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Cytotechnology2014; 68(4); 795-807; doi: 10.1007/s10616-014-9831-z

Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood.

Abstract: Mesenchymal stem cells (MSCs) offer promise as therapeutic aid in the repair of tendon and ligament injuries in race horses. Fetal adnexa is considered as an ideal source of MSCs due to many advantages, including non-invasive nature of isolation procedures and availability of large tissue mass for harvesting the cells. However, MSCs isolated from equine fetal adnexa have not been fully characterized due to lack of species-specific markers. Therefore, this study was carried out to isolate MSCs from equine umbilical cord blood (UCB) and characterize them using cross-reactive markers. The plastic-adherent cells could be isolated from 13 out of 20 (65 %) UCB samples. The UCB derived cells proliferated till passage 20 with average cell doubling time of 46.40 ± 2.86 h. These cells expressed mesenchymal surface markers but did not express haematopoietic/leucocytic markers by RT-PCR and immunocytochemistry. The phenotypic expression of CD29, CD44, CD73 and CD90 was shown by 96.36 ± 1.28, 93.40 ± 0.70, 73.23 ± 1.29 and 46.75 ± 3.95 % cells, respectively in flow cytometry, whereas, reactivity against the haematopoietic antigens CD34 and CD45 was observed only in 2.4 ± 0.20 and 0.1 ± 0.0 % of cells, respectively. Osteogenic and chondrogenic differentiation could be achieved using established methods, whereas the optimum adipogenic differentiation was achieved after supplementing media with 15 % rabbit serum and 20 ng/ml of recombinant human insulin. In this study, we optimized methodology for isolation, cultural characterization, differentiation and immunophenotyping of MSCs from equine UCB. Protocols and markers used in this study can be employed for unequivocal characterization of equine MSCs.
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Publication Date: 2014-12-09 PubMed ID: 25487085PubMed Central: PMC4960129DOI: 10.1007/s10616-014-9831-zGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article discusses the characteristics and functions of mesenchymal stem cells (MSCs) obtained from equine umbilical cord blood, and their potential use in the treatment of ligament and tendon injuries in race horses.

Overview of the Research

  • The research focuses on stem cells derived from the umbilical cord blood of horses. The main goal of the study is to isolate these cells and understand their characteristics and ability to multiply under given conditions.
  • The study evaluated the growth of the isolated cells up to the 20th passage and measured the time taken for each cell to divide.
  • The markers expressed on the surfaces of these cells were also evaluated to confirm if they were MSCs.

Key Findings

  • The authors managed to isolate plastic-adherent cells from 65% of the umbilical cord blood samples. These cells could grow and multiply until the 20th passage with an average doubling time of approximately 46 hours.
  • The cells examined expressed mesenchymal surface markers (proteins expressed on the surface of the cells), specifically CD29, CD44, CD73, and CD90.
  • These cells were found not to express haematopoietic/leucocytic markers, making them distinct from cells that contribute to the formation of blood cell components.
  • When subjected to specific conditions, these cells were shown to be capable of differentiating into bone cells (osteogenic differentiation), cartilage cells (chondrogenic differentiation), and fat cells (adipogenic differentiation), highlighting their versatility.

Conclusion and Implications

  • The study concludes that methods have been optimized for the isolation, cultural characterization, differentiation, and immunophenotyping (analysis of the protein expressed on the surface of the cells) of the MSCs derived from equine umbilical cord blood.
  • These protocols and markers can provide a definitive method of characterizing equine MSCs, displaying their potential usefulness in therapeutic applications such as tissue repair.
  • Given the non-invasive nature of MSCs isolation and its potential therapeutic implications, further research in this area could potentially pave the way for innovative treatments in equine medicine.

Cite This Article

APA
Mohanty N, Gulati BR, Kumar R, Gera S, Kumar S, Kumar P, Yadav PS. (2014). Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood. Cytotechnology, 68(4), 795-807. https://doi.org/10.1007/s10616-014-9831-z

Publication

Cytotechnology
ISSN: 0920-9069
NlmUniqueID: 8807027
Country: United States
Language: English
Volume: 68
Issue: 4
Pages: 795-807

Researcher Affiliations

Mohanty, N
  • Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India.
Gulati, B R
  • National Research Centre on Equines, Sirsa Road, Hisar, 125001, Haryana, India. brgulati@gmail.com.
Kumar, R
  • National Research Centre on Equines, Sirsa Road, Hisar, 125001, Haryana, India.
Gera, S
  • Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India.
Kumar, S
  • Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India.
Kumar, P
  • Department of Veterinary Anatomy, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India.
Yadav, P S
  • Central Institute for Research on Buffaloes, Sirsa Road, Hisar, 125001, Haryana, India.

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Citations

This article has been cited 5 times.
  1. Petrova V, Yonkova P, Simeonova G, Vachkova E. Horse serum potentiates cellular viability and improves indomethacin-induced adipogenesis in equine subcutaneous adipose-derived stem cells (ASCs).. Int J Vet Sci Med 2023;11(1):94-105.
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