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Animals : an open access journal from MDPI2022; 12(6); 734; doi: 10.3390/ani12060734

Peptide Mediated Adhesion to Beta-Lactam Ring of Equine Mesenchymal Stem Cells: A Pilot Study.

Abstract: Regenerative medicine applied to skin lesions is a field in constant improvement. The use of biomaterials with integrin agonists could promote cell adhesion increasing tissue repair processes. The aim of this pilot study was to analyze the effect of an α4β1 integrin agonist on cell adhesion of equine adipose tissue (AT) and Wharton's jelly (WJ) derived MSCs and to investigate their adhesion ability to GM18 incorporated poly L-lactic acid (PLLA) scaffolds. Adhesion assays were performed after culturing AT- and WJ-MSCs with GM18 coating or soluble GM18. Cell adhesion on GM18 containing PLLA scaffolds after 20 min co-incubation was assessed by HCS. Soluble GM18 affects the adhesion of equine AT- and WJ-MSCs, even if its effect is variable between donors. Adhesion to PLLA scaffolds containing GM18 is not significantly influenced by GM18 for AT-MSCs after 20 min or 24 h of culture and for WJ-MSCs after 20 min, but increased cell adhesion by 15% GM18 after 24 h. In conclusion, the α4β1 integrin agonist GM18 affects equine AT- and WJ-MSCs adhesion ability with a donor-related variability. These preliminary results represent a first step in the study of equine MSCs adhesion to PLLA scaffolds containing GM18, suggesting that WJ-MSCs might be more suitable than AT-MSCs. However, the results need to be confirmed by increasing the number of samples before drawing definite conclusions.
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Publication Date: 2022-03-15 PubMed ID: 35327131PubMed Central: PMC8944785DOI: 10.3390/ani12060734Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the use of an α4β1 integrin agonist on the cell adhesion of equine adipose tissue and Wharton’s jelly-derived Mesenchymal Stem Cells (MSCs) to improve tissue repair processes in the field of regenerative medicine. The findings suggest that the α4β1 integrin agonist GM18 can variably affect cell adhesion ability, indicating that further research is needed.

Objective of the Research

  • This pilot study was aimed at understanding how an α4β1 integrin agonist (GM18) could impact cell adhesion of equine adipose tissue (AT) and Wharton’s jelly (WJ) derived MSCs.
  • The researchers also sought to investigate the adhesion ability of these stem cells to GM18 incorporated poly L-lactic acid (PLLA) scaffolds.

Methodology of the Research

  • The team performed adhesion assays after culturing AT and WJ-MSCs with a coating of GM18 or a solution of GM18.
  • The cell adhesion on GM18 containing PLLA scaffolds was assessed after a co-incubation period of 20 minutes through high-content screening (HCS).

Findings of the Research

  • The researchers observed that soluble GM18 affects the adhesion of both equine AT and WJ-MSCs, with the impact varying between donors.
  • Adhesion to PLLA scaffolds containing GM18 does not significantly change for AT-MSCs after either 20 minutes or 24 hours of culture, or for WJ-MSCs after 20 minutes.
  • However, there was an increase in cell adhesion by 15% for GM18 with WJ-MSCs after 24 hours.

Conclusion of the Research

  • Overall, GM18, an α4β1 integrin agonist, appears to impact the adhesion ability of equine AT and WJ-MSCs with variability that depends on the donor.
  • The preliminary results point towards WJ-MSCs potentially being more suitable than AT-MSCs for adhesion to PLLA scaffolds containing GM18.
  • Despite these initial findings, the researchers acknowledge that the results need to be confirmed through additional testing with a greater number of samples before any conclusive arguments can be made.

Cite This Article

APA
(2022). Peptide Mediated Adhesion to Beta-Lactam Ring of Equine Mesenchymal Stem Cells: A Pilot Study. Animals (Basel), 12(6), 734. https://doi.org/10.3390/ani12060734

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 6
PII: 734

Researcher Affiliations

Conflict of Interest Statement

The authors declare no conflict of interest.

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