FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Animals (Basel) / Peptide Mediated Adhesion to Beta-Lactam Ring of Equine Mese...
Animals : an open access journal from MDPI2022; 12(6); 734; doi: 10.3390/ani12060734

Peptide Mediated Adhesion to Beta-Lactam Ring of Equine Mesenchymal Stem Cells: A Pilot Study.

Abstract: Regenerative medicine applied to skin lesions is a field in constant improvement. The use of biomaterials with integrin agonists could promote cell adhesion increasing tissue repair processes. The aim of this pilot study was to analyze the effect of an α4β1 integrin agonist on cell adhesion of equine adipose tissue (AT) and Wharton's jelly (WJ) derived MSCs and to investigate their adhesion ability to GM18 incorporated poly L-lactic acid (PLLA) scaffolds. Adhesion assays were performed after culturing AT- and WJ-MSCs with GM18 coating or soluble GM18. Cell adhesion on GM18 containing PLLA scaffolds after 20 min co-incubation was assessed by HCS. Soluble GM18 affects the adhesion of equine AT- and WJ-MSCs, even if its effect is variable between donors. Adhesion to PLLA scaffolds containing GM18 is not significantly influenced by GM18 for AT-MSCs after 20 min or 24 h of culture and for WJ-MSCs after 20 min, but increased cell adhesion by 15% GM18 after 24 h. In conclusion, the α4β1 integrin agonist GM18 affects equine AT- and WJ-MSCs adhesion ability with a donor-related variability. These preliminary results represent a first step in the study of equine MSCs adhesion to PLLA scaffolds containing GM18, suggesting that WJ-MSCs might be more suitable than AT-MSCs. However, the results need to be confirmed by increasing the number of samples before drawing definite conclusions.
Get Full Text
Publication Date: 2022-03-15 PubMed ID: 35327131PubMed Central: PMC8944785DOI: 10.3390/ani12060734Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the use of an α4β1 integrin agonist on the cell adhesion of equine adipose tissue and Wharton’s jelly-derived Mesenchymal Stem Cells (MSCs) to improve tissue repair processes in the field of regenerative medicine. The findings suggest that the α4β1 integrin agonist GM18 can variably affect cell adhesion ability, indicating that further research is needed.

Objective of the Research

  • This pilot study was aimed at understanding how an α4β1 integrin agonist (GM18) could impact cell adhesion of equine adipose tissue (AT) and Wharton’s jelly (WJ) derived MSCs.
  • The researchers also sought to investigate the adhesion ability of these stem cells to GM18 incorporated poly L-lactic acid (PLLA) scaffolds.

Methodology of the Research

  • The team performed adhesion assays after culturing AT and WJ-MSCs with a coating of GM18 or a solution of GM18.
  • The cell adhesion on GM18 containing PLLA scaffolds was assessed after a co-incubation period of 20 minutes through high-content screening (HCS).

Findings of the Research

  • The researchers observed that soluble GM18 affects the adhesion of both equine AT and WJ-MSCs, with the impact varying between donors.
  • Adhesion to PLLA scaffolds containing GM18 does not significantly change for AT-MSCs after either 20 minutes or 24 hours of culture, or for WJ-MSCs after 20 minutes.
  • However, there was an increase in cell adhesion by 15% for GM18 with WJ-MSCs after 24 hours.

Conclusion of the Research

  • Overall, GM18, an α4β1 integrin agonist, appears to impact the adhesion ability of equine AT and WJ-MSCs with variability that depends on the donor.
  • The preliminary results point towards WJ-MSCs potentially being more suitable than AT-MSCs for adhesion to PLLA scaffolds containing GM18.
  • Despite these initial findings, the researchers acknowledge that the results need to be confirmed through additional testing with a greater number of samples before any conclusive arguments can be made.

Cite This Article

APA
(2022). Peptide Mediated Adhesion to Beta-Lactam Ring of Equine Mesenchymal Stem Cells: A Pilot Study. Animals (Basel), 12(6), 734. https://doi.org/10.3390/ani12060734

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 6
PII: 734

Researcher Affiliations

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 44 references
  1. Kashpur O, Smith A, Gerami-Naini B, Maione AG, Calabrese R, Tellechea A, Theocharidis G, Liang L, Pastar I, Tomic-Canic M, Mooney D, Veves A, Garlick JA. Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.. FASEB J 2019 Jan;33(1):1262-1277.
    doi: 10.1096/fj.201801059pmc: PMC6355091pubmed: 30088952google scholar: lookup
  2. Lawrence WT. Physiology of the acute wound.. Clin Plast Surg 1998 Jul;25(3):321-40.
    doi: 10.1016/S0094-1298(20)32467-6pubmed: 9696896google scholar: lookup
  3. Mogoşanu GD, Grumezescu AM. Natural and synthetic polymers for wounds and burns dressing.. Int J Pharm 2014 Mar 25;463(2):127-36.
    doi: 10.1016/j.ijpharm.2013.12.015pubmed: 24368109google scholar: lookup
  4. Gurtner GC, Chapman MA. Regenerative Medicine: Charting a New Course in Wound Healing.. Adv Wound Care (New Rochelle) 2016 Jul 1;5(7):314-328.
    doi: 10.1089/wound.2015.0663pmc: PMC4900191pubmed: 27366592google scholar: lookup
  5. Gorecka J, Kostiuk V, Fereydooni A, Gonzalez L, Luo J, Dash B, Isaji T, Ono S, Liu S, Lee SR, Xu J, Liu J, Taniguchi R, Yastula B, Hsia HC, Qyang Y, Dardik A. The potential and limitations of induced pluripotent stem cells to achieve wound healing.. Stem Cell Res Ther 2019 Mar 12;10(1):87.
    doi: 10.1186/s13287-019-1185-1pmc: PMC6416973pubmed: 30867069google scholar: lookup
  6. Fu X, Li H. Mesenchymal stem cells and skin wound repair and regeneration: possibilities and questions.. Cell Tissue Res 2009 Feb;335(2):317-21.
    doi: 10.1007/s00441-008-0724-3pubmed: 19034523google scholar: lookup
  7. Lopes B, Sousa P, Alvites R, Branquinho M, Sousa A, Mendonça C, Atayde LM, Maurício AC. The Application of Mesenchymal Stem Cells on Wound Repair and Regeneration. Appl. Sci. 2021;11:3000.
    doi: 10.3390/app11073000google scholar: lookup
  8. Iacono E, Merlo B, Pirrone A, Antonelli C, Brunori L, Romagnoli N, Castagnetti C. Effects of mesenchymal stem cells isolated from amniotic fluid and platelet-rich plasma gel on severe decubitus ulcers in a septic neonatal foal.. Res Vet Sci 2012 Dec;93(3):1439-40.
    doi: 10.1016/j.rvsc.2012.04.008pubmed: 22579411google scholar: lookup
  9. Volk SW, Theoret C. Translating stem cell therapies: the role of companion animals in regenerative medicine.. Wound Repair Regen 2013 May-Jun;21(3):382-94.
    doi: 10.1111/wrr.12044pmc: PMC3670702pubmed: 23627495google scholar: lookup
  10. Lanci A, Merlo B, Mariella J, Castagnetti C, Iacono E. Heterologous Wharton's Jelly Derived Mesenchymal Stem Cells Application on a Large Chronic Skin Wound in a 6-Month-Old Filly.. Front Vet Sci 2019;6:9.
    doi: 10.3389/fvets.2019.00009pmc: PMC6363668pubmed: 30761313google scholar: lookup
  11. Ji X, Yuan X, Ma L, Bi B, Zhu H, Lei Z, Liu W, Pu H, Jiang J, Jiang X, Zhang Y, Xiao J. Mesenchymal stem cell-loaded thermosensitive hydroxypropyl chitin hydrogel combined with a three-dimensional-printed poly(ε-caprolactone) /nano-hydroxyapatite scaffold to repair bone defects via osteogenesis, angiogenesis and immunomodulation.. Theranostics 2020;10(2):725-740.
    doi: 10.7150/thno.39167pmc: PMC6929983pubmed: 31903147google scholar: lookup
  12. Tayalia P, Mooney DJ. Controlled growth factor delivery for tissue engineering.. Adv Mater 2009 Sep 4;21(32-33):3269-85.
    doi: 10.1002/adma.200900241pubmed: 20882497google scholar: lookup
  13. Marklein RA, Burdick JA. Controlling stem cell fate with material design.. Adv Mater 2010 Jan 12;22(2):175-89.
    doi: 10.1002/adma.200901055pubmed: 20217683google scholar: lookup
  14. Isomursu A, Lerche M, Taskinen ME, Ivaska J, Peuhu E. Integrin signaling and mechanotransduction in regulation of somatic stem cells.. Exp Cell Res 2019 May 15;378(2):217-225.
    doi: 10.1016/j.yexcr.2019.01.027pubmed: 30817927google scholar: lookup
  15. Campbell ID, Humphries MJ. Integrin structure, activation, and interactions.. Cold Spring Harb Perspect Biol 2011 Mar 1;3(3).
    doi: 10.1101/cshperspect.a004994pmc: PMC3039929pubmed: 21421922google scholar: lookup
  16. Prowse AB, Chong F, Gray PP, Munro TP. Stem cell integrins: implications for ex-vivo culture and cellular therapies.. Stem Cell Res 2011 Jan;6(1):1-12.
    doi: 10.1016/j.scr.2010.09.005pubmed: 21075697google scholar: lookup
  17. Hynes RO. Integrins: bidirectional, allosteric signaling machines.. Cell 2002 Sep 20;110(6):673-87.
    doi: 10.1016/S0092-8674(02)00971-6pubmed: 12297042google scholar: lookup
  18. Baiula M, Galletti P, Martelli G, Soldati R, Belvisi L, Civera M, Dattoli SD, Spampinato SM, Giacomini D. New β-Lactam Derivatives Modulate Cell Adhesion and Signaling Mediated by RGD-Binding and Leukocyte Integrins.. J Med Chem 2016 Nov 10;59(21):9721-9742.
    doi: 10.1021/acs.jmedchem.6b00576pubmed: 27726366google scholar: lookup
  19. Alcaide B, Almendros P, Aragoncillo C. Highly reactive 4-membered ring nitrogen-containing heterocycles: Synthesis and properties.. Curr Opin Drug Discov Devel 2010;13(6):685-97.
    pubmed: 21061231
  20. Greiner A, Wendorff JH. Electrospinning: a fascinating method for the preparation of ultrathin fibers.. Angew Chem Int Ed Engl 2007;46(30):5670-703.
    doi: 10.1002/anie.200604646pubmed: 17585397google scholar: lookup
  21. Wang C, Wang J, Zeng L, Qiao Z, Liu X, Liu H, Zhang J, Ding J. Fabrication of Electrospun Polymer Nanofibers with Diverse Morphologies.. Molecules 2019 Feb 26;24(5).
    doi: 10.3390/molecules24050834pmc: PMC6429487pubmed: 30813599google scholar: lookup
  22. Martelli G, Bloise N, Merlettini A, Bruni G, Visai L, Focarete ML, Giacomini D. Combining Biologically Active β-Lactams Integrin Agonists with Poly(l-lactic acid) Nanofibers: Enhancement of Human Mesenchymal Stem Cell Adhesion.. Biomacromolecules 2020 Mar 9;21(3):1157-1170.
    doi: 10.1021/acs.biomac.9b01550pmc: PMC7997109pubmed: 32011862google scholar: lookup
  23. Iacono E, Pascucci L, Bazzucchi C, Cunto M, Ricci F, Rossi B, Merlo B. Could hypoxia influence basic biological properties and ultrastructural features of adult canine mesenchymal stem /stromal cells?. Vet Res Commun 2018 Dec;42(4):297-308.
    doi: 10.1007/s11259-018-9738-9pubmed: 30238341google scholar: lookup
  24. Merlo B, Pirondi S, Iacono E, Rossi B, Ricci F, Mari G. VIABILITY, IN VITRO DIFFERENTIATION AND MOLECULAR CHARACTERIZATION OF EQUINE ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS CRYOPRESERVED IN SERUM AND SERUM-FREE MEDIUM.. Cryo Letters 2016 Jul Aug;37(4):243-252.
    pubmed: 27925007
  25. Hao D, Ma B, He C, Liu R, Farmer DL, Lam KS, Wang A. Surface modification of polymeric electrospun scaffolds via a potent and high-affinity integrin α4β1 ligand improved the adhesion, spreading and survival of human chorionic villus-derived mesenchymal stem cells: a new insight for fetal tissue engineering.. J Mater Chem B 2020 Feb 26;8(8):1649-1659.
    doi: 10.1039/C9TB02309Gpmc: PMC7353926pubmed: 32011618google scholar: lookup
  26. Foster AP, McCabe PJ, Sanjar S, Cunningham FM. Agonist-induced adherence of equine eosinophils to fibronectin.. Vet Immunol Immunopathol 1997 May;56(3-4):205-20.
    doi: 10.1016/S0165-2427(96)05740-6pubmed: 9223226google scholar: lookup
  27. Al-Ramadan SY, Brinsko P, Rigby SL, Jaegera LA, Burghardt RC. Analysis of MUC-1 and Integrin Subunit Expression in Equine Uterine Epithelium and Trophectoderm. Theriogenology 2002;58:829–832.
    doi: 10.1016/S0093-691X(02)00819-1google scholar: lookup
  28. Grant DM, Macedo A, Toms D, Klein C. Fibrinogen in equine pregnancy as a mediator of cell adhesion, an epigenetic and functional investigation.. Biol Reprod 2020 Feb 12;102(1):170-184.
    doi: 10.1093/biolre/ioz157pubmed: 31403677google scholar: lookup
  29. Treonze KM, Alves K, Fischer P, Hagmann WK, Hora D, Kulick A, Vakerich K, Smith ND, Lingham RB, Maniar S, Reger TS, Zunic J, Munoz B, Prasit P, Nicholson D, Si Q, Judd K, Nicolich S, Kellerhouse P, Thompson D, Mumford RA. Characterization of alpha(4)beta(1) (CD49d/CD29) on equine leukocytes: potential utility of a potent alpha(4)beta(1) (CD49d/CD29) receptor antagonist in the treatment of equine heaves (recurrent airway obstruction).. Vet Immunol Immunopathol 2009 Jul 15;130(1-2):79-87.
    doi: 10.1016/j.vetimm.2009.01.011pubmed: 19250687google scholar: lookup
  30. Claessen C, Favoreel H, Ma G, Osterrieder N, De Schauwer C, Piepers S, Van de Walle GR. Equid herpesvirus 1 (EHV1) infection of equine mesenchymal stem cells induces a pUL56-dependent downregulation of select cell surface markers.. Vet Microbiol 2015 Mar 23;176(1-2):32-9.
    doi: 10.1016/j.vetmic.2014.12.013pubmed: 25582614google scholar: lookup
  31. Laval K, Favoreel HW, Poelaert KC, Van Cleemput J, Nauwynck HJ. Equine Herpesvirus Type 1 Enhances Viral Replication in CD172a+ Monocytic Cells upon Adhesion to Endothelial Cells.. J Virol 2015 Nov;89(21):10912-23.
    doi: 10.1128/JVI.01589-15pmc: PMC4621108pubmed: 26292328google scholar: lookup
  32. Kolyvushko O, Kelch MA, Osterrieder N, Azab W. Equine Alphaherpesviruses Require Activation of the Small GTPases Rac1 and Cdc42 for Intracellular Transport.. Microorganisms 2020 Jul 7;8(7).
    doi: 10.3390/microorganisms8071013pmc: PMC7409331pubmed: 32645930google scholar: lookup
  33. Baiula M, Spampinato S, Gentilucci L, Tolomelli A. Novel Ligands Targeting α(4)β(1) Integrin: Therapeutic Applications and Perspectives.. Front Chem 2019;7:489.
    doi: 10.3389/fchem.2019.00489pmc: PMC6629825pubmed: 31338363google scholar: lookup
  34. Grassinger J, Haylock DN, Storan MJ, Haines GO, Williams B, Whitty GA, Vinson AR, Be CL, Li S, Sørensen ES, Tam PP, Denhardt DT, Sheppard D, Choong PF, Nilsson SK. Thrombin-cleaved osteopontin regulates hemopoietic stem and progenitor cell functions through interactions with alpha9beta1 and alpha4beta1 integrins.. Blood 2009 Jul 2;114(1):49-59.
    doi: 10.1182/blood-2009-01-197988pubmed: 19417209google scholar: lookup
  35. Vanderslice P, Biediger RJ, Woodside DG, Brown WS, Khounlo S, Warier ND, Gundlach CW 4th, Caivano AR, Bornmann WG, Maxwell DS, McIntyre BW, Willerson JT, Dixon RA. Small molecule agonist of very late antigen-4 (VLA-4) integrin induces progenitor cell adhesion.. J Biol Chem 2013 Jul 5;288(27):19414-28.
    doi: 10.1074/jbc.M113.479634pmc: PMC3707645pubmed: 23703610google scholar: lookup
  36. Colleoni S, Bottani E, Tessaro I, Mari G, Merlo B, Romagnoli N, Spadari A, Galli C, Lazzari G. Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor.. Vet Res Commun 2009 Dec;33(8):811-21.
    doi: 10.1007/s11259-009-9229-0pubmed: 19472068google scholar: lookup
  37. Bagge J, MacLeod JN, Berg LC. Cellular Proliferation of Equine Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells Decline With Increasing Donor Age.. Front Vet Sci 2020;7:602403.
    doi: 10.3389/fvets.2020.602403pmc: PMC7758322pubmed: 33363241google scholar: lookup
  38. LaFlamme SE, Nieves B, Colello D, Reverte CG. Integrins as regulators of the mitotic machinery.. Curr Opin Cell Biol 2008 Oct;20(5):576-82.
    doi: 10.1016/j.ceb.2008.06.006pmc: PMC3417292pubmed: 18621126google scholar: lookup
  39. Lechler T, Fuchs E. Asymmetric cell divisions promote stratification and differentiation of mammalian skin.. Nature 2005 Sep 8;437(7056):275-80.
    doi: 10.1038/nature03922pmc: PMC1399371pubmed: 16094321google scholar: lookup
  40. Toyoshima F, Nishida E. Integrin-mediated adhesion orients the spindle parallel to the substratum in an EB1- and myosin X-dependent manner.. EMBO J 2007 Mar 21;26(6):1487-98.
    doi: 10.1038/sj.emboj.7601599pmc: PMC1829369pubmed: 17318179google scholar: lookup
  41. Mahdavi FS, Salehi A, Seyedjafari E, Mohammadi-Sangcheshmeh A, Ardeshirylajimi A. Bioactive glass ceramic nanoparticles-coated poly(l-lactic acid) scaffold improved osteogenic differentiation of adipose stem cells in equine.. Tissue Cell 2017 Oct;49(5):565-572.
    doi: 10.1016/j.tice.2017.07.003pubmed: 28851519google scholar: lookup
  42. Duan W, Chen C, Haque M, Hayes D, Lopez MJ. Polymer-mineral scaffold augments in vivo equine multipotent stromal cell osteogenesis.. Stem Cell Res Ther 2018 Mar 9;9(1):60.
    doi: 10.1186/s13287-018-0790-8pmc: PMC5845133pubmed: 29523214google scholar: lookup
  43. Bageshlooyafshar B, Vakilian S, Kehtari M, Eslami-Arshaghi T, Rafeie F, Ramezanifard R, Rahchamani R, Mohammadi-Sangcheshmeh A, Mostafaloo Y, Seyedjafari E. Zinc silicate mineral-coated scaffold improved in vitro osteogenic differentiation of equine adipose-derived mesenchymal stem cells.. Res Vet Sci 2019 Jun;124:444-451.
    doi: 10.1016/j.rvsc.2017.09.015pubmed: 29031416google scholar: lookup
  44. Zhao L, Detamore MS. Chondrogenic differentiation of stem cells in human umbilical cord stroma with PGA and PLLA scaffolds.. J Biomed Sci Eng 2010 Nov;3(11):1041-1049.
    doi: 10.4236/jbise.2010.311135pmc: PMC4474381pubmed: 26097626google scholar: lookup

Citations

This article has been cited 3 times.
  1. Ferrero I, Proto CF, Banche Niclot AGS, Marini E, Pascucci L, Piccinini F, Mareschi K. State of the Art and New Trends from the 2022 Gism Annual Meeting. Int J Mol Sci 2023 May 17;24(10).
    doi: 10.3390/ijms24108902pubmed: 37240248google scholar: lookup
  2. Baldassarro VA, Giraldi V, Giuliani A, Moretti M, Pagnotta G, Flagelli A, Clavenzani P, Lorenzini L, Giardino L, Focarete ML, Giacomini D, Calzà L. Poly(l-lactic acid) Scaffold Releasing an α(4)β(1) Integrin Agonist Promotes Nonfibrotic Skin Wound Healing in Diabetic Mice. ACS Appl Bio Mater 2023 Jan 16;6(1):296-308.
    doi: 10.1021/acsabm.2c00890pubmed: 36542733google scholar: lookup
  3. He T, Giacomini D, Tolomelli A, Baiula M, Gentilucci L. Conjecturing about Small-Molecule Agonists and Antagonists of α4β1 Integrin: From Mechanistic Insight to Potential Therapeutic Applications. Biomedicines 2024 Jan 30;12(2).
    doi: 10.3390/biomedicines12020316pubmed: 38397918google scholar: lookup
Subscribe to our newsletter
Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.