses’ brains.
- Scanning Electron Microscope (SEM) studies further helped identify an amorphous substance in the arterial walls, thought to be formed of a polyanionic protein matrix and a mineral component. This finding revealed physical alterations in horse brains with age and potential points of disease onset.
- Immunohistochemical investigations revealed a significant occurrence of axonal spheroids, labeled positive for ubiquitin, an important protein linked with degradation and recycling of cellular materials. This could indicate an increase in the degradation process connected with aging.
- Other findings included neuronal swelling, presence of corpora amylacea, hippocampal neurons accumulating Tau protein, and methenamine-positive diffuse plaques. The latter two findings are especially significant because they are linked to neurodegenerative disorders like Alzheimer’s disease in humans.
- Congo red staining was used to search for amyloid deposition, a common feature of many neurodegenerative diseases. However, no such depositions were detected.
Implications and Future Work
The findings from this study add valuable knowledge to our understanding of aging in the horse’s brain. Identifying these microscopic changes could provide the basis for distinguishing age-related changes from pathological conditions in future investigations.
Furthermore, the presence of novel findings, including vascular modifications and plaque formations, opens up new areas of study in equine neurobiology. For better understanding of these observations, further research would be required.
These findings may also contribute to the larger field of general aging and neurobiology research, particularly regarding age-related changes in brain structures and their implications on health and disease.
Cite This Article
APA
Capucchio MT, Márquez M, Pregel P, Foradada L, Bravo M, Mattutino G, Torre C, Schiffer D, Catalano D, Valenza F, Guarda F, Pumarola M.
(2009).
Parenchymal and vascular lesions in ageing equine brains: histological and immunohistochemical studies.
J Comp Pathol, 142(1), 61-73.
https://doi.org/10.1016/j.jcpa.2009.07.007 Publication
Researcher Affiliations
- Department of Animal Pathology, Faculty of Veterinary Medicine, University of Torino, 10095 Grugliasco, Torino, Italy. mariateresa.capucchio@unito.it
MeSH Terms
- Aging / pathology
- Animals
- Blood Vessels / pathology
- Brain / pathology
- Cerebrovascular Circulation
- Female
- Horses
- Immunohistochemistry
- Male
- Microscopy, Electron, Scanning
Citations
This article has been cited 14 times.- Apostolopoulou EP, Raikos N, Vlemmas I, Michaelidis E, Brellou GD. Metallothionein I/II Expression and Metal Ion Levels in Correlation with Amyloid Beta Deposits in the Aged Feline Brain. Brain Sci 2023 Jul 22;13(7).
- Edler MK, Mhatre-Winters I, Richardson JR. Microglia in Aging and Alzheimer's Disease: A Comparative Species Review. Cells 2021 May 8;10(5).
- Prpar Mihevc S, Majdič G. Canine Cognitive Dysfunction and Alzheimer's Disease - Two Facets of the Same Disease?. Front Neurosci 2019;13:604.
- Gołaszewska A, Bik W, Motyl T, Orzechowski A. Bridging the Gap between Alzheimer's Disease and Alzheimer's-like Diseases in Animals. Int J Mol Sci 2019 Apr 3;20(7).
- Seddighi R, Doherty TJ. Anesthesia of the geriatric equine. Vet Med (Auckl) 2012;3:53-64.
- De Biase D, Costagliola A, Pagano TB, Piegari G, Wojcik S, Dziewiątkowski J, Grieco E, Mattace Raso G, Russo V, Papparella S, Paciello O. Amyloid precursor protein, lipofuscin accumulation and expression of autophagy markers in aged bovine brain. BMC Vet Res 2017 Apr 13;13(1):102.
- Vallino Costassa E, Fiorini M, Zanusso G, Peletto S, Acutis P, Baioni E, Maurella C, Tagliavini F, Catania M, Gallo M, Faro ML, Chieppa MN, Meloni D, D'Angelo A, Paciello O, Ghidoni R, Tonoli E, Casalone C, Corona C. Characterization of Amyloid-β Deposits in Bovine Brains. J Alzheimers Dis 2016;51(3):875-87.
- Chambers JK, Tokuda T, Uchida K, Ishii R, Tatebe H, Takahashi E, Tomiyama T, Une Y, Nakayama H. The domestic cat as a natural animal model of Alzheimer's disease. Acta Neuropathol Commun 2015 Dec 10;3:78.
- Jeffrey M, Piccardo P, Ritchie DL, Ironside JW, Green AJ, McGovern G. A Naturally Occurring Bovine Tauopathy Is Geographically Widespread in the UK. PLoS One 2015;10(6):e0129499.
- Chambers JK, Uchida K, Harada T, Tsuboi M, Sato M, Kubo M, Kawaguchi H, Miyoshi N, Tsujimoto H, Nakayama H. Neurofibrillary tangles and the deposition of a beta amyloid peptide with a novel N-terminal epitope in the brains of wild Tsushima leopard cats. PLoS One 2012;7(10):e46452.
- Ford M, Gunn-Moore FJ, Dagleish MP. Spontaneous mammalian models for Alzheimer's disease and dementia. Brain Commun 2025;7(4):fcaf287.
- Tan Yi Shean L, Milne EM, Shaw DJ, Maxwell S, Del-Pozo J. Lipofuscin accumulates in ganglionic neurons in chronic equine dysautonomia. J Vet Diagn Invest 2024 Nov;36(6):864-869.
- Takahashi N, Chambers JK, Ota K, Toda K, Yoshimoto Y, Miyajima U, Uchida K. Pituitary pars intermedia dysfunction with pituitary gland melanotroph adenoma in a Grevy's zebra (Equus grevyi). J Vet Med Sci 2024 Jan 10;86(1):71-76.
- Li H, Xiang BL, Li X, Li C, Li Y, Miao Y, Ma GL, Ma YH, Chen JQ, Zhang QY, Lv LB, Zheng P, Bi R, Yao YG. Cognitive Deficits and Alzheimer's Disease-Like Pathologies in the Aged Chinese Tree Shrew. Mol Neurobiol 2024 Apr;61(4):1892-1906.
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