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Home / Equine Research Bank / Animals (Basel) / Plasma and Synovial Fluid Cell-Free DNA Concentrations Follo...
Animals : an open access journal from MDPI2023; 13(6); 1053; doi: 10.3390/ani13061053

Plasma and Synovial Fluid Cell-Free DNA Concentrations Following Induction of Osteoarthritis in Horses.

Abstract: Biomarkers for osteoarthritis (OA) in horses have been extensively investigated, but translation into clinical use has been limited due to cost, limited sensitivity, and practicality. Identifying novel biomarkers that overcome these limitations could facilitate early diagnosis and therapy. This study aimed to compare the concentrations of synovial fluid (SF) and plasma cell-free DNA (cfDNA) over time in control horses with those with induced carpal OA. Following an established model, unilateral carpal OA was induced in 9 of 17 healthy Thoroughbred fillies, while the remainder were sham-operated controls. Synovial fluid and plasma samples were obtained before induction of OA (Day 0) and weekly thereafter until Day 63, and cfDNA concentrations were determined using fluorometry. The SF cfDNA concentrations were significantly higher for OA joints than for sham-operated joints on Days 28 (median 1430 μg/L and 631 μg/L, respectively, = 0.017) and 63 (median 1537 μg/L and 606 μg/L, respectively, = 0.021). There were no significant differences in plasma cfDNA between the OA and the sham groups after induction of carpal OA. Plasma cfDNA measurement is not sufficiently sensitive for diagnostic purposes in this induced model of OA. Synovial fluid cfDNA measurement may be used as a biomarker to monitor early disease progression in horses with OA.
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Publication Date: 2023-03-14 PubMed ID: 36978592PubMed Central: PMC10044647DOI: 10.3390/ani13061053Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study examines the potential of synovial fluid and plasma cell-free DNA (cfDNA) as effective biomarkers for the early detection and monitoring of osteoarthritis (OA) in horses. Their research found that cfDNA levels were significantly high in the synovial fluid of horses with induced OA but saw no significant differences in plasma cfDNA levels.

Study Design and Procedure

  • The study involved a total of 17 healthy Thoroughbred fillies. In these, unilateral carpal OA was induced in nine horses, while the rest were used as control subjects who underwent sham operations.
  • Prior to the induction of OA (Day 0) and weekly afterward until Day 63, samples of synovial fluid and plasma were collected from all the horses. This was done in order to compare the concentration of cfDNA over time.
  • The concentration of cfDNA was determined using fluorometry, a technique that measures the concentration of fluorescent particles.

Findings of the Study

  • The research found a notable increase in the concentration of cfDNA in the synovial fluid of the horses in which OA was induced. The median amounts were significantly higher on Days 28 and 63 compared to the control group horses.
  • Contrary to synovial fluid, the plasma samples showed no significant differences in cfDNA concentrations between the horses with induced OA and the control group.

Conclusions and Implications

  • The results of the study suggest that synovial fluid cfDNA can be potentially used as a biomarker to monitor the early stages and progression of OA in horses.
  • However, the study found plasma cfDNA levels to be insufficiently sensitive for diagnostic purposes for OA in the induced model, thus ruling out its use as a biomarker.
  • The study’s findings, if further validated, could pave the way for the development of cost-effective and convenient diagnostic methods for OA in equine veterinary medicine.

Cite This Article

APA
Panizzi L, Dittmer KE, Vignes M, Doucet JS, Gedye K, Waterland MR, Rogers CW, Sano H, McIlwraith CW, Riley CB. (2023). Plasma and Synovial Fluid Cell-Free DNA Concentrations Following Induction of Osteoarthritis in Horses. Animals (Basel), 13(6), 1053. https://doi.org/10.3390/ani13061053

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 6
PII: 1053

Researcher Affiliations

Panizzi, Luca
  • School of Veterinary Science, College of Science, Massey University, Palmerston North 4442, New Zealand.
Dittmer, Keren E
  • School of Veterinary Science, College of Science, Massey University, Palmerston North 4442, New Zealand.
Vignes, Matthieu
  • School of Mathematical and Computational Sciences, College of Science, Massey University, Palmerston North 4442, New Zealand.
Doucet, Jennie S
  • Department of Biology, Faculty of Science, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Gedye, Kristene
  • School of Veterinary Science, College of Science, Massey University, Palmerston North 4442, New Zealand.
Waterland, Mark R
  • School of Natural Sciences, College of Science, Massey University, Palmerston North 4442, New Zealand.
Rogers, Chris W
  • School of Veterinary Science, College of Science, Massey University, Palmerston North 4442, New Zealand.
  • School of Agriculture and Environment, College of Science, Massey University, Palmerston North 4442, New Zealand.
Sano, Hiroki
  • School of Veterinary Science, College of Science, Massey University, Palmerston North 4442, New Zealand.
McIlwraith, C Wayne
  • Orthopaedic Research Center, C. Wayne McIlwraith Translational Medicine Institute, School of Veterinary Medicine, Colorado State University, Fort Collins, CO 80523-1601, USA.
Riley, Christopher B
  • School of Veterinary Science, College of Science, Massey University, Palmerston North 4442, New Zealand.
  • Department of Clinical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

Grant Funding

  • N/A / New Zealand Equine Trust, Massey University
  • N/A / Lewis Fitch Veterinary Research Fund, Massey University

Conflict of Interest Statement

One of the authors (C.W.M) is the Chair of the New Zealand Equine Trust. C.W.M. had no role in the design of the study; in the collection, analysis, or interpretation of data or in the decision to publish the results. C.W.M. provided surgical expertise to ensure consistency of the OA model previously established and revised the final version of the manuscript.

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