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Home / Equine Research Bank / Trop Anim Health Prod / Phylogenetic analysis of West Nile virus: first report of li...
Tropical animal health and production2021; 53(5); 453; doi: 10.1007/s11250-021-02892-z

Phylogenetic analysis of West Nile virus: first report of lineage 1 in donkey in Turkey.

Abstract: West Nile virus (WNV), a member of the Flaviviridae, is a major arbovirus that causes West Nile fever. Previous data showed the prevalence of the WNV serologically and molecular in Turkey, and the presence of lineage 1 in horses and humans has been reported. This is the first notification of partial phylogeny of WNV detected in donkeys in the northeast of Turkey (on the Iranian border). Blood serum samples collected from 25 donkeys without clinical symptoms were tested by RT-PCR. Sequence analysis of the sample detected as positive was performed. Multiple sequence alignments of reference sequences taken from GenBank were performed using the ClustalW method using the MEGA6 program. Partial nucleotide sequences of the capsid gene coding region revealed that the strains are closely related to viruses of lineage 1, clade 1a. According to the phylogenetic tree, the TUR/Igdir/donkey strain was included in the same cluster as the strain (KJ958922) previously obtained from horses in Turkey and the strain (GQ851658) from the Central African Republic. This study is the first report to show the circulation of WNV lineage 1 in donkeys in Turkey.
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.
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Publication Date: 2021-09-18 PubMed ID: 34536145PubMed Central: 6522068DOI: 10.1007/s11250-021-02892-zGoogle Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigated the presence of a certain strain of West Nile virus (WNV), lineage 1, in donkeys in Turkey for the first time.

Objective and Importance of the Study

  • The study dealt with the investigation of the presence and circulation of West Nile virus’ (WNV) lineage 1 in donkeys in Turkey. West Nile virus (WNV), a major arbovirus, causes West Nile fever. The understanding of it’s distribution and hosts is important for public health and veterinary medicine.
  • Though the presence of lineage 1 in horses and humans had been detected in Turkey, this is the first report of the detection of this viral lineage in donkeys in the country.

Methodology

  • Blood serum samples were collected from 25 donkeys presenting no clinical symptoms.
  • These samples were tested using the RT-PCR technique, a technique involving reverse transcription of viral RNA into DNA and its amplification to aid in the detection of the virus.
  • The sequence analysis of the identified positive sample was carried out.
  • For comparison, reference sequences were used that were obtained from GenBank, an online genetic sequence database.
  • The sequence alignments were carried out using the ClustalW method through the MEGA6 program.

Findings

  • The sequence analysis and alignment revealed that the virus strains in the donkey samples were closely related to lineage 1, clade 1a of the WNV.
  • In the phylogenetic tree (a diagram showing the relationship between different organisms based on their genetic similarity), the Donkey strain (TUR/Igdir/donkey) clustered with a specific strain detected earlier in horses in Turkey (KJ958922) and a strain found in the Central African Republic (GQ851658).

Conclusions

  • The study concluded with the discovery that WNV lineage 1 circulates in donkeys in Turkey, a finding recorded for the first time.
  • This discovery is significant for understanding the spread and hosts of WNV lineage 1, contributing to better preventive measures and treatments for West Nile fever.

Cite This Article

APA
Yıldırım Y, Yılmaz V, Yazıcı K, Öziç C, Ozkul A, Çağırgan AA. (2021). Phylogenetic analysis of West Nile virus: first report of lineage 1 in donkey in Turkey. Trop Anim Health Prod, 53(5), 453. https://doi.org/10.1007/s11250-021-02892-z

Publication

Tropical animal health and production
ISSN: 1573-7438
NlmUniqueID: 1277355
Country: United States
Language: English
Volume: 53
Issue: 5
Pages: 453

Researcher Affiliations

Yıldırım, Yakup
  • Department of Virology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, 15100, Turkey. yayildirim@hotmail.com.
Yılmaz, Volkan
  • Department of Virology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey.
Yazıcı, Kemal
  • Vocational School of Technical Sciences, Ardahan University, Ardahan, Turkey.
Öziç, Cem
  • Department of Bioengineering, Faculty of Engineering-Architecture, Kafkas University, Kars, Turkey.
Ozkul, Aykut
  • Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
Çağırgan, Abdurrahman Anıl
  • Department of Virology, Izmir/Bornova Veterinary Control Institute, Izmir, Turkey.

MeSH Terms

  • Animals
  • Equidae
  • Horses
  • Iran / epidemiology
  • Phylogeny
  • Turkey / epidemiology
  • West Nile virus / genetics

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Citations

This article has been cited 1 times.
  1. Mencattelli G, Iapaolo F, Monaco F, Fusco G, de Martinis C, Portanti O, Di Gennaro A, Curini V, Polci A, Berjaoui S, Di Felice E, Rosà R, Rizzoli A, Savini G. West Nile Virus Lineage 1 in Italy: Newly Introduced or a Re-Occurrence of a Previously Circulating Strain?. Viruses 2021 Dec 30;14(1).
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